The Effect of Poly (Vynil Pyrrolidine) (PVP) Added in Variation of Ca2+ and PO43- Concentration in Microbial Cellulose-Hydroxyapatite Composite As Scaffold For Bone Healing

disca sandyakala

= http://dx.doi.org/10.20473/jscrte.v3i2.20358
Abstract views = 340 times | downloads = 149 times

Abstract


In Indonesia, the traffic accident causing 6 million people suffered injuries, particularly injuries fractures. 46.2% incidence of fractures occur in the lower extremities, 25% of them dying, 45% had a physical disability, 15% experienced psychological pressure and only 10% were healed well. Implant (graft) are used to support and accelerate the healing process of broken bones (bone healing). This study was done to make microbial cellulose-hydroxyapatite scaffold as a candidate for bone healing. Microbial cellulose obtained from culturing Acetobacter xylinum is used as a matrix and hydroxyapatite as a filler that is synthesized using the method of immersion in a solution of CaCl2 and KH2PO4, to increase the formation of apatite crystals, added polyvinyl Pyrrolidine (PVP). Scaffold synthesized using methods of freeze dried. Formation of composites varied in the concentration of Ca2+ and PO 3- of 25:125; 50:100; 75:75; 100:50 mM. The samples were then characterized using FTIR spectroscopy which shows the phosphate groups and the carbonate indicates the formation of hydroxyapatite in the eighth sample. Furthermore, to determine the morphology and identify the elements in the scaffold used SEM-EDAX, it was found that the pore formed measuring about 150-300 μm and obtained ratio of Ca / P best on microbial cellulose scaffold-PVP-hydroxyapatite with a variation of the concentration of Ca2+ and PO43- of 100 : 50 mM is equal to 0.6046 with an average degradation rate of 18.617% and the percentage of porosity contained in the sample amounted to 88.4%. This proves that microbial cellulose scaffold-PVP-hydroxyapatite with a variation of the concentration of Ca2+ and PO43- of 100:50 mM potential as a candidate for bone healing.


Keywords


bone healing, scaffold, selulosa mikrobial, hidroksiapatit, poly(vynil pyrrolidine), freeze dried

Full Text:

PDF

References


Al-Shamary, Elham Esmaeel., dan Amir Khalar

Al-Darwash. 2013. Influence of Fermentation Condition and Alkali Treatment on the Porosity and Thickness of Bacterial Cellulose Membranes. TOJSAT : The Online Journal of Science and Technology, Volume 3, Issue 2.

Alwiyah., Drs, Siswanto, M.Si., dan DR. Nurul Taufiqu Rochman, M.Eng. 2014. Pengaruh Variasi Magnesium Oxide (MgO) Terhadap Karakteristik Semen Gigi Modifikasi Nano Zinc Oxide Eugenol (ZOE). Jurnal Fisika dan Terapannya Vol. 2 No.1

Anam, Choirul., Sirojudin., dan Sofjan Firdausi. 2007. Analisis Gugus Fungsi Pada Sampel Uji, Bensin dan Spiritus Menggunakan Metode Spektroskopi FTIR. Berkala Fisika, Vol 10, No.1, Universitas Diponegoro.

Ary Andini., Dyah Hikmawati., dan Sri Sumarsih. 2014. Potensi Kolagen Kulit Ikan Lele Sangkuriang (Clarias gariepinusvar) Sebagai Scaffold Kolagen-Hidroksiapatit pada Bone Tissue Engineering. Jurnal Fisika dan Terapannya Vol. 2 No.1

Bankoff, A.D.P. 2007. Morfologia e Cinesiologia Aplicada ao Movimento Humano. Editora Guanabara Koogan, Rio de Janeiro-Brasil.

Barralet, J.E., Wang, L., Lawson, M., Triffit, J.T., Cooper, P.R., dan Shelton,

R.M. 2005. Comparison of Bone Marrow Cell Growth on 2D and 3D Alginate Hydrogels. J, Mater Sci Mater Med 16(6):515-519.

Bielecki, S., Krystoynowicz, A., Turkiewicz, M., dan Kalinowska, H. Bacterial Cellulose. Biopolymers : Vol 5, Polysaccharides I, Polysaccharides from Prokaryotes. Weinham: Wiley, p37-46.

Black, J.M., dan Hawks, J.H. 2009. Medical Surgical Nursing : Clinical Management for Positive outcome, 8thed. St. Louis Missouri : Elsevier Saunders.

Bohner, M., dan Lemaitre, J. 2009. Can Bioactivity be Tested In Vivo with SBF Solution. Biomaterials. 30 (12), 2175-2179.

Bucholz, R.W., Heckman, J.D., Court-Brown, C.M., dan Green. 2006. Fractures in Adults, 6thed. USA: Maryland Composition. p80-331

Burchardt, H. 1978. Transplantation of Bone. Surg Vlin North Am 58:403-427

Chang, R.W., Hansen, P., dan Kraner, E.J. 1992. Material Science And Technology (Comprehensive Treatment) 14.

