BIOMECHANIC STUDY OF GRAFT BONE TUNNEL MODEL IN ANTERIOR CRUCIATE LIGAMENT RECONSTRUCTION USING INTRATUNNEL ALLOGENIC BONE MARROW MESENCHYMAL STEM CELLS (BM-MSCs) AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF)

brian vicky faridyan

= http://dx.doi.org/10.20473/jscrte.v2i1.9262
Abstract views = 111 times | views = 97 times

Abstract


Successful anterior cruciate ligament (ACL) reconstruction using tendon graft requires good and rapid integration between the tendon graft and the bone tunnel. The strength of the tendon-bone tunnel graft in the initial phase is very important to facilitate aggressive rehabilitation and as early as possible to support rapid recovery to normal activities. The objective of this study was to determine ultimate tension strength (UTS) on the femoral tendon-bone tunnel graft model after reconstruction of anterior cruciate ligament (ACL) by administering allogenic bone marrow mesenchymal stemcells (BM-MSCs) and vascular endothelial growth factor (VEGF) intratunnel in experimental animals. The design of this research was Post-Test Only Control Group Design using 24 rabbits divided into treatment and control group. Biomechanical evaluation was done at week 3 and 6. Evaluation at week 3 found ultimate tension strength of treatment group significantly higher than control (p <0,05). In the 6th week evaluation, Ultimate tension strength was found that the treatment group significantly higher than the control group (p <0.05). Ultimate tension strength at week 3 did not differ significantly with week 6 (p> 0.05). Intravenous administration of BM-MSCs and VEGF on ACL reconstruction increased ultimate tension strength in graft-bone tunnel significantly since week 3. The study of Ferdiansis et al using BM-MSCs and VEGF intraarticular, only showed a significant increase in ultimate tension strength in graft-bone tunnel since week 6. Comparison of this method indicates acceleration in incorporation of tendon graft with bone tunnel on intratunnel method better thaninvitro intraarticular method.

Keywords : Anterior cruciate ligament, allogenic bone marrow mesenchymal stem cells, vascular endothelial growth factor and biomechanic study.


Keywords


Anterior cruciate ligament, allogenic bone marrow mesenchymal stem cells, vascular endothelial growth factor and biomechanic study.

Full Text:

PDF

References


Alpert, J.M., Brush-Joseph, C.A., Bach, J. & Bernard, R., 2008. Patellar Tendon Autograft for Anterior Cruciate Ligament Reconstructiom. In Surgical Techniques of The Shoulder, Elbow, and The Knee in Sports Medicine.Philadelphia, PA, USA: Elseviers Saunders.

Amiel, D., Frank, C. & Harwood, F., 1984. Tendons and ligaments: a morphological and biochemical comparison. J Orthop Res, 1, pp.257– 65.

Anderson, A.F., Dome, D.C. & Gautam, S., 2001. Correlation of anthropometric measurements, strength, anterior cruciate ligament size, and intercondylar notch characteristics to sex differences in anterior cruciate ligament tears. Am J Sports Med, (29), pp.58–63.

Bach, J.M., Hull, M.L. & Patterson, H.A., 1997. Direct measurement of strain in the posterolateral bundle of the anterior cruciate ligament. J.Biomech, 30(3), pp.281-83.

Baxter, F.R. & Bach, J.S., 2010. Augmentation of Bone Tunnel Healing in Anterior Cruciate Ligament Grafts: Application of Calcium Phosphates and Other Materials. Journal of Tissue Engineering.

Chandler, J.W. & Creighton, R.A., 2008. Patellar Tendon Allograft for Anterior Cruciate Ligament Reconstruction. In Surgical Techniques of TheShoulders, Elbow, and Knee in Sport Medicine. Philadelphia, PA, USA: Elseviers Saunders.

Colombet, P., Robinson, J. & Christel, P.l., 2006. Morphology of anterior cruciate ligament attachments for anatomic reconstruction: a cadaveric dissection and radiographic study. Arthroscopy, 22, pp.984–92.

Da Silva, E.M. & Albano, M.B., 2013. Knee Ligament Injuries: Biomechanics Comparative Study of Two Suture Technique in Tendon Analysis. Rev Bras Ortop, 48(1), pp.80-86.

Deehan, D.J. & Cawston, T.E., 2005. The Biology of Integration of Anterior Cruciate Ligament. British Editorial Society of Bone and Joint Surgery, 87(8), pp.889-95.

