COMPARISON IN CORNEAL COLLAGEN STRUCTURE BETWEEN 20% PLATELET RICH PLASMA AND 20% AUTOLOGOUS SERUM THERAPY POST NATRIUM HIDROKSIDA EXPOSURE
Experimental study on Oryctolagus Cuniculus
Downloads
Objective to compare therapy response between 20% PRP and AS 20% on cornea post sodium hydroxide exposure on collagen structure. Methods this is a true experimental study with post test only design on 18 New Zealand white rabbits. Sample were divided randomly to two groups, each with 9 rabbits. The right eye of each rabbit were exposed to alkali injury with 1N NaOH under general anesthesia. First group was given 20% PRP eyedrops and 20% AS for the second group. And the end of the 7 day periode, all rabbits were euthanized and enucleated to obtain histopthological data. Collagen density, collagen thickness and keratosit cell were evaluated. The result will be analyzed, ratio scale data will be tested with independent T- test, and ordinal scaled data will be tested with Mann-Whitney test. Result The collagen density in the 20% PRP group shows 88.9% grade 2 and 44.4 % grade 2 for the 20% AS group. The collagen thickness in the 20% PRP group is 55.41 and 67.62 for the 20% AS group. The keratosit cell count in the 20% PRP group are 54.56 and 45.47 in the 20% AS group. There is significant difference in the collagen density between 20% PRP and 20% AS treatment (p= 0.066). There is significant difference in the collagen thickness between 20% PRP and 20% AS treatment (p= 0,224). There is significant difference in the keratosit cell count between 20% PRP and 20% AS treatment (p= 0,227). Conclusion From the statistik analysis shows that there is no significant difference in corneal collagen structure between 20% PRP and 20% AS therapy post natrium hidroksida exposure, from our study there is no significant better corneal healing parameters in post alkali chemical ocular injury with 20% PRP eyedrops compare to 20% AS eyedrops.
Anitua E, Muruzabal F, Alcalde I, Merayo-Lloves J & Orive G, 2013. Plasma rich in growth factors (PRGF-Endoret) stimulates corneal wound healing and reduces haze formation after PRK surgery, Experimental Eye Research, Elsevier Journal, vol. 115, p.153-161.
Damayanti SA, Yuwono B, Dwi, & Robin dwi MC, 2012. Efek Pemberian Kurkumin terhadap Peningkatan Pembentukan Kolagen pada soket gigi tikus wistar pasca pencabutan, tesis. p. 1-4.
Geerling G, Maclennan S & Hartwig D, 2004. Autologous Serum Eye Drops for Ocular Surface Disorders, Br J Ophthalmol, vol.88, no.11, p. 1467 – 1474.
Hassell RJ & Birk DE, 2010. The molecular basis of corneal transparency. Elsevier journal. Experimental Eye Research 91. p 326- 335.
Kaur M, Sinha R & Sharma N, 2014. Acute Chemical Injuries. DOS Times.Vol 9, no 9, p.41-45.
Khaskar E, Aldavood SJ, Abedi GR, Sedaghat R, Nekoui O & Zamani ahmadmahmudi M, 2013. The Effect of Sub-Conjungtival Platelet Rich Plasma in Combination With Topical Acetylcysteine on Corneal Alkali Burn Ulcer In Rabbits, Comparative Clinical Pathology, Jan, vol.22, no.1, p. 107- 112.
Kim KM, Shin YT & Kim HY, 2012 Effect of Autologous Platelet-Rich Plasma On Persistent Corneal Epithelial Defect After Infectious Keratitis, Jpn J Ophthalmol, vol.56, no.6, p. 544 – 550.
Lekhanont K, Jongkhajornpong P, Choubtum L & Chuckpaiwong V, 2013. Topical 100 % Serum Eye Drops for Treating Corneal Epithelial Defect after Ocular Surgery, BioMed Research International, vol, artikel ID. 521315, p.7.
Luo HL, Lu YB, Wu T, Zhang M, Zhana Y& Jin Y, 2013. Construction of Tissue Engginered Cornea Composed of Amniotic Epithelial Cell anda Acellular Porcine Cornea for Treating Coneal alkali Burn, Biomaterials journal, vol 34, p.6748-6759.
Piccin A, Di Pierro AM, Canzian L, Primerano M, Corvetta D, Negri G, Mazzoleni G, Gast G, Steurer M, Gentilini I, Eisendle K & Fontanella F, 2016. Platelet gel: a new therapeutic tool with great potential. Blood transfuse review.p 1-7
Salman IA & Gundogdu C, 2010. Epithelial Healing In Experimental Corneal Alkali Wounds with Nondiluted Autologous Serum Eye Drops, Cutan. Ocul. Toxicol., vol.29, p. 116 – 121.
Semeraro F, Forbice E, Braga O, Bova A, Salvatore AD & Azzolini C, 2014. Evaluation of The Efficacy of 50 % Autologous Serum Eye Drops In Different Ocular Surface Pathologies. BioMed Research International, vol. 2014, artikel ID. 826970, p. 11.
