S. Ahmad Taufik

Abstract views = 408 times | downloads = 242 times


The joint cartilage defectfullthickness is still a problem today because its currenttreatment still has not delivered maximum results. Current treatment uses cartilage enginering using mesenchymal stem cells alone and or combining growth factor. The aim of this study was to investigate the effect of intra-articular injection of Allogenic bone marrow mesenchymal stem cell - Platelet rich plasma (BMSCs-PRP) on regeneration of cartilage defect fullthickness in rabbits. The design of this study was a post-test only control group design using 36 New Zealand white rabbits divided into three groups. Each group were treated with PRP, BMSCs and BMSCs-PRP. Results were evaluated after 10 weeks. In the evaluation, macroscopic images showed the best healing in the BMSCs-PRP group. Histopathologic examination showed that in the MSCs-PRP group, there was a significant increase in the number of chondrocytes (p = 0,000), cartilage area (p = 0,000), as well as the number of Agecoprogenitorexpec- tion cells (p = 0,000) and collagen type 2 (p = 0,000). BMSCs were able to differentiate into condroblasts which then synthesize aggressive and collagen type 2. Platelet rich plasma (PRP) contains growth factor BMP, TGF, FGF and IGF which can accelerate the occurrence of MSCs differentiation. Intra-articular injections Allogenic bone marrow mesenchymal stem cell (BMSCs-PRP) is able to regenerate and cure full-thickness joint cartilage defects through differentiation of MSCs into condroblasts.

Keyword: Allogenic, Bone marrow Mesenchymal stem cell, Cartilage, Platelet rich plasma, Full-thickness.


Allogenic, Bone marrow Mesenchymal stem cell, Cartilage, Platelet rich plasma, Full-thickness.

Full Text:



Aroen A, Loken S, Heir S, Alvik E, Ekeland A, Granlund O G, Engebretsen L. Articular Cartilage Lesions in 993 Consecutive Knee Arthroscopies.American Journal of Sports Medicine, 2004 (32): 211-215.

Akeson W, Amiel D, Gershuni D. Articular cartilage physiology and metabolism. In: Resnick D, ed. Diagnosis of bone and joint disorders. Philadelphia: WB Saunders, 1994:23-1-23-31.

Athanasiou KA, Shah AR and Hernandez RJ. Basic Science of Articular Cartilage Repair. J. Clin Sport Me, 2002; 39(4):223 – 47.

Benninghoff A. Form und bau der Geleknorpel in ihren Bezeihungen zur Funktion. Z Zellforsch Mikrosk Anat 2, 1925: 783-825.

Buckwalter JA, Mankin HJ and Grodzinsky AJ. Articular cartilage and osteoarthritis. Instr Course Lect, 2005; 54: 465–80.

Bunnell BA, Kluge KA, Lee-Lin SQ, et al. Transplantation of transduced nonhuman primate CD34 cells using a gibbon ape leukemia virus vector: restricted expression of the gibbon ape leukemia virus receptor to a subset of CD34 cells. Gene Ther, 1999; 6: 48-56

Caplan AI, Dennis JE. Mesenchymal stem cells as trophic mediators. J Cellular Biochem, 2006; 98: 1076-1084.

Caplan AI. Review: mesenchymal stem cells: cell-based reconstructive therapy in orthopedics. Tissue Eng, 2005; 11(7-8): 1198-211.

Chen FH, Song Li, Mauck RI and Li WJ. Mesenchymal Stem Cells. In:Lanza R, Langer R, Vacanti J eds. Principles of Tissue Engineering. 3rd Edition.

Chen H, Sun J, Hoemann CD, et al. Drilling and microfracture lead to different bone structure and necrosis during bone-marrow stimulation for cartilage repair. J Orthop Res, 2009; 27(11): 1432- 8.

Clutterbuck AL, Asplin KE, Harris P, Allaway D, Mobasheri A. Targeting matrix metalloproteinases in inflammatory conditions. Curr Drug Targets, 2009; 10(12): 1245-54.

Cook SD, Barrack RL and Skinner HB. Basic Science in Orthopaedic Surgery, In Skinner HB, ed. Current Diagnosis and Treatment in Orthopaedics. 2nded. New York : Lange Medical Book, 2000: 8 – 11.

Darmadi SH. Mechanism of Cartilage Healing.Surabaya : Lab/SMF Orthopaedi dan Traumatologi FK Unair / RSUD Dr. Soetomo, 2002.

Gregory JS, Waarsing JH, Day J, Pols HA, Reijman M., Weinans H and Aspden RM. Early identification of radiographic osteoarthritis of the hip using an active shape model to quantify changes in bone morphometric features: can hip shape tell us anything about the progression of osteoarthritis? Arthritis Rheum, 2007; 56: 3634–3643.

