FABRICATION OF PCL-COLLAGEN NANOFIBER USING CHLOROFORM-FORMIC ACID SOLUTION AND ITS APPLICATION AS WOUND DRESSING CANDIDATE
Downloads
In this study, polycaprolactone-collagen nanofiber was prepared with 10% w/v composition using a mixture of chloroform-formic acid. PCL was dissolved in chloroform while collagen was dissolved in formic acid. This research carried out optimization of electrospinning parameters such as flow rate, running time, and collector type to obtain optimum and suitable nanofiber to be applied as wound dressing. The most optimum nanofiber is made with flow rate 0.01 μL/h, running time is 3 hours, and using cylinder collector type. Characterization was performed for five different types of PCL-collagen nanofiber with different treatment, which nanofiber made with cylinder collector, plate collector, addition ofcitric acid, heating treatment, and nanofiber without the addition of collagen. PCL-collagen nanofiber produces smaller diameter about 200 - 600 nm. Based on the test of mechanical properties, addition of collagen causes its mechanical properties to be lower when compared to addition of crosslinking agents by heating or citric acid. The cytotoxicity test was carried out for PCL, PCL-collagen withaddition of citric acid, and PCL-collagen nanofiber treated by heating. PCL was chosen to compare the effect of collagen addition onnanofiber against cell viability. Collagen has an important role for growth, proliferation, and differentiation of cells in tissue engineering. PCL-collagen nanofiber which treated by heating provides better viability of 83.09% while compared to nanofiber with addition of citric acid, because citric acid acidic properties causing the environment around nanofiber have an extreme pH, it may affect the growth of cells and reduce its viability.
Keywords:Nanofiber, PCL, collagen, electrospinning, wound dressing, MTT Assay
Abrigo, M., McArthur, S.L., Kingshott, P., 2014, Electrospun Nanofibers as Dressings for Chronic Wound Care: Advances, Challenge, and Future Prospect, Macromolecular Bioscience, 14 (6), 772 – 792.
Amalia, N., 2012, Sintesis dan Karakterisasi Polimer Pati Sagu (Sago starch) dengan PEG 1000 menggunakan Asam Sitrat sebagai Crosslinking Agent, Skripsi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember.
Buttafoco. L., Kolkman, N.G., Engbers-Buijtenhuijs, P., Poot, A.A., Dijkstra, P.J., Vermes, I., Feijen, J., 2006, Electrospinning of Collagen and Elastin for Tissue Engineering Applications, Biomaterials, 27 (5), 724 – 734.
Callister Jr., W.D., & Rethwisch, D.G., 2014, Material Science and Engineering: An Introduction 9th Edition, John Wiley and Sons Inc, United State of America.
Dulnik, J., Denis, P., Sajkiewiwicz, P., Kolbuk, D., Choinska, E., 2016, Biodegradation of Biocomponent PCL/Gelatin and PCL/Collagen Nanofibers Electrospun from Alternative Solvent System, Polymer Degradation and Stability, 130, 10 – 21.
Franco, R.A., Min, Y.K., Yang, H.Y., Lee, B.T., 2012, On Stabilization of PVPA/PVA Electrospun Nanofiber Membrane and Its Effect on MaterialProperties and Biocompatibility, Journal of Nanomaterials, Hindawi Publishing Corporation.
Huang, Z.M., Zhang, Y.Z., Kotaki, M., Ramakrishna S.A., 2003, A Review on Polymer Nanofibers by Electrospinning and Their Applications in Nanocomposites, Composite Science and Technology, 63 (15), 2223 – 2253.
Khoswanto, C., Arijani, E., Soesilawati, P., 2008, Cytotoxicity Test of 40, 50 and 60% Citric Acid as Dentin Conditioner by using MTT Assay on Culture Cell Line, Dental Journal, 41 (3), 103 – 106.
Maneji, S.S., Scott, J., Page, J.Y.S., 2014, Process Optimization for the Electrospinning of Polycaprolactone Nanofibers Using Non-halogenated Solvents, International Conference on New Trends in Transport Phenomena, 82, 1 – 8.
Nomura, Y., Sakai, H., Ishii, Y., Shirai, K., 1996, Preparation and Some Properties of Type I Collagen from Fish Scales, Bioscience, Biotechnology, and Biochemistry, 60 (92), 2092 – 2094.
Pra, M.A.A.D., Valle, R.M.R.D., Maraschin, M., Veleirenho, B., 2017, Effect of Collector Design on The Morphological Properties of Polycaprolactone Electrospun Fibers, Materials Letters, (193), 154 – 157.
Rajput, M., 2012, Optimization of Electrospinning Parameters to Fabricate Aligned Nanofibers for Neural Tissue Engineering, Thesis, Department of Biotechnology and Medical Engineering National Institute of Technology Rourkela.
Rho, K.S., Jeong, L., Lee, G., Seo, B.M., Park, Y.J., Hong, S.D., Roh, S., Cho, J.J., Park, W.H., Min, B.M., 2006, Electrospinning of Collagen Nanofibers: Effects on The Behavior of Normal Human Keratinocytes and Early-stage Wound Healing, Biomaterials, 27 (8), 1452 – 1461.