Singh P, Tyagi M, Kumar Y, Gupta K & Sharma P, 2013. Ocular Chemical Injuries and Their Management. Oman J Ophthalmol, vol.6, no.2, p. 83 – 86.
Tanidir ST, Yuksel N, Altintas O, Yildiz DK, Sener E & Caglar Y, 2010. The Effect of Subconjunctival Platelet-Rich Plasma On Corneal Epithelial Wound Healing Cornea, vol.; 29, no.6, p. 664 – 669.
Copyright (c) 2023 Journal of Stem Cell Research and Tissue Engineering
This work is licensed under a Creative Commons Attribution 4.0 International License.
1. As an author you (or your employer or institution) may do the following:
- make copies (print or electronic) of the article for your own personal use, including for your own classroom teaching use;
- make copies and distribute such copies (including through e-mail) of the article to research colleagues, for the personal use by such colleagues (but not commercially or systematically, e.g. via an e-mail list or list server);
- present the article at a meeting or conference and to distribute copies of the article to the delegates attending such meeting;
- for your employer, if the article is a ‘work for hire', made within the scope of your employment, your employer may use all or part of the information in the article for other intra-company use (e.g. training);
- retain patent and trademark rights and rights to any process, procedure, or article of manufacture described in the article;
- include the article in full or in part in a thesis or dissertation (provided that this is not to be published commercially);
- use the article or any part thereof in a printed compilation of your works, such as collected writings or lecture notes (subsequent to publication of the article in the journal); and prepare other derivative works, to extend the article into book-length form, or to otherwise re-use portions or excerpts in other works, with full acknowledgement of its original publication in the journal;
- may reproduce or authorize others to reproduce the article, material extracted from the article, or derivative works for the author's personal use or for company use, provided that the source and the copyright notice are indicated, the copies are not used in any way that implies JSCRTE endorsement of a product or service of any employer, and the copies themselves are not offered for sale.
All copies, print or electronic, or other use of the paper or article must include the appropriate bibliographic citation for the article's publication in the journal.
2. Requests from third parties
Although authors are permitted to re-use all or portions of the article in other works, this does not include granting third-party requests for reprinting, republishing, or other types of re-use. Requests for all uses not included above, including the authorization of third parties to reproduce or otherwise use all or part of the article (including figures and tables), should be referred to JSCRTE by going to our website at http://e-journal.unair.ac.id/index.php/JSCRTE
3. Author Online Use
- Personal Servers. Authors and/or their employers shall have the right to post the accepted version of articles pre-print version of the article, or revised personal version of the final text of the article (to reflect changes made in the peer review and editing process) on their own personal servers or the servers of their institutions or employers without permission from JSCRTE, provided that the posted version includes a prominently displayed JSCRTE copyright notice and, when published, a full citation to the original publication, including a link to the article abstract in the journal homepage. Authors shall not post the final, published versions of their papers;
- Classroom or Internal Training Use. An author is expressly permitted to post any portion of the accepted version of his/her own articles on the author's personal web site or the servers of the author's institution or company in connection with the author's teaching, training, or work responsibilities, provided that the appropriate copyright, credit, and reuse notices appear prominently with the posted material. Examples of permitted uses are lecture materials, course packs, e-reserves, conference presentations, or in-house training courses;
- Electronic Preprints. Before submitting an article to an JSCRTE, authors frequently post their manuscripts to their own web site, their employer's site, or to another server that invites constructive comment from colleagues. Upon submission of an article to JSCRTE, an author is required to transfer copyright in the article to JSCRTE, and the author must update any previously posted version of the article with a prominently displayed JSCRTE copyright notice. Upon publication of an article by the JSCRTE, the author must replace any previously posted electronic versions of the article with either (1) the full citation to the work with a Digital Object Identifier (DOI) or link to the article abstract in JSCRTE homepage, or (2) the accepted version only (not the final, published version), including the JSCRTE copyright notice and full citation, with a link to the final, published article in journal homepage.
4. Articles in Press (AiP) service
JSCRTE may choose to publish an abstract or portions of the paper before we publish it in the journal. Please contact our Production department immediately if you do not want us to make any such prior publication for any reason, including disclosure of a patentable invention.
5. Author/Employer Rights
If you are employed and prepared the article on a subject within the scope of your employment, the copyright in the article belongs to your employer as a work-for-hire. In that case, JSCRTE assumes that when you sign this Form, you are authorized to do so by your employer and that your employer has consented to the transfer of copyright, to the representation and warranty of publication rights, and to all other terms and conditions of this Form. If such authorization and consent has not been given to you, an authorized representative of your employer should sign this Form as the Author.
6. JSCRTE Copyright Ownership
It is the formal policy of JSCRTE to own the copyrights to all copyrightable material in its technical publications and to the individual contributions contained therein, in order to protect the interests of the JSCRTE, its authors and their employers, and, at the same time, to facilitate the appropriate re-use of this material by others. JSCRTE distributes its technical publications throughout the world and does so by various means such as hard copy, microfiche, microfilm, and electronic media. It also abstracts and may translate its publications, and articles contained therein, for inclusion in various compendiums, collective works, databases and similar publications
Every accepted manuscript should be accompanied by "Copyright Transfer Agreement" prior to the article publication.