Clarke, B. 2008. Normal Bone Anatomy and Physiology. Clin J Am Soc Nephrol 3:S131-S139.

Charles Sfeir, et al. 2009. Bone Regeneration and Repair : Biology and Clinical Applications. Humana Press Inc, Totowa, NJ.

Darmawan, Darwis., dan Yessy Warastuti. 2008. Sintesis dan Karakterisasi Komposit Hidroksiapatit (HA) Sebagai Graft Tulang Sintetik. Jurnal Ilmiah Aplikasi Isotop dan Radiasi, Vol. 4 No. 2.

Darmawan, Darwis. 2009. Effect of Gamma Irradiation On Microbial Cellulose Membrane For Application In Guided Bone Regeneration (GBR). Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol. 2 No. 1.

Depkes R.I. 2007. Riset Kesehatan Dasar. Diunduh pada 15 Desember 2014. http://www.depkes.co.id.

Djoko Simbardjo Iskandar. 2008. Usaha Mandiri di Bidang Orthopaedi Khusus Seksi Traumatologi dan Rekonstruksi di Era Globalisasi. Majalah Kedokteran Indonesia, Vol. 58 No. 3.

Eslami, et al. 2008. Synthesis and Characterization of Hydroxyapatite Nanocrystals cia Chemical Precipitation Technique. Iranian Journal of Pharmaceutical Sciences Spring 4(2): 127-134.Fabia K. Andrade., Nuno Alexandre., Irina Amorim., Fatima Gartner., Ana Collete Mauricio., Ana Lucia Luis., dan Miguel Gama. 2012. Studies on The Biocompability of Bacterial Cellulose. SAGE : Jpurnal of Bioactive and Compatible Polymers, p1-17.

Fernando, G. Torres., Solene, Commeaux., dan Omar, P. Tronsoco. 2012. Biocompability of Bacterial Cellulose Based Biomaterials. Journal of Functional Biomaterials 3; p864-878.

Flottmann., Hubertus., dan Quadir Anisul. 2008. Polyvynil pyrrolidone (PVP) – One of The Most Widely Used Exipients in The Pharmaceuticals: An Overview. Drug Delivery Technology.

Helmi, Z.N. 2011. Buku Ajar Gangguan Muskuloskeletal. Jakarta: Salemba Medika. P411-55.

Hutchens, S.A., Woodward, J., Evans, B.R., dan O’neil, H.M. 2004. Composite Material. United States Patent 20040096506, May 20, 2004.

Istifarah., Aminatun., dan Prihartini Widiyanti. 2012. Sintesis dan Karakteristik Komposit Hidroksiapatit dari Tulang Sotong (Sepia sp.)-Kitosan untuk Kandidat Aplikasi Bone Filler. Program Studi Fisika Fakultas Sains dan Teknologi Universitas Airlangga.

Jones, S.A., Martin, G.P., Royall, P.G., dan Brown, M.B. 2005. Biocompatible polymer blends :Effects of Physical Processing on The Molecular Interaction of Poly(vynil Alcohol) and Poly(Vynil Pyrrolidine). Jpurnal of Applied Polymer Science, Vol. 98, No. 5, pp2290-2299.

Junping Wang., Yizhou Zhu., dan Jian Du. 2011. Bacterial Cellulose : A Natural Nanomaterial For Medical Applications. World Scientific: Journal of Mechanics in Medicine and Biology, Vol. 11, No. 2, p285-306.

Karageorgiou, V., dan D Kaplan. 2005. Porosity of 3D Scaffolds and Osteogenesis. Biomaterials, 26, 5474-5491.

Kawai, T., Ohtsuki, C., Kamita, M., Miyazaki, T., Tanihara, M., Sakaguchi, Y. 2004. Coating on an Apatite Layer on Polyamide Films Containing Sulfonic Groups by Biomimetic Process. Biomaterials, 25, 4529-4534.

Kuznetsova, D. S., P. S. Timashev., V.N. Bagratashvili., dan E.V. Zagaynova. 2014. Scaffold And Cell System-Based Bone Grafts In Tissue Engineering (Review).

Marion Pommet., Julasak Juntaro., Jerry Y.Y. Heng., Athanosios Mentalaris., Adam F. Lee., Karen Wilson., Gerhard Kalinka., Milo S.P. Shaffer., dan Alexander Bismarck. 2008. Surface modification of Natural Fibers Using Bacteria L Depositing Bacterial Cellulose on to Matural Fibers to Create Hierarchical Fiber Reinforced Nanocomposites. American Chemical Society: Biomacromolecules, 9 p1643-1651.

M. Doblare., J.M. Garcia., dan M.J. Gomez. 2004. Modelling Bone Tissue Fracture and Healing : A Review. Engineering Fracture Mechanics 71:1809-1840.

Miao, Xigeng., dan Dan Sun. 2010. Graded/ Gradient Porous Biomaterials. Materials, 3, 26-47.

Muller, L., dan Frank, AM. 2006. Preparation of SBF with Different Content and Its Influence On The Compositon of Biomimetic Apatites, Acta Biomaterialia. 2(2), 181-189.

