Effect of Vasicine on Kidney Histomorphology in Streptozotocin-Induced Diabetic Wistar Rats
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Highlights
1. Justicia adhatoda leaves can be used as herbal medicine to treat diabetes and its associated conditions.
2. Vasicine in Justicia leaves helps protect kidney health in individuals with diabetes.
Background: Justicia adhatoda is a perennial shrub with various potential quinazoline alkaloids viz., vasicine, vasicinone, deoxyvasicine, vasicol, adhatodinine and vasicinol. Objective: The present study aimed to study the effect of vasicine, a quinazoline alkaloid on diabetic nephropathy induced with Streptozotocin in rats. Material and Method: The vasicine was extracted from the leaves of Justicia adhatoda. Thirty male Wistar rats were divided into four groups: Negative control group (plain water orally), Positive control (single dose of streptozotocin 55mg/ kg b wt dissolved in citrate buffer pH 4.5 intraperitoneally), treatment groups III and IV respectively (Glibenclamide, 5mg/kg body weight dissolved in 0.5% DMSO; Vasicine @v0.9 mg/kg dissolved in 0.5% DMSO respectively). The blood glucose was monitored every week). Serum analysis for creatinine and Blood Urea Nitrogen was done. On day 28, the kidneys were removed and prepared for routine histopathological observation using H&E staining. The histopathological changes of the kidneys complied with the biochemical values. Result: Vasicine treatment helped restoring the renal histoarchitecture and reduced serum creatinine and blood urea nitrogen. Conclusion: Vasicine extracted from Justicia adhatoda can help in protecting diabetic nephropathy.
Adeva-Andany, M. M., Adeva-Contreras, L., Fernández-Fernández, C., et al. 2023. Histological manifestations of diabetic kidney disease and its relationship with insulin resistance. Current Diabetes Reviews, 19(1). doi: 10.2174/1573399818666220328145046.
Ahmad, M., Abramovich, I., Agranovich, B., et al. 2022. Kidney proximal tubule GLUT2—more than meets the eye. Cells, 12(1): 94. doi: 10.3390/cells12010094.
Al-Ghaithi, F., El-Ridi, M. R., Adeghate, E., et al. 2004. Biochemical effects of Citrullus colocynthis in normal and diabetic rats. Molecular and Cellular Biochemistry, 261(1): 143–149. doi: 10.1023/B:MCBI.0000028749.63101.cc.
Alicic, R. Z., Rooney, M. T., Tuttle, K. R. 2017. Diabetic kidney disease. Clinical Journal of the American Society of Nephrology, 12(12): 2032–2045. doi: 10.2215/CJN.11491116.
Alotaibi, M. R., Fatani, A. J., Almnaizel, A. T., et al. 2019. In vivo assessment of combined effects of glibenclamide and losartan in diabetic rats. Medical Principles and Practice, 28(2): 178–185. doi: 10.1159/000496104.
D’souza, P. S., Holla, R., Swamy, G. 2021. Effect of Adhatoda zeylanica ethanolic extract on attenuated kidney in streptozotocin-induced diabetic rats. Journal of Health and Allied Sciences NU, 11(02): 073–079. doi: 10.1055/s-0040-1722801.
Dash, R. P., Chauhan, B. F., Anandjiwala, S., et al. 2010. Comparative pharmacokinetics profile of vasa swaras with vasicine and vasicinone. Chromatographia, 71(7–8): 609–615. doi: 10.1365/s10337-010-1517-x.
Dhankhar, S., Kaur, R., Ruhil, S., et al. 2011. A review on Justicia adhatoda: A potential source of natural medicine. African Journal of Plant Science, 5(11): 620–627. Available at: https://academicjournals.org/journal/AJPS/article-abstract/EFA667010942
Al Drees, A., Salah Khalil, M., Soliman, M. 2017. Histological and immunohistochemical basis of the effect of aminoguanidine on renal changes associated with hemorrhagic shock in a rat model. Acta Histochemica et Cytochemica, 50(1): 11–19. doi: 10.1267/ahc.16025.
Elkader, S. A., Elmogy, M., El-Sappagh, S., et al. 2018. A framework for chronic kidney disease diagnosis based on case based reasoning. International Journal of Advanced Computer Research, 8(35): 59–71. doi: 10.19101/IJACR 2018.834003.
Furman, B. L. 2015. Streptozotocin‐induced diabetic models in mice and rats. Current Protocols in Pharmacology, 70(1). doi: 10.1002/0471141755.ph0547s70.
Garcia, F. A. O., Pinto, S. F., Cavalcante, A. F., et al. 2014. Pentoxifylline decreases glycemia levels and TNF-alpha, iNOS and COX-2 expressions in diabetic rat pancreas. SpringerPlus, 3(1): 283. doi: 10.1186/2193-1801-3-283.
Ghezzi, C., Loo, D. D. F., Wright, E. M. 2018. Physiology of renal glucose handling via SGLT1, SGLT2 and GLUT2. Diabetologia, 61(10): 2087–2097. doi: 10.1007/s00125-018-4656-5.
