The Role of adaptive MSCs as an Attempt Regeneration of Spermatogenesis Process Using by Hypoxia Precondition In Vitro
Downloads
The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn't experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did'nt experience become progenitor cells was not expectation (still undifferentiated).
Blanchard K.T., J. Lee, and K. Boekelheide. 1998. Leuprolide, a Gonadotropin- Releasing Hormone Agonist, Reestablishes Spermatogenesis After 2,5- Hexanedione-Induced Irreversible Tsticular Injury in the Rat, Resuting in Normalized Stem cell Factor Expression. Endocriology.139 (1): 236-244.
Cavallini G. 2006. Male idiopathic oligoasthenoteratozoospermia. Asian J. Androl., 8(2): 143-157.
Hamanaka RB and NS Chandel. 2010. Mitochondrial Reactive Oxygen Species Regulate Cellular Signaling and Dictate Biological Outcomes Trends Biochem. Sci., 35: 505-513.
Kenichiro H., N. Noritoshi, I. Takashi, T. Itoh., S. Murakami, Y. Shimizu,W. Taki, K. Miyatake,K. Kangawa. 2005. Adrenomedullin Enhances Therapeutic Potency of Mesenchymal Stem Cells after Experimental Stroke in Rats. Stroke36: 853-858.
Rantam FA, Ferdiansyah, Nasronudin and Purwati. 2009. Stem Cell Exploration- Methods of lsolation and Culture First Ed. Airlangga University Press. Surabaya.
Suda T, Takubo Kand Semenza GL. 2011. Cell Stem Cell Review. Metabolic Regulation of ematopoietic Stem Cells in Hypoxic Niche. Cell Stem Cell., 9: 296-310.
Suzuki K, Murtuza B and Beauchamp JR. 2004. Dynamics and Mediators of Acute Graft Attrition After Myoblast Transplantation to the Heart. FASEBJ., 18: 1153-1155.
Takubo K, Goda N, Yamada W, Iriuchishima H, Ikeda E, Kubota Y, Haruko S, Jhonson RS, Hirao A, SuematsuM and Suda T.2010. Regulation of the HIF-1 Level is Essentialfor Hematopoietic Stem Cells. Cell Stem Cell., 7: 391-402.
Tang Y.L., Y.C. Zhang, L.P. Qian, Shen, and M. Phillips (2005). Improved Graft Mesenchymal Stem Cell Survival in Ischemic Heart with a Hypoxia regulated Heoxygenase 1 Vector. J. Am. Coll. Cardiol.46: 1339-1350.
1. As an author you (or your employer or institution) may do the following:
- make copies (print or electronic) of the article for your own personal use, including for your own classroom teaching use;
- make copies and distribute such copies (including through e-mail) of the article to research colleagues, for the personal use by such colleagues (but not commercially or systematically, e.g. via an e-mail list or list server);
- present the article at a meeting or conference and to distribute copies of the article to the delegates attending such meeting;
- for your employer, if the article is a ‘work for hire', made within the scope of your employment, your employer may use all or part of the information in the article for other intra-company use (e.g. training);
- retain patent and trademark rights and rights to any process, procedure, or article of manufacture described in the article;
- include the article in full or in part in a thesis or dissertation (provided that this is not to be published commercially);
- use the article or any part thereof in a printed compilation of your works, such as collected writings or lecture notes (subsequent to publication of the article in the journal); and prepare other derivative works, to extend the article into book-length form, or to otherwise re-use portions or excerpts in other works, with full acknowledgement of its original publication in the journal;
- may reproduce or authorize others to reproduce the article, material extracted from the article, or derivative works for the author's personal use or for company use, provided that the source and the copyright notice are indicated, the copies are not used in any way that implies JSCRTE endorsement of a product or service of any employer, and the copies themselves are not offered for sale.
All copies, print or electronic, or other use of the paper or article must include the appropriate bibliographic citation for the article's publication in the journal.
2. Requests from third parties
Although authors are permitted to re-use all or portions of the article in other works, this does not include granting third-party requests for reprinting, republishing, or other types of re-use. Requests for all uses not included above, including the authorization of third parties to reproduce or otherwise use all or part of the article (including figures and tables), should be referred to JSCRTE by going to our website at http://e-journal.unair.ac.id/index.php/JSCRTE
3. Author Online Use
- Personal Servers. Authors and/or their employers shall have the right to post the accepted version of articles pre-print version of the article, or revised personal version of the final text of the article (to reflect changes made in the peer review and editing process) on their own personal servers or the servers of their institutions or employers without permission from JSCRTE, provided that the posted version includes a prominently displayed JSCRTE copyright notice and, when published, a full citation to the original publication, including a link to the article abstract in the journal homepage. Authors shall not post the final, published versions of their papers;
- Classroom or Internal Training Use. An author is expressly permitted to post any portion of the accepted version of his/her own articles on the author's personal web site or the servers of the author's institution or company in connection with the author's teaching, training, or work responsibilities, provided that the appropriate copyright, credit, and reuse notices appear prominently with the posted material. Examples of permitted uses are lecture materials, course packs, e-reserves, conference presentations, or in-house training courses;
- Electronic Preprints. Before submitting an article to an JSCRTE, authors frequently post their manuscripts to their own web site, their employer's site, or to another server that invites constructive comment from colleagues. Upon submission of an article to JSCRTE, an author is required to transfer copyright in the article to JSCRTE, and the author must update any previously posted version of the article with a prominently displayed JSCRTE copyright notice. Upon publication of an article by the JSCRTE, the author must replace any previously posted electronic versions of the article with either (1) the full citation to the work with a Digital Object Identifier (DOI) or link to the article abstract in JSCRTE homepage, or (2) the accepted version only (not the final, published version), including the JSCRTE copyright notice and full citation, with a link to the final, published article in journal homepage.
4. Articles in Press (AiP) service
JSCRTE may choose to publish an abstract or portions of the paper before we publish it in the journal. Please contact our Production department immediately if you do not want us to make any such prior publication for any reason, including disclosure of a patentable invention.
5. Author/Employer Rights
If you are employed and prepared the article on a subject within the scope of your employment, the copyright in the article belongs to your employer as a work-for-hire. In that case, JSCRTE assumes that when you sign this Form, you are authorized to do so by your employer and that your employer has consented to the transfer of copyright, to the representation and warranty of publication rights, and to all other terms and conditions of this Form. If such authorization and consent has not been given to you, an authorized representative of your employer should sign this Form as the Author.
6. JSCRTE Copyright Ownership
It is the formal policy of JSCRTE to own the copyrights to all copyrightable material in its technical publications and to the individual contributions contained therein, in order to protect the interests of the JSCRTE, its authors and their employers, and, at the same time, to facilitate the appropriate re-use of this material by others. JSCRTE distributes its technical publications throughout the world and does so by various means such as hard copy, microfiche, microfilm, and electronic media. It also abstracts and may translate its publications, and articles contained therein, for inclusion in various compendiums, collective works, databases and similar publications
Every accepted manuscript should be accompanied by "Copyright Transfer Agreement" prior to the article publication.