Dong, Y. et al., 2012. Enhancement of Tendon-Bone Healing for Anterior Cruciate Ligament (ACL) Reconstruction Using Bone Marrow- Derived Mesenchymal Stem Cells Infected with BMP-2. International Journal ofMolecular Sciences, 13, pp.13605-20.

Duthon, V.B., Barea, C. & Abrassart, S., 2006. Anatomy of the anterior cruciate ligament. Knee Surg Sports Traumatol Arthrosc, 14, pp.204–13.

Feng, L., Jia, H. & Yu, C., 2007. ACL reconstruction in a rabbit model using irradiated Achilles allograft seeded with mesenchymal stem cells or PDGF-B gene- transfected mesenchymal stem cells. Knee Surgery Sport Traumatololgy Arthroscopy, Knee Surgery Sport Traumatololgy Arthroscopy, p.1219027.

Ferdiansis, e.a., 2014. Effect of intraarticular Bone Marrow Mesenchimal Stem Cells and Vascular Endothelial Growth Factor on Graft-Tunnel Integration after ACL Reconstruction., 2014. National Indonesian Orthopaedic Congress.

Freeman, A., 2001. How the Knees Move. Current Orthopaedics, 15, pp.444 - 450.

Fukubayashi, T., Torzilli, P.A., Sherman, M.F. & Warren, R.F., 1982. An in vitro biomechanical evaluation of anterior- posterior motion of the knee. Tibial displacement, rotation, and torque. J. Bone Joint Surg Am., 64(258-264), pp.258-64.

Gotter, D. & Schmidt-Wiethoff, 2007. Biomechanics of the Anterior Cruciate Ligament and Implications for Surgical Reconstruction. In Strategies Trauma Limb Reconstr. pp.1-12.

Griffin, M.D., Ritter, T. & Mahon, B.P., 2010. Immunological Aspects of Allogeneic Mesenchymal Stem Cells Therapies. Human gene therapy, 21, pp.1641-55.

Harner, C.D., Baek, G.H. & Vogrin, T.M., 1999. Quantitative analysis of anterior cruciate ligament insertions. Arthroscopy, 15, pp.741–49.

Hays, P. et al., 2008. The Role of Macrophages in Early Healing of a Tendon Graft in a Bone Tunnel. Journal Bone Joint Surgery America, 90-A(3), pp.565-75.

HW, O., JC, G. & EH, L., 2004. Use of bone marrow stromal cells for tendon graft- to-bone healing: histological and immunohistochemical studies in a rabbit model. Am J Sports Med, 32, pp.321-27.

Jones, C.D. & Grimshaw, P.N., 2011. The Biomechanics of the Anterior Cruciate Ligament and Its Reconstruction. In Theoretical Biomechanics. pp.361– 381.

Ju, Y.J. et al., 2006. Effects of Local Administration of Vascular Endothelial Growth Factor on Properties of the in Situ Frozen- Thawed Anterior Cruciate Ligament in Rabbits. The American Journal of Sports Medicine, 34(1), pp.84-89.

Kanaya, A. et al., 2007. Intra-articular Injection of Mesenchymal Stromal Cells in Partially Torn Anterior Cruciate Ligaments in a Rat Model. Arthroscopy:The Journal of Arthroscopic and Related Surgery, 23(6), pp.610-17.

Kanazawa, T., Soejima, T. & Tabuchi, K., 2012. Histological analysis on the tendon-to-bone healing utilizing bone marrow-derived MSCs in ACL reconstruction model without a bone tunnel. In ORS 2012 Annual Meeting., 2012.

Kaplan, L.D. & Fu, F., 2004. Clinical Applications of Orthopedic Tissue Engineering: Liganlents and Tendons. In A.C. VM Goldberg, ed. OrthopedicTissue Engineering Basic Science and Practice. New York, USA: Marcel DekkerInc.

Kohno, T. et al., 2007. Immunohistochemical demonstration of growth factors at the tendon- bone interface in anterior cruciate ligament reconstruction using a rabbit model. J. Orthop. Sci., 12, pp.67-73.

Krosshaug, T. & Slauterbeck, J.R., 2006. Biomechanical Analysis od Anterior Cruciate Ligament Injury Menchanisms: Three-Dimensional Motion Reconstruction from Video Sequences. Scandinavian Journal of Medicine and Science in Sports.

