Optimation of Hematopoietic Stem Cells’ Culture Medium: A Review

prasetyadi prasetyadi

= http://dx.doi.org/10.20473/jscrte.v3i2.20129
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Abstract


Hematopoietic stem cells (HSCs) usage has numerous potential benefits for basic and clinical research.  Growing interest in this field is caused by possible HSCs application   to cure   various   hematologic   diseases, malignancies, immunodeficiency diseases, and inborn errors of metabolism.  Although the demand of HSCs is increasing, ex vivo culture of HSCs has various medium compositions. This article focuses on HSCs’ culture medium optimation in different medium supplements, which might overcome non-optimal HSCs culture results. Literature searching was conducted in PubMed and google scholar database. Paper selection processes were done by author separately and studies which met the eligible criteria were included in this review. A total of 53 relevant articles were identified and 11 articles that met the eligible criteria were included in this review. There are two main types of supplement, cytokines and non-cytokines.  In cytokines addition, 7 studies generally show that the supplement support HSCs expansion.  The addition of non-cytokines supplements has more diverse   result in studies.   Positive   results   with various   effectivity   in CD34+ expansion were shown in 3 studies, while one study shows a negative result. Medium supplementation must be explored more to find best substances which can optimize   HSCs   culture.   Further   research   using   non-cytokines   substance   shall   be conducted regarding its various effect and might give us a big opportunity to find an optimal condition for HSCs culture.


Keywords


Hematopoietic stem cells, culture medium supplementation, CD34+

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References


Angchaisuksiri P, Carlson P, Dessypris E. Effects of recombinant human thrombopoietin on megakaryocyte colony formation and megakaryocyte ploidy by human CD34+cells in a serum-free system. British Journal of Haematology. 1996;93(1):13-7.

Desplat V, Ivanovic Z, Dupuis F, Faucher J, Denizot Y, Praloran V. Effects of Lipoxygenase Metabolites of Arachidonic Acid on the Growth of Human Blood CD34+ Progenitors. Blood Cells, Molecules, and Diseases. 2000;26(5):427-436.

Du Z, Cai H, Ye Z, Tan W. Optimization of SCF feeding regimen for ex vivo expansion of cord blood hematopoietic stem cells. Journal of Biotechnology. 2013;164(2):211-9.

Duchez P, Chevaleyre J, Vlaski M, Dazey B, Bijou F, Lafarge X et al. Thrombopoietin to replace megakaryocyte-derived growth factor: impact on stem and progenitor cells during ex vivo expansion of CD34+ cells mobilized in peripheral blood. Transfusion. 2010;51(2):313-8.

Gstraunthaler, Gerhard. Alternatives to the use of fetal bovine serum: Serum- free cell culture. ALTEX. 2003; 20. 275-81.

Ireland J, Podsakoff G, Kohn D, Engel B. 319. Comparison of Serum-Free Media for Optimal Ex Vivo Transduction of Human CD34+ Cells. Molecular Therapy. 2006;13:S122.

Kwok Y, Tang M, Law H, Ngai C, Lau Y, Lau E. Maternal plasma or human serum albumin in wash buffer enhances enrichment and ex vivo expansion of human umbilical cord blood CD34+cells. British Journal of Haematology. 2007;137(5):468-474.

Lansdorp P. Long-term erythropoiesis from constant numbers of CD34+ cells in serum- free cultures initiated with highly purified progenitor cells from human bone marrow. Journal of Experimental Medicine. 1992;175(6):1501-9.

Liu Y, Zhang B, Zhang J, Wang S, Yao H, He L et al. CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro. Science China Life Sciences. 2014;57(2):188-94.

Park B, Yoo K, Kim C. Hematopoietic stem cell expansion and generation: the ways to make a breakthrough. Blood Research. 2015;50(4):194.

Piacibello W, Gammaitoni L, Bruno S, Gunetti M, Fagioli F, Cavalloni G et al. Negative Influence of IL3 on the Expansion of Human Cord Blood In Vivo Long-Term Repopulating Stem Cells. Journal of Hematotherapy & Stem Cell Research. 2000;9(6):945- 56.

Qu Q, Liu L, Chen G, Xu Y, Wu X, Wu D. Endothelial progenitor cells promote efficient ex vivo expansion of cord blood–derived hematopoietic stem/progenitor cells. Cytotherapy 2016;18(3):452-64.

Rofani C, Luchetti L, Testa G, Lasorella R, Isacchi G, Bottazzo G et al. IL-16 Can Synergize With Early Acting Cytokines to Expand Ex Vivo CD34+Isolated from Cord Blood. Stem Cells and Development. 2009;18(4):671-82.

Technical bulletin culture of hematopoietic stem and progenitor cells. Stemcell.com. 2018

Yao C, Chu I, Hsieh T, Hwang S. A systematic strategy to optimize ex vivo expansion medium for human hematopoietic stem cells derived from umbilical cord blood mononuclear cells. Experimental Hematology. 2004;32(8):720-727.

Wu M, Smith S, Danet G, Lin A, Williams S, Liebowitz D et al. Optimization of culture conditions to enhance transfection of human CD34+ cells by electroporation. Bone Marrow Transplantation. 2001;27(11):1201- 1209.

Young JC, Bruno E, Luens KM, Wu S, Backer M, Murray LJ. Thrombopoietin Stimulates Megakaryocytopoiesis, Myelopoiesis, and Expansion of CD34+ Progenitor Cells From Single CD34+Thy-l+Lin Primitive Progenitor Cells. Blood. 1996;88(5):1619-31.


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