Injectable Bone Substitute Paste Based on Hydroxyapatite, Gelatin and Streptomycin for Spinal Tuberculosis

hendita maulida

= http://dx.doi.org/10.20473/jscrte.v3i2.20133
Abstract views = 415 times | downloads = 309 times

Abstract


World Health Organization (WHO) in 2005 reported that cases of tuberculosis (TB) in the world occur more than 8 million annually and 5-10% were attacked in spine. The most effective treatment of spinal TB is evacuation of infected bone segments and fill with bone graft. It has been synthesized and characterized of injectable bone substitute (IBS) paste based on hydroxyapatite, gelatin and streptomycin. IBS paste synthesized by mixing hydroxyapatite and gelatin 20% w/v with 75:25, 70:30, 65:35 and 60:40 ratio and streptomycin 10 wt%. The mixture was then added with hydroxypropyl methylcellulose (HPMC) 4% w/v as suspending agent. In vitro characterization performed include acidity (pH), injectability test, setting time, cytotoxicity (MTT assay) and microbacterium test. Acidity test results indicate a fourth variation of the samples had pH values approaching normal body pH (7,3 to 7,6) and is able to maintain stability when measured in 7 days. Injectability test results indicate IBS paste is injectable with the highest percentage of the injectability value at 97,74% ± 0,19%. IBS paste has been setting within 30 minutes to 1 hour when injected on hydroxyapatite scaffold that resembles the bone cavity and is able to cover the pore scaffold seen from the Scanning Electron Microscope (SEM). Scaffold pore size is smaller from range of 780,8 to 835,4 μm into 225,2 μm. MTT assay results showed that IBS paste is not toxic and experiencing proliferation (viability >100%) that are expected to trigger osteoblast cell growth when applied. Microbacterium test results showed that IBS paste is an antibacterial seen from inhibition zone diameter of Staphylococcus aureus and has a high strength-sensitive antibacterial. Thus, hydroxyapatite, gelatin and streptomycin composites had qualified as injectable bone substitute which applied in cases of spinal tuberculosis.


Keywords


Spinal Tuberculosis, Injectable Bone Substitute, Hydroxyapatite, Gelatin, Streptomycin, Hydroxypropyl Methylcellulose

Full Text:

PDF

References


Bramastya, Yusuf. 2013. Effect of Gamma Radiation on Mechanical Properties of UHMWPE-HAp Composite Results from Integrating Mechanical and Felts Heat To Tibial Tray. Bogor, Indonesia: Bogor Agricultural Institute.

Budiatin Aniek Setiya, Muhamad Zaninuddin, Ferdiansyah, Fathia Rachmadani. 2012. Matrix Composites Bovine Hydroxyapatite–Gelatin Effectivity for Gentamicin Delivery System and Regeneration Bone Defect. Surabaya, Indonesia: Airlangga University.

Dorozkhin, Sergey V. 2011. Medical Application of Calcium Orthophosphate Bioceramics. Moscow, Russia. DOI:10.5618/bio.2011.v1.n1.1.

Jawetz, Melnick, Adelberg. 2007. Medical Microbiology, Twenty-Fourth Edition. USA: The Mc-Graw Hills Companies, Pp. 209-216.

Martindale. 2009. The Complete Drug Reference. Thirty-Sixth Edition. United Kingdom: Pharmaceutical Press London. ISBN 978 0 85369 840 1. ISSN 0263-5364.

Mehta J.S. and S.Y. Bhojraj. 2001. Tuberculosis of The Thoracic Spine: A Classification Based On The Selection of Surgical Strategies. India: Hinduja Hospital Mahim Mumbai. The Journal of Bone and Joint Surgery, Vol. 83-B, No. 6.

Moesbar, Nazar. 2006. Infection of Tuberculosis in Spinal. Medan, Indonesia: Departemen of Surgery, Faculty of Medicine, University of Sumatera Utara.

Mutia Theresia, Rifaida Eriningsih, Ratu Safitri. 2011. Alginate Membrane as Primary Wound Dressing and Drug Delivery Media of Topical Medications for Infected Wounds. Bandung, Indonesia: Padjadjaran University. Journal of Industry Research. Vol. V, No.2, 2011, Pp. 161-174.

Paramarta I Gede Epi, Putu Siadi Purniti, Ida Bagus Subanada, Putu Astawa. 2008. Tuberculosis Spondylitis. Denpasar, Indonesia: Faculty of Medicine, Udayana University.

Putra, Alfian. 2014. Synthesis and Characterization of Composite Suspension of Hydroxyapatite- Gelatin with Addition Alendornate for Injectable Bone Substitute. Surabaya, Indonesia: Airlangga University.

Rowe Raymond C, Paul J. Sheskey, Marian E. Quinn. 2009. Handbook Of Pharmaceutical Excipients, Sixth Edition. USA: Pharmaceutical Press, Pp. 326-329.

Shen Zhonghua, Tao Yu, Jiandong Ye. 2014. Microstructure and Properties of Alendronate-Loaded Calcium Phosphate Cement. Journal Elsevier. Materials Science and Engineering C 42 (2014) 303–311.

Spielmann Horst, Andrea Seiler, Susanne Bremer, Lars Hareng, Thomas Hartung, Hans Ahr, Elaine Faustman, Ulla Haas, Graeme J. Moffat, Heinz Nau, Philippe Vanparys, Aldert Piersma, Juan Riego Sintes and Jane Stuart. 2006. The Practical Application Of Three Validated In Vitro Embryotoxicity Tests. ATLA 34, 527-538.

Suryani Nelly, Farida Sulistiawati, Astri Fajriani. 2009. Type B Gelatin Gel Strength in Granule Formulation and its Mucoadhesive Characteristic. Jakarta, Indonesia: Syarif Hidayatullah University of Islam. MAKARA of Health Series, Vol 13, No 1.

Vitriana, 2002. Tuberculosis Spondylitis. Jakarta, Indonesia: Faculty of Medicine, University of Indonesia.

Warastuti Yessy and Basril Abbas 2011. Synthesis and Characterization of Irradiated Injectable Bone Substitute Paste Based on Hydroxyapatite. A Scientific Journal for The Applications of Isotopes and Radiation, Vol. 7, No. 2. BATAN Jakarta, Indonesia. ISSN 1907-0322.

Weiss Pierre, Olivier Gauthier, Jean-Michel Bouler, Gaël Grimandi, Guy Daculsi. 2007. Injectable Bone Substitute Using A Hydrophilic Polymer. France: Faculté de Chirurgie Dentaire.

WHO. Global Tuberculosis Control- Epidemiology, Strategy, Financing. WHO Report 2005. WHO/HTM/TB/2005.411.

Zuwanda and Raka Janitra. 2013. Diagnosis and Management of Tuberculosis Spondylitis. CDK-208, Vol. 40, No. 9.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Journal of Stem Cell Research and Tissue Engineering

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

View Stats

Creative Commons License
JSCRTE by Unair is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.