Genetic Stability The Protein Encoding Envelope (E) Genes Dengue Virus Serotype-4 Passaged in Vero Cell As A Candidate Chimera Vaccine Material

deya karsari

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This study aims to  analyze   genetic stability of  the gene encoding the envelope protein (E) dengue virus serotype-4 passaged in vero cells, Denv-4 passaged  in vero cells serially then continued with RNA extraction at passage 0, 10, 20, 30, 40 ,50 , and 60, and then continued with two step PCR and amplification, and sequencing then analyze the nucleotide stability with BLAST and MEGA 5 software. The result shows that there are many variable site in nucleotide and amino acid with high mutation rate 57.4% for nucleotide and 71.9% for amino acid ,while the similarity between passages are high ranging from 91% - 98%. The conclusion for this study is Denv-4 after analyzed shows that the gene encoding protein E has many variable site but high in similarity.


Dengue virus serotype-4, envelope, passage, stability

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Blaney, J.E.Jr., G. G. Manipon, C.Y. Firestone, D. H. Johnson,C. T. Hanson, B. R. Murphy, S. S. Whitehead. 2003. Mutations Which Enhance The Replication of Dengue Virus Type 4 and An Antigenic Chimeric Dengue Virus Type 2/4 Vaccine Candidate in Vero cells. Vaccine 21: 4317–4327

Chi-Lee, H., M. Butler, S.C. Wu. 2012. Using recombinant DNA technology for the development of live-attenuated dengue vaccines. Enzyme and Microbial Technology 51: 67– 72

Coller, B.A.G., D.E. Clements, A.J. Bett, S.L. Sagar, J.H.T. Meulen. 2011. The development of recombinant subunit envelope-based vaccines to protect against dengue virus induced disease. Vaccine 29: 7267– 7275

Kenney J. L., S. M. Volk, J. Pandya, E. Wang, X. Liang, and S. C. Weaver. 2011. Stability of RNA Virus Attenuation Approaches. National of Healh Public Access University of Texas. Vaccine 29: 2230–2234

Klungthong, C., C.Zhang, M.P. Mammen Jr, S. Ubol and E.C. Holmes. 2004. The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand. Virology 329:168–179

Kuiken, C. And J. Turmond. 2005. Genomic Map for Dengue Virus 4. HFV sequence data base. bin/HFV/GenomeMapper

Kuhn, R.J., W.Zhang, M.G.Rossman, S.V. Pletnev, J.Corver, E.Lenches, C.T., Jones, S.Mukhopadhyay, Chipman P.R. and Strauss J.H .2002. Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell. 108:717–725

Liu C.C., S.C. Lee, M. Butler, S.C. Wu. 2008. High Genetic Stability of Dengue Virus Propagated in MRC-5 Cells as Compared to the Virus Propagated in Vero Cells. Plosone Volume 3 , Issue 3, e1810.

Mantel N., Y. Girerd, C. Geny, I. Bernard, J. Pontvianne, J. Lang, V. Barban. 2011. Genetic Stability of a Dengue Vaccine Based on Chimeric Yellow Fever/Dengue Viruses. Vaccine 29: 6629– 6635

Nor Y. A., N. H. Sulong, M. Mel1, H. M. Salleh, I. Sopyan. 2010. The Growth Study of Vero Cells in Different Type of Microcarrier. Materials Sciences and Applications, 1: 261-266

Sasmono, T., B. Yohan, T.Y. Setianingsih, Aryati, P. Wardhani dan F.A. Rantam. 2012. Identifikasi Genotipe dan Karakterisasi Genome Virus Dengue di Indonesia untuk Penentuan Prototipe Virus Bahan pembuatan Vaksin Dengue Berbasis Strain Indonesia. RD-2012-0422.htm

World Health Organization. Vector-borne viral infections: dengue fever [online]; 2009 eases/vector/en/index1.html.


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