INTRATUNNEL THE EFFECT OF ADMINISTRATION OF BONE MARROW MESENCHYMAL STEM CELLS (BM-MSCs) AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) TENDON-BONE TO INTERFACE HISTOLOGICAL GRAFT ANTERIOR CRUCIATE LIGAMENT APPEARANCE AFTER RECONSTRUCTION IN RABBITS
Downloads
The success of the Anterior Cruciate Ligament (ACL) reconstruction using a tendon graft is determined by integration in the bone tendon-graft interface on the bone tunnel. The use of stem cells and growth factors proved to accelerate the healing of the bone tendon-graft interface. The aim of this study was to inveestigate the difference of histology picture in the tendon-bone tunnel model after ACL reconstruction with intratunnel intravenous allogenic bone marrow mesenchymalstemcells (BM-MSCs) and intratunnel vascular endothelial growth factor (VEGF). This research used Post-Test Only Control Group design with 20 rabbits divided into treatment group and control group. Each group performed histologic image evaluation (thickness of collagen fiber or sharpey fiber) at week 3 and 6. Evaluation of histology overview at week 3 and week 6 showed a significantly thicker thickness of collagen fiber or sharpey fiber in treatment group compared with control group (p <0.05). Intravenous administration of BM-SCs and VEGF after ACL reconstruction can speed healing of the bone tunnel significantly from week 3 and 6. The study by Faridyan et al has concluded that intravenous BM-SCs + VEGF increased ultimate tension strength in the bone-tendon interface significantly. In this study, intravenous administration of BM-SCs and VEGF gave histologic images showing acceleration of bone tunnel healing.
Keywords:Anterior cruciate ligament reconstruction, allogenic bone marrow mesenchymal stem cells, vascular endothelial growth factor, graft tunnel healing, and Sharpey fiber.Adachi, N. et al., 2004. Reconstruction of The Aanterior cruciate ligament. Single- versus double-bundle Multistranded Hamstring Tendons. The Journal of bone and joint surgery. British volume,86, pp.515-520.
Alpert, JM et al., 2008. patellar tendon anterior cruciate Ligame Autograft for Reconstruction. In B. Cole, J. Sekiya, & LS Matthews, eds. Surgical Technique of the Shouder, Elbow, and Knee in Sports Medicine. Philadelphia: Saunders.
Anthony, AS & Brian, B., 2006. Sports Medicine. Orthopedic Surgery Essential 1st ed., Philadelphia: Lippincott Williams & Wilkins.
Ardern, CL et al., 2011. Return to sport following anterior cruciate ligament reconstruction surgery: a systematic review and meta-analysis of the state of play. British Journal of Sports Medicine, pp.1-11.
Arnoczky, SP et al., 1993. Anatomy of the anterior cruciate ligament. In Jackson DW, ed. The anterior cruciate cruciate ligament: Current and Future Concepts .New York: Raven Press, pp. 5-33.
Bartlett, RJ, Clatworthy, MG & Nguyen, TN V, 2001. Graft SELECTION IN RECONSTRUCTION OF THE anterior cruciate ligament. The Journal of bone and joint surgery. British volume,83 (July), pp.625-634.
Baxter, FR et al., 2010. Augmentation of Bone Tunnel Healing in anterior cruciate ligament grafts: Application of Calcium Phosphates and Other Materials. Journal of Tissue Engineering.
Boyer, J. &Meislin, RJ, 2010. Double-bundle versus single-bundle ACL Reconstruction. Bulletin of the NYU Hospital for Joint Diseases, 68 (2), pp. 119-126.
Deehan, DJ &Cawston, TE, 2005. The Biology of Integration of the anterior cruciate ligament. The Journal of bone and joint surgery. Britishvolume,87, pp.889-895.
Dong, Y. et al., 2012. Enhancement of Tendon - Bone Healing for anterior cruciate ligament (ACL) Reconstruction Using Bone Marrow-Derived Mesenchymal Stem Cells Infected with BMP-2. International Journal of Molecular Sciences,13, pp.13605-13620.
Faridyan, BV, 2015. At the Biomechanics Test Tendon Graft Model-Bone TunnelPost-Reconstruction of anterior cruciate ligament with allogenic Giving Bone Marrow Mesenchymal Stem Cells (BM-SCs) and Vascular Endothelial Growth Factor (VEGF) Intratunnel.Airlangga University
Ferdiansis, 2014. Effect of Intraarticular Mesenchimal Bone Marrow Stem Cells and Vascular Endothelial Growth Factor on Graft-Tunnel after ACL Reconstruction Integration .Airlangga University.
Fremerey, RW et al., 2000. Proprioception After Rehabilitation and Reconstruction in Knees with deficiency of the anterior cruciate ligament: A Prospective, Longitudinal Study. The Journal of bone and joint surgery.British volume,82 (6), pp.801-6. Available at: http://www.ncbi.nlm.nih.gov/pubmed/10990300.
Gardner, RL, 2002. Stem cells: Potency, Plasticity and Public Perception. Journal ofAnatomy,200 (3), pp.277-282.
