THE EFFECT OF INTRA-ARTICULAR APPLICATION OF ALLOGENIC MESENCHYMAL STEM CELL COMBINE WITH VEGF TO GRAFT TUNNEL HEALING AND AUTOGRAFT TENDON INTEGRATION IN ACL RECONSTRUCTION; A BIOMECHANICAL STUDY
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Graft-tunnel healing is the most determination factors in successful of Anterior Cruciate Ligament (ACL) reconstruction. The application of bone marrow derived mesenchymal stem cell (MSC) and vascular endothelial growth factor (VEGF) are one of integration biological augmentation method that often used in ACL reconstruction. Combination intra-articular post ACL reconstruction is expected to accelerate healing time and integration strength of tendon graft that used in bone tunnel. This method is experimental laboratory using animal model. The research is randomized post test only controlled group design. Five New Zealand white rabbit knee are used for ACL reconstruction with harmstring tendon graft and treated with combination allograft MSC and VEGF intra-articular, while five other rabbit knee as control without treatment. The evaluation is tensile test in third and six weeks post operation. Data was analyzed statistically and comparatively to compare the influence of MSC and VEGF to integration strength of graft tunnel healing. All the samples from treatment and control group found no complication after surgery. On third weeks evaluation, found a difference in failure tension load in both groups but not statistically significant (p>0,05), while on six weeks evaluation, found a statistically significant difference. Treatment group has a failure tension load higher than control group. While failure type of ACL tendon graft on 3 weeks evaluation, only 2 of 5 graft have pullout failure in treatment group. However, at three weeks in control group, the failure type of the tendon graft was a midsubtance rupture in intra-articular part during biomechanical tension test. The use of BM-MSC and VEGF intra-articular can increase tension failure load. It is expected that combination of BM-MSC and VEGF can increase integration process between bone graft and healing post ACL reconstruction, so that rehabilitation and mobilisation can be done earlier.
Keywords: Graft-tunnel healing, ACL reconstruction, Vascular Endothelial Growth Factor (VEGF), Bone Marrow Derived Mesenchymal Stem Cell (BMMSC)
Baxter FR, Bach JS, Detrez F, Cantournet S, Corte L, Cherkaoui M, et al. Augmentation of Bone Tunner Healing in Anterior Cruciate Ligament Grafts : Application of Calcium Phospates and Other Materials. Journal of Tissue Engineering. 2010; 2010: p. 12.
Bonasia DE, Amendola A. Graft choice in ACL reconstruction. In The Knee Joint : Surgical Techniques and Strategies.: Springer; 2012. p. 173-181.
Chen CH. Strategies to enhance tendon graft - bone healing in anterior cruciate ligament reconstruction. Chang Gung Med J. 2009; 32(5): p. 483-493.
Fuchs TF, Petersen W, Vordemvenne T, Stange R, Raschke M, Paletta JRJ. Influence of Synovial Fluid on Human Osteoblasts : an In Vitro Study. The Scientific World Journal. 2007;: p. 2012-2020.
Ho WP, Liau JJ, Cheng CK. Biomechanical Study of Bone-Patellar Tendon-Bone and Bone-ACL-Bone Grafts. Journal of Medical and Biological Engineering. 2002; 22(2): p. 103-107.
Lim JK, Hui J, Li L, Thambyah A, Goh J, Lee EH. Enhancement of Tendon Graft Osteointegration Using Mesenchymal Stem Cells in a Rabbit Model of Anterior Cruciate Ligament Reconstruction. Arthroscopy: The Journal of Arthroscopic and Related Surgery. 2004;: p. 899-910.
Pufe T, Harde V, Petersen W, Goldring M, TillMann B, Mentlein R. Vascular endothelial growth factor (VEGF) induces matrix metalloproteinase expression in immortalized chondrocytes. J Pathol. 2004; 202: p. 367-74.
Shrive N, Chimich D, Marchuk L, Wilson J, Brant R, Frank C. Soft-tissue "flaws" are associatedwith the material poperties of the healing rabbit medial collateral ligament. J Orthop Res. 1995; 13: p. 923-9.
Tohyama H, Yoshikawa T, Ju JY, Yasuda K. Revascularization in the tendon graft following anterior cruciate ligament reconstruction of the knee : Its mechanisms and regulation. Chang Gung Med J. 2009; 32(2): p. 133-139.
Yamakado K, Kitaoka K, Yamada H, Hashiba K, Nakamura R, Tomita K. The Influence of Mechanical Stress on Graft Healing in a Bone Tunnel. Arthroscopy : The Journal of Arthroscopic and Related Surgery. 2002; 18(1): p. 82-90.
Zelle BA, LatterMann C, Chhabra A, Fu FH, Huard J. Biological Considerations of Tendon Graft Incorporation Within the Bone Tunnel. In Operation Techniques in Orthopaedics.: Elsevier; 2005. p. 36-42.cc
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