Schueren, L.V.D., Schoenmaker, B.D., Kalaoglu, O.I., Clerck, K.D., 2011, An Alternative Solvent System for The Steady State Electrospinning of Polycaprolactone, European Polymer Journal, 47, 1256 – 1263.
Zeybek, B., Duman, M., Urkmez, A.S., 2014, Electrospinning of Nanofibrous Polycaprolactone (PCL) and Collagen-blended Polycaprolactone for Wound Dressing and Tissue Engineering, Usak University Journal of Material Science, 1, 121 – 134.
Zhang, Y.Z., Venugopal, J., Huang, Z.M., Lim, C.T., Ramakrishna, S., 2005, Characterization of The Surface Biocompatibility of The Electrospun PCL-Collagen Nanofibers Using Fibroblasts, Biomacromolecules
1. As an author you (or your employer or institution) may do the following:
- make copies (print or electronic) of the article for your own personal use, including for your own classroom teaching use;
- make copies and distribute such copies (including through e-mail) of the article to research colleagues, for the personal use by such colleagues (but not commercially or systematically, e.g. via an e-mail list or list server);
- present the article at a meeting or conference and to distribute copies of the article to the delegates attending such meeting;
- for your employer, if the article is a ‘work for hire', made within the scope of your employment, your employer may use all or part of the information in the article for other intra-company use (e.g. training);
- retain patent and trademark rights and rights to any process, procedure, or article of manufacture described in the article;
- include the article in full or in part in a thesis or dissertation (provided that this is not to be published commercially);
- use the article or any part thereof in a printed compilation of your works, such as collected writings or lecture notes (subsequent to publication of the article in the journal); and prepare other derivative works, to extend the article into book-length form, or to otherwise re-use portions or excerpts in other works, with full acknowledgement of its original publication in the journal;
- may reproduce or authorize others to reproduce the article, material extracted from the article, or derivative works for the author's personal use or for company use, provided that the source and the copyright notice are indicated, the copies are not used in any way that implies JSCRTE endorsement of a product or service of any employer, and the copies themselves are not offered for sale.
All copies, print or electronic, or other use of the paper or article must include the appropriate bibliographic citation for the article's publication in the journal.
2. Requests from third parties
Although authors are permitted to re-use all or portions of the article in other works, this does not include granting third-party requests for reprinting, republishing, or other types of re-use. Requests for all uses not included above, including the authorization of third parties to reproduce or otherwise use all or part of the article (including figures and tables), should be referred to JSCRTE by going to our website at http://e-journal.unair.ac.id/index.php/JSCRTE
3. Author Online Use
- Personal Servers. Authors and/or their employers shall have the right to post the accepted version of articles pre-print version of the article, or revised personal version of the final text of the article (to reflect changes made in the peer review and editing process) on their own personal servers or the servers of their institutions or employers without permission from JSCRTE, provided that the posted version includes a prominently displayed JSCRTE copyright notice and, when published, a full citation to the original publication, including a link to the article abstract in the journal homepage. Authors shall not post the final, published versions of their papers;
- Classroom or Internal Training Use. An author is expressly permitted to post any portion of the accepted version of his/her own articles on the author's personal web site or the servers of the author's institution or company in connection with the author's teaching, training, or work responsibilities, provided that the appropriate copyright, credit, and reuse notices appear prominently with the posted material. Examples of permitted uses are lecture materials, course packs, e-reserves, conference presentations, or in-house training courses;
- Electronic Preprints. Before submitting an article to an JSCRTE, authors frequently post their manuscripts to their own web site, their employer's site, or to another server that invites constructive comment from colleagues. Upon submission of an article to JSCRTE, an author is required to transfer copyright in the article to JSCRTE, and the author must update any previously posted version of the article with a prominently displayed JSCRTE copyright notice. Upon publication of an article by the JSCRTE, the author must replace any previously posted electronic versions of the article with either (1) the full citation to the work with a Digital Object Identifier (DOI) or link to the article abstract in JSCRTE homepage, or (2) the accepted version only (not the final, published version), including the JSCRTE copyright notice and full citation, with a link to the final, published article in journal homepage.
4. Articles in Press (AiP) service
JSCRTE may choose to publish an abstract or portions of the paper before we publish it in the journal. Please contact our Production department immediately if you do not want us to make any such prior publication for any reason, including disclosure of a patentable invention.
5. Author/Employer Rights
If you are employed and prepared the article on a subject within the scope of your employment, the copyright in the article belongs to your employer as a work-for-hire. In that case, JSCRTE assumes that when you sign this Form, you are authorized to do so by your employer and that your employer has consented to the transfer of copyright, to the representation and warranty of publication rights, and to all other terms and conditions of this Form. If such authorization and consent has not been given to you, an authorized representative of your employer should sign this Form as the Author.
6. JSCRTE Copyright Ownership
It is the formal policy of JSCRTE to own the copyrights to all copyrightable material in its technical publications and to the individual contributions contained therein, in order to protect the interests of the JSCRTE, its authors and their employers, and, at the same time, to facilitate the appropriate re-use of this material by others. JSCRTE distributes its technical publications throughout the world and does so by various means such as hard copy, microfiche, microfilm, and electronic media. It also abstracts and may translate its publications, and articles contained therein, for inclusion in various compendiums, collective works, databases and similar publications
Every accepted manuscript should be accompanied by "Copyright Transfer Agreement" prior to the article publication.
