EVALUASI JARINGAN DALAM STUDI IN VIVO PEMBERIAN EKSTRAK PROPOLIS PADA MODEL LUKA INFEKSI

Winona May Hendrata, Willy Sandhika, Agung Dwi Wahyu Widodo

= http://dx.doi.org/10.20473/mbiom.v29i2.2019.32-39
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Abstract


Background: Infection in wound could lead to various consequences including scarring and occurrence of sepsis. With increasing prevalence of resistant strain bacteria, option for treating infected wound need to be constantly expanded. Propolis, a product of honey bee, is a potential treatment which has been studied to have various effects including antibacterial, anti-inflammatory, and antioxidant. Objective: The study aims to assess the effect of propolis toward wound healing, by evaluating wound macroscopic and microscopic appearance. Material and method: Propolis extract were made using maceration technique using ethanol. Thirty Rattus norvegicus male rats were divided into six groups: three groups for treatment and three groups for control. Incision wound were made in the back and was infected with Methicillin ResistantStaphylococcus aureus (MRSA). Propolis was administered orally on daily basis. Wound excision for observation were done on day3, 5, and 7. The tissue were processed using haematoxylin-eosin (HE) staining for microscopic observation. The distance of normal tissue surrounding the wound were measured using image analysis program. Result: Macroscopic observation showed fewer visible wound in the propolis extract treated group. Measurement by microscopic observation on day 3 also demonstrated more narrow wound width in propolis extract treated group (405.75 110.11 μ) compared to the control group (806.20644.41μ). Conclusion: The result of this study suggests that propolis extract by oral administration promotes the healing of infected wound as shown within microscopic and microscopic observation.


Keywords


Animal model; propolis; wound healing; wound infection; Model hewan coba; penyembuhan luka; infeksi luka

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References


Al-Waili, N. Al-Ghamdi, A. Ansari, M. J. Al-Attal, Y. & Salom, K., 2012. Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus Aureus, Escherichia coli and Candida Albicans isolates in single and polymicrobial cultures. International Journal of Medical Sciences, 9(9): 793–800.

Almeida, E.B. Cordeiro, J. Karla, A. Lima, D. Oliveira, L. De, Oliveira, S., 2013. The incorporation of Brazilian propolis into collagen-based dressing films improves dermal burn healing. Journal of Ethnopharmacology, 147(2): 419–25.

Bin, L. & Wang, J.H.C., 2011. Fibroblasts and Myofibroblasts in Wound Healing: Force Generation and Measurement. J Tissue Viability, 20(4): 108–20.

Chitturi, R.T.Balasubramaniam, A.M. Parameswar, R.A. Kesavan, G. Haris, K.T.M. Mohideen, K., 2015. The Role of Myofibroblasts in Wound Healing, Contraction and its Clinical Implications in Cleft Palate Repair. Journal of International Oral Health, 7: 75–80.

Dakheel, K.H. Rahim, R.A. Neela, V.K. Al-Obaidi, J.R. Hun, T.G. Yusoff, K., 2016. Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion. BioMed Research International, 2016.

Diegelmann, R.F. & Evans, M.C., 2004. Wound healing: an overview of acute, fibrotic and delayed healing. Frontiers in Bioscience, January(1): 283–9.

Frykberg, R.G. & Banks, J., 2015. Challenges in the Treatment of Chronic Wounds. Advances in Wound Care, 4(9), 560–82.

Giacometti, A. Cirioni, O. Schimizzi, A.M. Prete, M.S.D.D’Errico, M.M. Petrelli, E. Scalise, G., 2000. Epidemiology and Microbiology of Surgical Wound Infections Epidemiology and Microbiology of Surgical Wound Infections. Journal of Clinical Microbiology, 38(2): 918–22.

Gonzalez, A.C.D.O. Costa, T.F. Andrade, Z.D.A. Medrado, A.R.A.P., 2016. Wound healing - A literature review. Anais Brasileiros de Dermatologia, 91(5): 614–20.

Gross, J.Farinelli, W. Sadow, P. Anderson, R. Bruns, R., 1995. On the mechanism of skin wound " contraction ": A granulation tissue " knockout " with a normal phenotype. Proc. Natl. Acad. Sci. USA, 92(June): 5982–6.

Hamouza, M.S. Rahman, M.F.A.E. Hafeez, M.A. Gerges, A., 2018. Egyptian fennel honey and / or propolis against MRSA harboring both mecA & icaA genes. International Journal of Complementary and Alternative Medicine, 11(3), 180–5.

Hampton, S., 2007. Bacteria and wound healing. Journal of Community Nursing, 21(10), 32–40.

Hozzein, W.N. Badr, G. Ghamdi, A.A.A. Sayed, A. Al-Waili, N.S. Garraud, O., 2015. Topical application of propolis enhances cutaneous wound healing by promoting TGF-beta/smad-mediated collagen production in a streptozotocin-induced type I diabetic mouse model. Cellular Physiology and Biochemistry, 37(3): 940–54.

Järbrink, K. Ni, G. Sönnergren, H. Schmidtchen, A. Pang, C. Bajpai, R.Car, J., 2016. Prevalence and incidence of chronic wounds and related complications : a protocol for a systematic review. Systematic Reviews, 5: 152.