Jensen, P. K., Steven, K., Blæhr, H., et al. 1986. Effects of indomethacin on glomerular hemodynamics in experimental diabetes. Kidney International, 29(2): 490–495. doi: 10.1038/ki.1986.26.
Khandelwal, P., Wadhwani, B. D., Rao, R. S., et al. 2024. Exploring the pharmacological and chemical aspects of pyrrolo-quinazoline derivatives in Adhatoda vasica. Heliyon, 10(4): e25727. doi: 10.1016/j.heliyon.2024.e25727.
Kim, H. 2014. Analysis of variance (ANOVA) comparing means of more than two groups. Restorative Dentistry & Endodontics, 39(1):74. doi: 10.5395/rde.2014.39.1.74.
Kitada, M., Ogura, Y., Koya, D. 2016. Rodent models of diabetic nephropathy: Their utility and limitations. International Journal of Nephrology and Renovascular Disease, 9: 279–290. doi: 10.2147/IJNRD.S103784.
Matsuyama, S., Karim, M. R., Izawa, T., et al. 2018. Immunohistochemical analyses of the kinetics and distribution of macrophages in the developing rat kidney. Journal of Toxicologic Pathology, 31(3): 207–212. doi: 10.1293/tox.2018-0002.
Mir, S. H., Baqui, A., Bhagat, R. C., et al. 2008. Biochemical and histomorphological study of streptozotocin-induced diabetes mellitus in rabbits’, Pakistan Journal of Nutrition, 7(2): 359–364. Available at: https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=5ddd04a9fb3caf8a05bdebb198993307149f9df0
Pourghasem, M., Shafi, H., Babazadeh, Z. 2015. Histological changes of kidney in diabetic nephropathy. Caspian Journal of Internal Medicine, 6(3): 120–7. Available at: http://www.ncbi.nlm.nih.gov/pubmed/26644877
Rao, V. R. A. B. V.,Tan, S. H., Candasamy, M., et al. 2019. Diabetic nephropathy: An update on pathogenesis and drug development. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 13(1): 754–762. doi: 10.1016/j.dsx.2018.11.054.
Ravali, K., Kumary, U., Sarathchandra, G., et al. 2024. Vasicine a quinazoline alkaloid from Justicia adhatoda L.: Its antioxidant property. International Journal of Advanced Biochemistry Research, 8(1S): 729–731. doi: 10.33545/26174693.2024.v8.i1Sj.415.
Samsu, N. 2021. Diabetic nephropathy: Challenges in pathogenesis, diagnosis, and treatment. BioMed Research International. Edited by M. I. Bellini, 2021: 1–17. doi: 10.1155/2021/1497449.
Sharma, N., Sharma, V., Manikyam, H., et al. 2016. Ergot alkaloids: A review on therapeutic applications. European Journal of Medicinal Plants, 14(3): 1–17. doi: 10.9734/EJMP/2016/25975.
Singh, R., Tiwari, A. 2016. Adhatoda vasica : A Miracle and Boon for Asthmatic people-A Review. Research Journal of Pharmacognosy and Phytochemistry, 8(4): 242. doi: 10.5958/0975-4385.2016.00036.4.
SPSS Inc. (2007). SPSS for Windows, version 15.0. Chicago, SPSS Inc.Available at: https://www.ibm.com/support/pages/downloading-ibm-spss-modeler-150
Sugimoto, H., Grahovac, G., Zeisberg, M., et al. 2007. Renal fibrosis and glomerulosclerosis in a new mouse model of diabetic nephropathy and its regression by bone morphogenic protein-7 and advanced glycation end product inhibitors. Diabetes, 56(7): 1825–1833. doi: 10.2337/db06-1226.
Vrhovac, I., Balen Eror, D., Klessen, D., et al. 2015. Localizations of Na+-d-glucose cotransporters SGLT1 and SGLT2 in human kidney and of SGLT1 in human small intestine, liver, lung, and heart. Pflügers Archiv - European Journal of Physiology, 467(9): 1881–1898. doi: 10.1007/s00424-014-1619-7.
Yadav, S., Yadav, V. K. 2018. Ethnomedicinal value and pharmacognosy of the member of Acanthaceae: Adhatoda vasica (Linn.). Asian Pacific Journal of Health Sciences, 5(2): 40–43. doi: 10.21276/apjhs.2018.5.2.10.
Yassin, M. M., Ashour, A. R. A., Elyazji, N. R. 2004. Alterations in body weight, protein profile, non-protein nitrogen constituents and kidney structure in diabetic rats under glibenclamide treatment. IUG Journal of Natural Studies, 12(1): 37-54. Available at: https://journals.iugaza.edu.ps/index.php/IUGNS/article/view/243/0.
Yu, S., Shang, P. 2014. A review of bioeffects of static magnetic field on rodent models. Progress in Biophysics and Molecular Biology, 114(1): 14–24. doi: 10.1016/j.pbiomolbio.2013.11.002.
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