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= http://dx.doi.org/10.20473/jscrte.v1i1.7567
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In this study, polycaprolactone-collagen nanofiber was prepared with 10% w/v composition using a mixture of chloroform-formic acid. PCL was dissolved in chloroform while collagen was dissolved in formic acid. This research carried out optimization of electrospinning parameters such as flow rate, running time, and collector type to obtain optimum and suitable nanofiber to be applied as wound dressing. The most optimum nanofiber is made with flow rate 0.01 μL/h, running time is 3 hours, and using cylinder collector type. Characterization was performed for five different types of PCL-collagen nanofiber with different treatment, which nanofiber made with cylinder collector, plate collector, addition ofcitric acid, heating treatment, and nanofiber without the addition of collagen. PCL-collagen nanofiber produces smaller diameter about 200 - 600 nm. Based on the test of mechanical properties, addition of collagen causes its mechanical properties to be lower when compared to addition of crosslinking agents by heating or citric acid. The cytotoxicity test was carried out for PCL, PCL-collagen withaddition of citric acid, and PCL-collagen nanofiber treated by heating. PCL was chosen to compare the effect of collagen addition onnanofiber against cell viability. Collagen has an important role for growth, proliferation, and differentiation of cells in tissue engineering. PCL-collagen nanofiber which treated by heating provides better viability of 83.09% while compared to nanofiber with addition of citric acid, because citric acid acidic properties causing the environment around nanofiber have an extreme pH, it may affect the growth of cells and reduce its viability.

Keywords:Nanofiber, PCL, collagen, electrospinning, wound dressing, MTT Assay


nanofiber, PCL, collagen, electrospinning, wound dressing, MTT Assay, stem cell, education, science

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Abrigo, M., McArthur, S.L., Kingshott, P., 2014, Electrospun Nanofibers as Dressings for Chronic Wound Care: Advances, Challenge, and Future Prospect, Macromolecular Bioscience, 14 (6), 772 – 792.

Amalia, N., 2012, Sintesis dan Karakterisasi Polimer Pati Sagu (Sago starch) dengan PEG 1000 menggunakan Asam Sitrat sebagai Crosslinking Agent, Skripsi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember.

Buttafoco. L., Kolkman, N.G., Engbers-Buijtenhuijs, P., Poot, A.A., Dijkstra, P.J., Vermes, I., Feijen, J., 2006, Electrospinning of Collagen and Elastin for Tissue Engineering Applications, Biomaterials, 27 (5), 724 – 734.

Callister Jr., W.D., & Rethwisch, D.G., 2014, Material Science and Engineering: An Introduction 9th Edition, John Wiley and Sons Inc, United State of America.

Dulnik, J., Denis, P., Sajkiewiwicz, P., Kolbuk, D., Choinska, E., 2016, Biodegradation of Biocomponent PCL/Gelatin and PCL/Collagen Nanofibers Electrospun from Alternative Solvent System, Polymer Degradation and Stability, 130, 10 – 21.

Franco, R.A., Min, Y.K., Yang, H.Y., Lee, B.T., 2012, On Stabilization of PVPA/PVA Electrospun Nanofiber Membrane and Its Effect on MaterialProperties and Biocompatibility, Journal of Nanomaterials, Hindawi Publishing Corporation.

Huang, Z.M., Zhang, Y.Z., Kotaki, M., Ramakrishna S.A., 2003, A Review on Polymer Nanofibers by Electrospinning and Their Applications in Nanocomposites, Composite Science and Technology, 63 (15), 2223 – 2253.

Khoswanto, C., Arijani, E., Soesilawati, P., 2008, Cytotoxicity Test of 40, 50 and 60% Citric Acid as Dentin Conditioner by using MTT Assay on Culture Cell Line, Dental Journal, 41 (3), 103 – 106.

Maneji, S.S., Scott, J., Page, J.Y.S., 2014, Process Optimization for the Electrospinning of Polycaprolactone Nanofibers Using Non-halogenated Solvents, International Conference on New Trends in Transport Phenomena, 82, 1 – 8.

Nomura, Y., Sakai, H., Ishii, Y., Shirai, K., 1996, Preparation and Some Properties of Type I Collagen from Fish Scales, Bioscience, Biotechnology, and Biochemistry, 60 (92), 2092 – 2094.

Pra, M.A.A.D., Valle, R.M.R.D., Maraschin, M., Veleirenho, B., 2017, Effect of Collector Design on The Morphological Properties of Polycaprolactone Electrospun Fibers, Materials Letters, (193), 154 – 157.

Rajput, M., 2012, Optimization of Electrospinning Parameters to Fabricate Aligned Nanofibers for Neural Tissue Engineering, Thesis, Department of Biotechnology and Medical Engineering National Institute of Technology Rourkela.

Rho, K.S., Jeong, L., Lee, G., Seo, B.M., Park, Y.J., Hong, S.D., Roh, S., Cho, J.J., Park, W.H., Min, B.M., 2006, Electrospinning of Collagen Nanofibers: Effects on The Behavior of Normal Human Keratinocytes and Early-stage Wound Healing, Biomaterials, 27 (8), 1452 – 1461.

Schueren, L.V.D., Schoenmaker, B.D., Kalaoglu, O.I., Clerck, K.D., 2011, An Alternative Solvent System for The Steady State Electrospinning of Polycaprolactone, European Polymer Journal, 47, 1256 – 1263.

Zeybek, B., Duman, M., Urkmez, A.S., 2014, Electrospinning of Nanofibrous Polycaprolactone (PCL) and Collagen-blended Polycaprolactone for Wound Dressing and Tissue Engineering, Usak University Journal of Material Science, 1, 121 – 134.

Zhang, Y.Z., Venugopal, J., Huang, Z.M., Lim, C.T., Ramakrishna, S., 2005, Characterization of The Surface Biocompatibility of The Electrospun PCL-Collagen Nanofibers Using Fibroblasts, Biomacromolecules


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