Gobbi A and Bathan L. Biological Approach for Cartilage Repair.J Knee Surg, 2009; 2: 36–44.

Gupta PK, Das AK, Chulikana A and Majumdar AS.(2012) Mesenchymal stem cells for cartilage repair in osteoarthritis. Stem cell & Therapy. 3: 25.

Henderson JH, Welter JF, Mansour JM, Niyibizi C, Caplan, A. I., and Dennis, J. E. Cartilage tissue engineering for laryngotracheal reconstruction: comparison of chondrocytes from three anatomic locations in the rabbit. Tissue Eng, 2007; 13(4): 843-53

Iliz MZ, Mok MT, Williamson OD, Campbell MA, Hughes CE, Handley CJ. Catabolism of aggrecan by explant cultures of human articular cartilage in the presence of retinoic acid. Arch Biochem Biophys, 1995: 322(1); 22-30.

Im GI, Kim DY, Shin JH, Hyun CW, Cho WH. Repair of cartilage defect in the rabbit with cultured mesenchymal stem cells from bone marrow. J Bone Joint Surg Br, 2001; 83: 289-294.

Khan WS, Adesida AB, Hardingham TE. Hypoxic conditions increase hypoxia-inducible transcription factor 2alpha and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients. Arthtitis research & therapy, 2005; 9: R55.

Koga H, Engebretsen L, Brinchmann JE, Muneta T and Sekiya K. Mesenchymal stem cell-based therapy for cartilage repair: a review. 2009; 17: 1289 – 97.

Lewthwaite J, Blake S, Thompson RC, et al. Antifibrotic action of interleukin-1 receptor antagonist in lapine monoarticular arthritis. Ann Rheum Dis, 1995; 54: 591-596.

Magnussen RA, Dunn WR, Carey JL, Spindler KP. Treatment of focal articular cartilage defects in the knee: a systematic review. Clin Orthop Relat Res, 2008; 466: 952-962.

Mankin HJ, Buckwaller JA, Grodzinsky AJ. Articular cartilage and osteoarthritis. Inst Course Lect, 2005; 54: 465-480.

Muschler GF, Nakamoto C, Griffith LG. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg. Am., 2004: 86-A; 1541-1558.

McIlwraith CW, Frisbie DD, Rodkey WG, Kisiday JD, Werpy NM, Kawcak CE, Steadman JR. Evaluation of intra-articular mesenchymal stem cells to augment healing of microfractured chondral defects. Arthroscopy, 2011 Nov; 27(11): 1552-61.

Nejadnik H, Hui JH, Feng Choong EP, et al. Autologous bone marrow-derived mesenchymal stem cells versus autologous chondrocyte implantation: an observational cohort study. Am J Sports Med, 2010; 38: 1110-1116.

Punwar S, Khan WS. Mesenchymal Stem Cells and Articular Cartilage Repair: Clinical Studies and Future Direction. Open Orthop J, 2011; 5: 296-301

Redman SN, Oldfield SF and Archer CW. (2005) Current strategies for articular cartilage repair. Eur Cell Mater, 2005 Apr 14; 9: 23 – 32.

Rofi’i dan Dwikora NU.(2011) Optimal Consentrasion Platelet rich plasma and growth factor with varius sentrifugation technique.Tesis, UniversitasAirlangga.

Sato M, Uchida K, Nakajima H, Miyazaki T, Guerrero AR, Watanabe S, Roberts S, Baba H. Direct transplantation of mesenchymal stem cells into the knee joints of hartley strain guinea pigs with spontaneous osteoarthritis. Arthritis Res Ther, 2012; 14:R31.

Sharma B, Williams CG, et al. Designing zonal organization into tissue-engineered cartilage. Tissue Eng, 2007; 13(2): 405-414

Sun Y, Lu Y, Chen S, Prasad M, Wang X, Zhu Q, Zhang J, et al. Key proteolytic cleavage site and full-length form of DSPP. J Dent Res, 2010; 89: 498-503.

Wakitani S, Goto T, Pineda SJ, Young RG, Mansour JM, Caplan AI, Goldberg VM. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J Bone Joint Surg Am, 1994; 76: 579-592.

Whitman DH, Berry RL, Green DM. Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg, 1997; 55(11): 1294-1299.


  • There are currently no refbacks.

Copyright (c) 2018 Journal of Stem Cell Research and Tissue Engineering

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

View Stats

Creative Commons License
JSCRTE by Unair is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.