The Therapy of Burn Wound Healing in Rat (Wistar) by Using the Combination of Peripheral Blood Mononuclear Cells (PBMCs) and Bone Marroe BM-Derived Mesenchymal Stem Cell

Gusti Revilla

Abstract views = 351 times | downloads = 270 times


The therapy to heal the bum wound is still imperfect, therefore it is important to conduct specific research concerning this topic that benefits to society. Using both Bone Marrow-stem cell (BM-MSc) and peripheral blood mononuclear cells (PBMCs) from allogenic donors as part of the therapy to heal the bum wound seems to give positive prospect for the future treatment. In this experiment, PBMC and rat BM- derived from mesenchymal stem cell were used as the therapy model. lmmunocytochemistry was used as the method to characterize the phenotype of MSc, It was also used to express the collagen type I and the Indirect ELISA in analyzing the TGF·P 1 secretion.  The rats with bum wound were divided into 2 kinds of group; the first group of rats was selected to control the use of PBS; while second group of rats was used as the treatment object that was medicated by the applying of the combination of both BM-MSC and PBMC. Stem-cells subcutaneously administered dose applied to each rat was around of 2 x 106 cells. The result showed that the levels of TGF-β1 secretion in day 3rd and day 7th on the rats which were treated by using the combination of BM-MSC and PBMC were higher compared to the rats from the control group.  The experiment that concerns on the thickness of the collagen showed that combination between BM-MSC and PBMC stem cell make it possible in increasing the thickness of collagen 1, besides; it also showed significant differences (p=0.000). This research proved that the combination of BM-MSC and PBMC stem cell served can accelerate the healing process for the bum wound on rats through Increasing of TGF-P 1 secretion and collagen type 1 expression, It means that PBMCs can be applied as good as chemoattractant.


Wound healing, stem cell, allogenic, TGF-β1, type 1 collagen.

Full Text:



Abe R, Donnely SC, Peng T, Bucala R, Metz CN. 2001. Peripheral blood fibrocytes: differentiation pathway and migration to wound sites. J. lmmunol. (166): pp. 7556-62.

Evers LH, Bhavar D, Malla.nder P. 2010. The biology of bum injury. Experi. Dermatol. (19): pp. 777-783.

Herdrich BJ, Lind RC, Liechty KW. 2008. REVIEW Multi potent adult progenitor cells: their role in wound healing and the treatment of dermal wounds. Cytotherapy10 (6): pp. 543-550.

Kumar S, Peng FW, David JL. 2004. What is new in wound healing. Turkish J. Med. Sci. (34): pp. 147-160.

Lau K, Paus R, Tiede S, Oay P, Bayat A. 2009. Exploring the role of stem cells in cutaneous wound healing. Experi. Dermatol. (18): pp. 921-933.

Liu ZJ, Zhuge Y, Velazquez OC. 2008. Trafficking and differentiation of mesenchymal stem cells. J. Cellul. Biochem. (106): pp. 984-991.

Maxson S, Lopez EA, Yoo D, Miagkova AD, Leroux MA. 2012. Concise Review: Role of mesenchymal stem cells in wound repair. Stem cells translation medicine; (1): pp. 142-149.

Medina A, Erin B, Nicholas C, Aziz G. 2009. Circulating monocytes have the capacity to be trans-differentiated into keratinocyte-like cells. Wound repair and regeneration 17, pp. 268-277 by the Wound Healing Society.

Metcalfe AD, Ferguson MWJ. 2007. Tissue engineering of replacement skin: the crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration. J. Royal Soc. Interface. (4): pp. 413-437.

Moioli EK, Clark PA, Chen M, Dennis JE, Erickson HP, Gerson SL and Mao JJ. 2008. Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing Bioengineered Tissues. Plos One 3 (12): pp.1-11.

Newman SL, Tucci MA. 1990. Regulation of human monocyte/macrophage function by extracellular matrix adherence of monocytes to collagen matrices enhances phagocytosis of opsonized bacteria by activation of complement receptors and enhancement of Fc receptor function. J. Clin. lnvestig. 86, pp. 703-714.

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti OW, Craig S, Marshak OR. 1999. Multi-lineage potential of adult human mesenchymal stem cells. Science (284): pp. 143--147.

Sasaki M, R Abe, Y Fujita, S Ando, D lnokuma, Hiroshi Shimizu. 2008. Mesenchymal stem cells are recruited into skin cell type repair by trans-differentiation into multiple wounded skin and contribute to wound. J. lmmunol. 2008; (180): pp. 2581-2587.

Smith AN, Willis E, Chan VT, Muffley LA, lsik FF,Gibran NS, Hocking AM. 2010. Mesenchymal stem cells induce dermal fibroblast responses to injury Experimental Cell Research. January 1; 316(1 ): pp. 48-54.

Syamsuhidayat R. 1997. Buku ajar ilmu bedah. Edisi Revisi, Penerbit Buku Kedokteran EGC.Jakarta Vem AK, BA Latense. 2001. Specimen collection and analysis bum wound. Methods in molecular medicine wound healing. Edited by Luisa D & Aime LB. 78. Human Press Inc.

Totowa NJ, Yang L, Chan T, Demare J, lwashina T, Ghahary A, Scott PG, Tredget EE. 2001. Healing of Bum Wounds in Transgenic Mice Overexpressing Transforming Growth Factor-β1 in the Epidermis. Ameri. J. Pathol. 159 (6), Yang L, Qiu ex, Ludlow A Ferguson MWJ, Brunner. 1999. Technical advance active transforming growth factor-b in wound repair determination using a new assay. Am. J. Pathol. (154): pp. 10&-111.


  • There are currently no refbacks.

Copyright (c) 2020 Journal of Stem Cell Research and Tissue Engineering

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

View Stats

Creative Commons License
JSCRTE by Unair is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.