Griffin, LY et al., 2006. Understanding and Preventing noncontact anterior cruciate ligament Injuries: A Review of the Hunt Valley II Meeting, January 2005. The American Journal of Sports Medicine, 34 (9), pp.1512-1532.
Griffin, MD, Ritter, T. & Mahon, BP, 2010. immunological aspects of allogeneic mesenchymal stem cell therapies. Human genetherapy,21 (12), pp.1641-55.
Hauser, RA et al., 2013. Ligament Injury and Healing: A Review of Current Clinical Diagnostics and Therapeutics. The Open Rehabilitation Journal,6, pp.1-20.
Hays, PL, 2008. The Role of macrophages in Early healing of a tendon graft in a bone tunnel. The Journal of Bone and Joint Surgery (American),90 (3), p.565.
Kaplan, LD & Fu, FH, 2004. Clinical Applications of Tissue Orthopedics Engieering: Tendons and ligaments. In VM Goldberg & AI Caplan, eds. Tissue Engineering Orthopedics Basic Science and Practice.New York:Taylor & Francis.
Kweon, C., Lederman, ES & Chhabra, A., 2013. Anatomy and Biomechanics of the cruciate ligaments and Their Implications Surgical. In Fanelli GC, ed. The Multiple Injured Knee Ligament: A Practical Guide to Management. New York: Springer-Verlag, pp. 17-27.
Lim, J.-K. et al., 2004. Enhancement of Tendon Graft Osteointegration Using Mesenchymal Stem Cells in A Rabbit Model of anterior cruciate ligament Reconstruction. Arthroscopy: The Journal of Arthroscopic & Related Surgery,20 (9), pp.899-910.
Liu, S. et al., 1997. Morphology and matrix composition during early tendon to bone healing. Clinical Orthopedic and Related Research, June (339), pp.253-60.
Lodish, H. et al., 2000. Collagen: the fibrous protein of the matrix. In Molecular Cell Biology.New York: WH Freeman Ed.
Ma, CB et al., 2007. Bone Morphogenetic Proteins-Signaling Plays A Role In Tendon-to-Bone Healing: A Study of rhBMP-2 and Noggin. The American journal of sports medicine, 35 (4), pp.597-604.
Martinek, V. et al., 2002. Enhancement of Tendon-Bone Integration of anterior cruciate ligament grafts With Bone Morphogenetic Protein-2 Gene Transfer: A histological and biomechanical Study. The Journal of bone andjoint surgery. American volume, 84, pp.1123-1131.
Milano, G., Deriu, L. & Fabbriciani, C., 2007. Graft-Tunnel Healing. In C. Prodromos et al., Eds. The anterior cruciate ligament: Reconstruction and BasicS cience .Philadelphia: Elsevier Health Sciences.
Myklebust, G. & Bahr, R., 2005. Return To Play Guideines After Surgery The anterior cruciate ligament. British Journal of Sports Medicine, 39, pp.127- 132.
Ouyang, HW, 2004. Use of Bone Marrow Stromal Cells for Tendon Graft-to-Bone Healing: histological and immuno histochemical Studies in a Rabbit Model. American Journal of Sports Medicine, 32 (2), pp.321-327. Available at: http://ajs.sagepub.com/content/32/2/321.long#ref-8.
Ratajczak, MZ et al., 2007. A Hypothesis For An Embryonic Origin of Pluripotent Oct-4 + Stem Cells In Adult Bone Marrow And Other Tissues. Leukemia, pp.860-867.
Rodeo, SA et al., 1993-Tendon Healing in a Bone Tunnel. The Journal of Bone and joint surgery,75 (12).
Sipola, A., 2009. Effects of Vascular Endothelial Growth Factor (VEGF-A) And Endostatin On Bone, Oulu: Oulu University Press.
Smith, BA, Livesay, GA & Woo, SL, 1993. Biology and Biomechanics of the anterior cruciate ligament. Clinics in Sports Medicine,12 (4), pp.637-70. Available at: http://www.ncbi.nlm.nih.gov/pubmed/8261518.
Starman, JS et al., 2007. Anatomy and Biomechanics of the anterior cruciate ligament. In C. Prodromos et al., Eds. The anterior cruciate Prodromos Ligament: Reconstruction and Basic Science. Philadelphia: Elsevier Health Sciences, pp. 3-11.
Sun, L. et al., 2012. Inhibitory Effect of synovial Fluid On-To-Bone Tendon Healing: An Experimental Study in Rabbits. Arthroscopy: The Journal of arthroscopic and related surgery: official publication of the Arthroscopy. Association of North America and the International Arthroscopy Association, 28 (9), pp.1297-305.
Weiler, A. et al., 2002 Tendon Healing In A Bone Tunnel. Part II. Arthroscopy:The Journal of Arthroscopic & amp; Related Surgery,18 (2), pp.124-135.
Williams III, RJ et al., 2004. The anterior cruciate ligament Reconstruction with a Four-Strand Hamstring Tendon Autograft. The Journal of Bone and Joint Surgery (American), 86-A, pp.225-232.
Woo, SL-Y. et al., 2006. Biomechanics of Knee ligaments: Injury, Healing, And Repair. Journal of Biomechanics,39 (1), pp.1-20.
