The effect of Extreme Low Frequency-Pulsed Electromagnetic Field exposure in the healing process of Sprague Dawley mouse delayed union femur fracture: study of Rust Radiology Score

andika djaja

= http://dx.doi.org/10.20473/jscrte.v4i1.21589
Abstract views = 242 times | downloads = 195 times

Abstract


Fractures usually heal normally. In some conditions, the healing process do not occur normally, but become delayed union or non union as a complication. Successful healing of fractures is a complex interaction between the process of angiogenesis and osteogenesis (the interaction of osteoblasts and osteoclast). Physical stimuli such as exposure of EMF (electromagnetic fields) influences of the osteogenesis process both in the development stage of embryo reinforcement and the fracture healing stage. The aim of this study is to determine the healing of delayed union fractures in experimental animals due to the expossure of Extreme Low Frequency-Pulsed Electromagnetic Field (ELF-EMF) by comparing the RUST scores. The experimental study was conducted Department of Nutrition Animal Laboratory, Faculty of Medicine, Universitas Indonesia with 56 experimental rats during August-September 2018. There were no differences of animal characteristics in the study. It was found that there were significant differences in Rust Score in the treatment and control groups in each examination week. There were no differences in clinical improvement in the two groups.  This study concluded that there was an improvement in delayed union fracture healing after the administration of ELF-EMF as seen from the difference in Rust score.


Keywords


Extreme Low Frequency–Pulsed Electromagnetic Field, delayed union fracture, Rust score

Full Text:

PDF

References


Aaron RK, Ciombor DM, and Simon BJ. Treatment of Nonunions with Electric and Electromagnetic Fields. 2004 Clin Orthop Relat Res 419 21-29.

Assiotis A, Sachinis NP, and Chalidis BE. Pulsed electromagnetic fields for the treatment of tibial delayed unions and nonunions. A prospective clinical study and review of the literature. 2012 J Orthop Surg Res 7 24.

Bilezikian J and LawrenceGR. Rodan GA (ed) Principles of Bone Biology. 2nd ed. 1996. Portland: Academic Press.

Brinker MR and O'Connor DP. The incidence of fractures and dislocations referred for orthopaedic services in a capitated population. 2004 J Bone Joint Surg Am 86-A 290-297.

Çekic E and Alici E. Verification of the reliability of the Radiographic Union Score for Tibial Fractures in the follow-up of patients with tibia corpus fractures operated via the intramedullary nailing technique using the patients' clinical condition. 2013 Injury 44 S8.

Einhorn TA and Gerstenfeld LC. Fracture healing: mechanism and interventions. 2015 Nat Rev Rheumatol 11 45-54.

Fu YC, Lin CC, Chang JK, Chen CH, Tai IC, Wang JW, et al. A novel single pulsed electromagnetic field stimulates osteogenesis of bone marrow mesenchymal stem cells and bone repair. 2014 PloS One 9 e91581.

Ganveer GB and Tiwari RR. Injury pattern among non-fatal road traffic accident cases: a cross-sectional study in Central India. 2005 Indian J Med Sci 59 9-12.

Gao F, Lv TR, Zhou JC, and Qin XD. Effects of obesity on the healing of bone fracture in mice. 2018 Journal of Orthopaedic Surgery and Research 13 145.

Giannoudis PV, Einhorn TA, and Marsh D. Fracture healing: the diamond concept. 2007 Injury 38 S3-S6.

Giannoudis PV, Einhorn TA, Schmidmaier D, and Marsh D. The diamond concept- open questions. 2008 Injury 39 S5- S8.

Gossling HR, Bernstein RA, and Abbott J. Treatment of ununited tibial fractures: A comparison of surgery and pulsed electromagnetic fields (PEMF). 1992 Orthopedics 15 711–719.

Hammer R, Hammerby S, and Lindholm B. Accuracy of radiologic assessment of tibial shaft fracture union in humans. 1985 Clin Orthop Relat Res 199 233- 238.

Kooistra BW, Dijkman BG, Busse JW, Sprague S, Schemitsch EH, and Bhandari M. The Radiographic Union Scale in Tibial Fractures: Reliability and Validity. 2010 J Orthop Trauma 24 S81-S86.

Lu C, Miclau T, Hu D, and Marcucio RS. Ischemia leads to delayed-union during fracture healing: a mouse model. 2007 J Orthop Res 25 51-61.

Marcelini S, Henriquez JP, and Bertin A. Control of osteogenesis by the canonical Wnt and BMP pathway in vivo. 2012 Bioessay 34 953-962.

Mooney MP and Siegel MI. Animal models for bone tissue engineering of critical- sized defects (CSDs), bone pathologies, and orthopedic disease states. In: Hollinge JO, Einhorn TA, Doll BA, Sfeir C, editors. Bone tissue engineering. 2005. Boca raton: CRC Press. 217-244.

Ongaro A, Pellati A, Bagheri L, Fortini C, Setti S, and De Mattei M. Pulsed Electromagnetic Fields Stimulate Osteogenic Differentiation in Human Bone Marrow and Adipose Tissue Derived Mesenchymal Stem Cells. 2014 Bioelectromagnetics 35 426- 436.

Phieffer LS and Goulet JA. Delayed unions of the tibia. 2006 Instr Course Lect 55 389-401.

Riyadina W, Suhardi, and Permana M. Pola dan Determinan Sosiodemografi Cedera Akibat Kecelakaan Lalu Lintas di Indonesia. 2009 Maj Kedokt Indon 59 464-472.

Shi HF, Xiong J, Chen YX, Wang FJ, Qiu XS, Wang YH, et al. Early application of pulsed electromagnetic field in the treatment of postoperative delayed union of long-bone fractures: a prospective randomized controlled study. 2013 BMC Musculoskeletal Disord. 14 35.

Shirley D, Marsh D, Jordan G, McQuaid S, and Li G. Systemic recruitment of osteoblastic cells in fracture healing. 2005 J Orthop Res 23 1012-1021.

Singer BR, McLauchan GJ, Robinson CM, and Christie J. Epidemiology fractures in 15.000 adults : the influence of age and gender. 1998 J Bone Joint Surg (Br). 80 243-248.

Teven CM, Greives M, Natale RB, Su Y, Luo Q, He BC, et al. Differentiation of osteoprogenitor cells is induced by high frequency pulsed electromagnetic field. 2012 J Craniofac Surg 23 586-593.

Utvåg SE, Grundnes O, Reikeras O. Effects of periosteal stripping on healing of segmental fractures in rats. 1996 J Orthop Trauma 10 279-284.

Victoria G, Petrisor B, Drew B, and Dick D. Bone stimulation for fracture healing: What’s all the fuss? 2009 Indian J Orthop 43 117-121


Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Journal of Stem Cell Research and Tissue Engineering

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

View Stats

Creative Commons License
JSCRTE by Unair is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.