COLLAGEN ANALYSIS OF GRAFT IN BONE TUNNEL MODEL ANTERIOR CRUCIATE LIGAMENT (ACL) RECONSTRUCTION WITH INTRATUNNEL ALLOGENIC BONE MARROW MESENCHYMAL STEM CELLS (MSCs) AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF)
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ACL reconstruction measures aim to obtain stable knees, and reduce the risk of further injury to the meniscus and joint surfaces. Acceleration of the integration process between the tendon graft and the bone tunnel will improve the final result of ACL reconstruction. The purpose of this study was to investigate the collagen composition of the bone tunnel graft model of anterior cruciate ligament reconstruction with intravenous allogenic bone marrow mesenchymal stem cells and vascular endothelial growth factor in experimental animals. The design of this study was Post-test only Control Group Design using 20 rabbits divided into treatment group and control group. Collagen immunohistochemical evaluation was performed at weeks 3 and 6. Evaluation at week 3 obtained the area of collagen type-1 in the higher treatment group at treatment (p <0.001). In the 6th week evaluation, it was found that the area of collagen type-1 in the treatment group was higher (p <0.05). Type-1 collagen at week 6 did not differ significantly with week 3 (p> 0.05). Provision of allogenic bone marrow mesenchymal stem cells and intratonal vascular endothelial growth factor in ACL reconstruction enhanced the formation of collagen type-1 which is the acceleration of incorporation of the graft tendon process with bone tunnel.
Keywords : Anterior Cruciate Ligament, allogenic bone marrow mesenchymal stemcells, vascular endothelial growth factor, graft and collagen.Ardern C, Taylor N, Feller J, Webster K. 2012. Return-to-sport Outcomes at 2 to 7 years anterior cruciate Lihgament After Reconstruction Surgery. American Journal of Sports Medicine. January; 40 (1): p. 41-48.
Changlong Y, Lin L, Wei X. 2011. The Different Effects of TGF-β1, VEGF and PDGF on the remodeling of anterior cruciate ligament graft, Targets in Gene Therapy. In You Y. Targets in Gene Therapy. Shanghai: Intechopen.
Deehan DJ, Cawston TE. 2005. The Biology of Integration of anterior cruciate ligament. British Editorial Society of Bone and Joint Surgery; 87 (B): p. 889-895.
Dordevic M, Hirschmann MT. 2014. Mechanism of Injury ACL Tear. In Siebold R. anterior cruciate ligament Reconstruciton A Practical Gudie. New York: Springer Heidelberg; p. 49-52.
Kjaer M, Krogsgaard M, Magnusson P, Engebretsen L, Roos H, Takala T, et al., Editors. Textbook of Sports Medicine. Oxford: Blackwell; p. 561.
Kanazawa T, Soejima T, Tabuchi K. 2012. histological analysis on the tendon-to- bone healing utilizing bone marrow- derived MSCs in ACL reconstruction without amodels. bone tunnel ORS 2012 Annual Meetin; 1341.
Kweon C, Lederman ES, Chhabra A. 2013. Anatomy and Biomechanics of the cruciate ligaments and Their Implications Surgical. In G. Fanelli he MultipleLigament: Injured Knee A Practical Guide to Management. New York: Springer Science; p. 17-26.
Lui PPY, Zhang P, Chan KM, Qin L. 2010. Biology and Augmentation of Tendon- Bone Repair Insertion. Journal of Orthopedic Surgery and Research. August; 5 (59): p. 1-14.
Myklebust G, Bahr R. 2005. Return to Play Guidelines AfterThe anterior cruciate Surgery ligament. British Journal of Sports Medicine. April; 39 (3): p. 127- 131.
Ouyang HW, Goh J, Lee EH. 2004. Use of Bone Marrow Stromal Cells for Tendon Graft-to-Bone Healing histological and immuno histochemical Studies in a Rabbit Model. The American Journal of Sports Medicine; 32 (2): p. 321-326.
Pastides PS, Khan W. 2011. Tendon and Ligament Injuries: The Evolving Role of Stem Cells and Tissue Engineering. British Journal of Medicine and Medical Research; 1 (4): p. 569-580.
Petersen W, Tilllmann B. 1999. Structure and Vascularization of cruciate ligaments of the Human Knee Joint. Anatomy Embryology; 200: p. 325-344.
Tomita M, Yasuda K, Mikami S, Sakai T, Yamazaki S, Tohyama H. 2001. Comparisons of intraosseous Graft Healing Doubled Between the flexor tendon graft and the Bone-patellar tendon-bone graft in anterior cruciate ligament Reconstruction. Arthroscopy: The Journal of Arthroscopic and Related Surgery; 17 (5).
Yu C, Lin L, Wei X. 2011. The Different Effects of TGF-β1, VEGF and PDGF on the remodeling of anterior cruciate ligament graft. In Yongping Y, editor. Gene Therapy. Shanghai: Intech; p. 390-396.
Zelle B, Lattermann C, Chhabra A, Fu F, Huard J. 2005. Biological Considerations of Tendon Graft Incorporation Within the Bone Tunnel. Operative Techniques in Orthopedics; 15: p. 36-42.
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