Yoshikawa, T. et al., 2006. Effects of Local Administration of Vascular Endothelial Growth Factor on Mechanical Characteristics of the semitendinosus tendon graft anterior cruciate ligament After Reconstruction in Sheep. The American Journal of Sports Medicine, 34 (12), pp.1918-1925.
Zhang, F. et al., 2003. Effect of Vascular Endothelial Growth Factor On Rat Achilles Tendon Healing. Plastic and reconstructive surgery, 112, pp.1613- 1619.
1. As an author you (or your employer or institution) may do the following:
- make copies (print or electronic) of the article for your own personal use, including for your own classroom teaching use;
- make copies and distribute such copies (including through e-mail) of the article to research colleagues, for the personal use by such colleagues (but not commercially or systematically, e.g. via an e-mail list or list server);
- present the article at a meeting or conference and to distribute copies of the article to the delegates attending such meeting;
- for your employer, if the article is a ‘work for hire', made within the scope of your employment, your employer may use all or part of the information in the article for other intra-company use (e.g. training);
- retain patent and trademark rights and rights to any process, procedure, or article of manufacture described in the article;
- include the article in full or in part in a thesis or dissertation (provided that this is not to be published commercially);
- use the article or any part thereof in a printed compilation of your works, such as collected writings or lecture notes (subsequent to publication of the article in the journal); and prepare other derivative works, to extend the article into book-length form, or to otherwise re-use portions or excerpts in other works, with full acknowledgement of its original publication in the journal;
- may reproduce or authorize others to reproduce the article, material extracted from the article, or derivative works for the author's personal use or for company use, provided that the source and the copyright notice are indicated, the copies are not used in any way that implies JSCRTE endorsement of a product or service of any employer, and the copies themselves are not offered for sale.
All copies, print or electronic, or other use of the paper or article must include the appropriate bibliographic citation for the article's publication in the journal.
2. Requests from third parties
Although authors are permitted to re-use all or portions of the article in other works, this does not include granting third-party requests for reprinting, republishing, or other types of re-use. Requests for all uses not included above, including the authorization of third parties to reproduce or otherwise use all or part of the article (including figures and tables), should be referred to JSCRTE by going to our website at http://e-journal.unair.ac.id/index.php/JSCRTE
3. Author Online Use
- Personal Servers. Authors and/or their employers shall have the right to post the accepted version of articles pre-print version of the article, or revised personal version of the final text of the article (to reflect changes made in the peer review and editing process) on their own personal servers or the servers of their institutions or employers without permission from JSCRTE, provided that the posted version includes a prominently displayed JSCRTE copyright notice and, when published, a full citation to the original publication, including a link to the article abstract in the journal homepage. Authors shall not post the final, published versions of their papers;
- Classroom or Internal Training Use. An author is expressly permitted to post any portion of the accepted version of his/her own articles on the author's personal web site or the servers of the author's institution or company in connection with the author's teaching, training, or work responsibilities, provided that the appropriate copyright, credit, and reuse notices appear prominently with the posted material. Examples of permitted uses are lecture materials, course packs, e-reserves, conference presentations, or in-house training courses;
- Electronic Preprints. Before submitting an article to an JSCRTE, authors frequently post their manuscripts to their own web site, their employer's site, or to another server that invites constructive comment from colleagues. Upon submission of an article to JSCRTE, an author is required to transfer copyright in the article to JSCRTE, and the author must update any previously posted version of the article with a prominently displayed JSCRTE copyright notice. Upon publication of an article by the JSCRTE, the author must replace any previously posted electronic versions of the article with either (1) the full citation to the work with a Digital Object Identifier (DOI) or link to the article abstract in JSCRTE homepage, or (2) the accepted version only (not the final, published version), including the JSCRTE copyright notice and full citation, with a link to the final, published article in journal homepage.
4. Articles in Press (AiP) service
JSCRTE may choose to publish an abstract or portions of the paper before we publish it in the journal. Please contact our Production department immediately if you do not want us to make any such prior publication for any reason, including disclosure of a patentable invention.
5. Author/Employer Rights
If you are employed and prepared the article on a subject within the scope of your employment, the copyright in the article belongs to your employer as a work-for-hire. In that case, JSCRTE assumes that when you sign this Form, you are authorized to do so by your employer and that your employer has consented to the transfer of copyright, to the representation and warranty of publication rights, and to all other terms and conditions of this Form. If such authorization and consent has not been given to you, an authorized representative of your employer should sign this Form as the Author.
6. JSCRTE Copyright Ownership
It is the formal policy of JSCRTE to own the copyrights to all copyrightable material in its technical publications and to the individual contributions contained therein, in order to protect the interests of the JSCRTE, its authors and their employers, and, at the same time, to facilitate the appropriate re-use of this material by others. JSCRTE distributes its technical publications throughout the world and does so by various means such as hard copy, microfiche, microfilm, and electronic media. It also abstracts and may translate its publications, and articles contained therein, for inclusion in various compendiums, collective works, databases and similar publications
Every accepted manuscript should be accompanied by "Copyright Transfer Agreement" prior to the article publication.