Kuropatnicki, A.K.Szliszka, E.Krol, W., 2013. Historical aspects of propolis research in modern times. Evidence-Based Complementary and Alternative Medicine, 2013.

Landen, N.X.Li, D. Stable, M.,2016. Transition from inflammation to proliferation : a critical step during wound healing. Cell. Mol. LIfe Sci, 73: 3861–85.

Levinson, H., 2013. A Paradigm of Fibroblast Activation and Dermal Wound Contraction to Guide the Development of Therapies for Chronic Wounds and Pathologic Scars. Adv Wound Care (New Rochelle), 2(4): 149–59.

Lotfy, M.Badra, G. Burham, W. Alenzi, F.Q., 2006. Combined use of honey, bee propolis and myrrh in healing a deep, infected wound in a patient with diabetes mellitus. British Journal of Biomedical Science, 63(4): 171–3.

Machado, J.L. Assuncao, A.K.M.Silva, M.C.P.D. Assunc, M. Silva, A. Conceic, G.Berretta, A.A., 2012. Brazilian Green Propolis : Anti-Inflammatory Property by an Immunomodulatory Activity. Evidence-Based Complementary and Alternative Medicine, 2012: 1–10.

Nathwani, D. Davey, P.G. Marwick, C.A., 2010. MRSA: treating people with infection. Clinical Evidence, November: 1–18.

Ogawa, R., 2017. Keloid and hypertrophic scars are the result of chronic inflammation in the reticular dermis. International Journal of Molecular Sciences, 18(3).

Pastar, I.Stojadinovic, O.Yin, N.C. Ramirez, H. Nusbaum, A.G. Sawaya, A. Patel, S.B.Khalid, L. Isseroff, R.R.Tomic-Canic, M., 2014. Epithelialization in Wound Healing: A Comprehensive Review. Advances in Wound Care, 3(7), 445–64.

Pillai, S.I. Palsamy, P. Subramanian, S. Kandaswamy, M., 2010. Wound healing properties of Indian propolis studied on excision wound-induced rats. Pharmaceutical Biology, 48(11): 1198–206.

Schiaffino, S. & Reggiani, C., 2011. Fiber types in mammalian skeletal muscles. Physiol Rev, 91: 1447–1531.

Schindelin, J. Arganda-Carreras, I. Frise, E.Kaynig, V. Longair, M. Pietzsch, T. Preibisch, S.Rueden, C. Saalfeld, S.Schmid, B. Tinevez, J. White, D.J.Hartenstein, V.Eliceiri, K. Tomancak, P. Cardona, A. 2012. Fiji: an open-source platform for biological-image analysis. Nature Methods, 9(7): 676–682.

Sharpe, J.R. & Martin, Y., 2013. Strategies Demonstrating Efficacy in Reducing Wound Contraction In Vivo.Advances in Wound Care, 2(4): 167–175.

Sina, H. Ahoyo, T.A. Moussaoui, W.Keller, D. Bankolé, H.S. Barogui, Y. Stienstra, Y.Kotchoni, S.O. Baba-Moussa, L., 2013. Variability of antibiotic susceptibility and toxin production of Staphylococcus aureus strains isolated from skin, soft tissue, and bone related infections. BMC Microbiology, 13(1).

Siqueira, M.F. Li, J. Cheha, L. Desta, T. Chino, T., 2010. Impaired wound healing in mouse models of diabetes is mediated by TNF-α dysregulation and associated with enhanced activation of Forkhead boxO1 (FOXO1),. Diabetologia, 53: 378–388.

Toreti, V.C.Sato, H.H. Pastore, G.M. Park, Y.K., 2013. Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evidence-Based Complementary and Alternative Medicine, 2013.

Valente, M.J. Baltazar, A.F. Henrique, R. Estevinho, L.Carvalho, M., 2011. Biological activities of Portuguese propolis: Protection against free radical-induced erythrocyte damage and inhibition of human renal cancer cell growth in vitro. Food and Chemical Toxicology, 49(1): 86–92.

Velnar, T. Bailey, T. Smrkolj, V., 2009. The Wound Healing Process : an Overview of the Cellular and Molecular Mechanisms. The Journal of International Medical Research, 37(5): 1528–42.

Wagh, V.D., 2013. Propolis: A wonder bees product and its pharmacological potentials. Advances in Pharmacological Sciences, 2013.

Wojt czka, .D. K pa, M. Idzik, D.Ku ina, . Ka a a-Dzik, A.Dziedzic, A.Wa sik, .J., 2013. In vitro antimicrobial activity of ethanolic extract of polish propolis against biofilm forming staphylococcus epidermidis strains. Evidence-Based Complementary and Alternative Medicine, 2013.

Yeh, C. Chen, C. Leu, Y. Lin, M. Chiu, M. Wang, H., 2017. The effects of artocarpin on wound healing : in vitro and in vivo studies. Scientific Reports, 7(15599): 1–13.

Zhao, G. Usui, M.L. Lippman, S.I. James, G.A. Stewart, P.S. Fleckman, P. Olerud, J.E., 2013. Biofilms and Inflammation in Chronic Wounds. Advances in Wound Care, 2(7): 389–399.


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