Petrovic, M., B. Colovic., V. Jokanovic., dan Dejan Markovic. 2012. Self Assembly of Biomimetic Hydroxyapatite on The Surface of Different Polymers Thin Films. Journal of Ceramic Processing Research, Vol 13, No. 4, pp 398-404.

Recker, R.R. 1992. Disorders od Bone and Mineral Metabolism. Embryology, anatomy, and microstructur of bone in Coe FI, Favus MJ (eds). New York : Raven. pp 219-240

Rozita, A.R., Rohana Adnan., Mohammad A.B., dan Sam’an M.M. 2011. Synthesis and Characterisation of Pure Nanoporous Hydroxiapatite. Jurnal of Physical Science, Vol. 22(1), 25-37.

Salter, R.B. 1999. Textbook Disorders and Injuries of The Muskuloskeletal System, 3rded. USA: Lippincott Williams and Wilkins. P417-498.

Saska, S., H. S. Barud., A. M. M. Gaspar., R. Marchetto., S. J. L. Riberio., dan Y. Messaddeq. 2011. Bacterial Cellulose - Hydroxyapatite Nanocomposites for Bone Regeneration. Internationals Journal of Biomaterials.

Sianturi, Josafat. 2008. Preparasi dan Karakterisasi Kalsium Ferrite (CeFe4o7) untuk Indentifikasi Sel Kanker Melalui Simulated Body Fluid (SBF) Kokubo. Institut Teknologi Bandung: Bandung.Smeltzer. S., dan Bare, B. 2009. Brunner and Suddarth’s : Text Book Medical Surgical Nursing. St. Louis Missouri : Elsevier Saunders.

Srivastava, Arun., dan Jain, Vinod K. 2012. Applying SEM-EDX and XRD Technique to Demonstrate the Overgrowth of Atmospheric Soot and Its Coalescence with Crystal Silicate Particles in Delhi. Atmospheric and Climate Sciences,2, 89-93.

Suseno, Jatmiko Endro., dan K. Sofjan Firdausi. 2008 Rancang Bangun Spektroskopi FTIR (Fourier Transform Infra Red) untuk Penentuan Kualitas Susu Sapi. Berkala Fisika, Vol. 11, hal 23-28, Universitas Diponegoro.

Syafrudin, H. 2011. Analisis Mikrostruktur, Sifat Fisis dan Sifat Mekanik Keramik Jenis Refraktori. Skripsi Departemen Fisika, Fakultas Sains dan Teknologi, Universitas Airlangga Surabaya.

Taichman, R.S. 2005. Blood and Bone : Two Tissues whose Fates Are Inertwined to Create the Hematopoietic Stem Cell Niche. Blood 105:2631-2639

Windarti, Tri., dan Yayuk Astuti. 2006. Pengaruh Konsentrasi Ca2+ dan PO43- Pada Pembentukan Hidroksiapatit di Dalam Matriks Selulosa Bakterial. Kimia FMIPA Universitas Diponegoro.

Usha, Kini., dan B.N., Nandeesh. 2012. Physiolofy of Bone Formation, Remodeling, and Metabolism. Spinger-Verlag Berlin Heidelberg.

Wang, Qiaanbin., Qiguang Wang., dan Changxiu Wan. 2011. The Effect of Porosity on The Structure And Properties of Calcium Polyphospate Bioceramics. Ceramics, Silikaty 55 (1) 43-48.

Warastuti, Yessy., dan Basril Abbas. 2011. Sintesis dan Karakterisasi Pasta Injectable Bone Substitute Iradiasi Berbasis Hidroksiapatit. Jurnal Ilmiah Aplikasi Isotop dan Radiasi, ISSN 1907-0322.

Warastuti, Yessy., dan Suryani, Nani. 2013. Karakteristik Degradasi dari Biomaterial Poli-(kaprolakton- kitosan-hidroksiapatit) Iradiasi dalam Larutan Simulated Body Fluid. Pusat Aplikasi Teknologi Isotop dan Radiasi, BATAN: Jakarta Selatan.

Williams, P.L. 1995. Gray’s Anatomy 38th ed. Churchill Livingstone.

WHO. 2011. Decade of Action on Road Safety : Indonesia. Diakses pada 15 Desember 2014. www.who.searo/int.

Yamanaka et al. 1989. J. Mat. Sci 24:3141- 3145.

Yamane, C., Okajima, K., dan Otsuka, M. 2004. Novel Cellulose-type Material. United State Patent 20040267006, Desember 30.

Yin, Na., et al. 2011. Biomimetic Mineraliation Synthetis of Hydroxyapatite Bacterial Cellulose Nanocomposite. Donghua University: Shanghai.

Zhu, Ning and Chen, Xiongbiao. 2013. Biofabrication of Tissue Scaffolds. InTech: Advances in Biomaterials Science and Biomedical Applications.


Refbacks

  • There are currently no refbacks.


Copyright (c) Journal of Stem Cell Research and Tissue Engineering

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

View Stats

Creative Commons License
JSCRTE by Unair is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.