Lane, J.G., Kaufman, K., Rangger, C. & Daniel, D.M., 1994. The anterior cruciate ligament in controlling axial rotation. An evaluation of its effect. Am. J.Sports Med., 22(2), pp.289-93.

L'Insalata, J.C., Klatt, B. & Fu, F.H., 1997. Tunnel expansion following anterior cruciate ligament reconstruction: A comparison of hamstring and patellar tendon autograft. Knee Surg Sports Traumatol Arthrosc, 5, pp.234-38.

Lipke, J.M., Janecki, C.J. & Nelson, C.L., 1981. The role of incompetence of the anterior cruciate and lateral ligaments in anterolateral dan anteromedial instability. A biomechanical study of cadaver knees. J Bone Joint Surg Am, 63(954-960).

Ma, C.B. et al., 2007. Bone morphogenetic proteins-signaling plays a role in tendon-to-bone healing: A study of rhBMP-2 and noggin. Am. J. Sports Med., 35, pp.597–604.

Mall, N.A., Van Thiel, G.S., Bedi, A. & Cole, B.J., 2013. Graft Selectionin Anterior Cruciate Ligament Reconstruction. [Online] Available at: http://www.rockfordortho.com/wp- content/uploads/2012/07/Graft- Selection-in- ACL- Surgery.pdf [Accessed 11 January 2015]

McCarty, L.P., 2005. Anatomy, Biology, and Biomechanics of Pattelar Tendon Autograft Anterior Cruciate Ligament Reconstruction. Techniques inOrtop.

Milano, G., Deriu, L. & Fabbriciani, C., 2008. Graft-Tunnel Healing. In C. Brown et al., eds. The Anterior Cruciate Ligament: Reconstruction and Basic Science. Philadelphia, PA, United State of America: SAUNDERS Elsevier.

Odensten, M. & Gillquist, J., 1985. Functional anatomy of the anterior cruciate ligament and a rationale for reconstruction. J Bone Joint Surg Am, 67, pp.257–62.

Oe, K., Kushida, T. & Okamoto, N., 2011. New strategies for anterior cruciate ligament partial rupture using bone marrow transplantation in rats. StemCells Development, 20, pp.671- 79.

Petersen, W. & Tillmann, B., 2002. Anatomy and function of the anterior cruciate ligament. Orthopäde, 31, pp.710–18.

Pinczewski, L., Clingeleffer, A., Otto, D. & Bonar, F., 1997. Integration of Hamstring Tendon Graft With Bone in Reconstruction of the Anterior Cruciate Ligament. Arthroscopy:The Journal of Arthroscopic and Related Surgery, 13(5), pp.641-43.

Reinert, R.B. et al., 2013. Vascular endothelial growth factor-a and islet vascularization are necessary in developing, but not adult, pancreatic islets. PubMed.gov Diabetes., 62(12), pp.4154-64.

Rodeo, S.A. et al., 1993. Tendon-healing in a bone tunnel. A biomechanical and histological study in the dog. Journal Bone Joint SurgeryAmerica, 72(12), pp.1795-803.

Sakane, M., Fox, R.J. & Woo, S.L., 1997. In situ forces in the anterior cruciate ligament and its bundles in response to anterior tibial loads. J Orthop Res, 15, pp.285–93.

Song, E.K. et al., 2004. Failure of Osteointegration of Hamstring Tendon Autograft After Anterior Cruciate Ligament Reconstruction. Arthroscopy: The Journal of Arthroscopic and Related Surgery, 20(4), pp.424-28.

Tomita, F. et al., 2001. Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction. Arthroscopy, 17(5).

Weiler A et al., 2002. Tendon Healing in a Bone Tunnel. Part I: Biomechanical Results After Biodegradable Interference Fit Fixation in a Model of Anterior Cruciate Ligament Reconstruction in Sheep. Arthroscopy: The Journalof Arthroscopic and Related Surgery, 18(2), pp.113-23.

Weiler, A. et al., 2002. Tendon Healing in a Bone Tunnel Part II: Histologic Analysis After Biodegradable Interference Fit Fixation in a Model of Anterior Cruciate Ligament Reconstruction in Sheep. Arthroscopy: The Journal of Arthroscopic and Related Surgery, 72(12), pp.113-23.

Zelle, B.A. et al., 2005. Biological Consideration of Tendon Graft Incorporation Within the Bone Tunnel. Elsevier, Operative Technique in Orthopaedics.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Journal of Stem Cell Research and Tissue Engineering

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.