The effect of docosahexaenoic acid (DHA) supplementation on total antioxidant capacity (TAC), superoxide dismutase (SOD), and interleukin-6 levels in underweight pregnant women
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HIGHLIGHTS
- Underweight pregnant women, face an imbalance in energy and protein intake.
- TAC, SOD, and IL6 with administration of DHA to pregnant women with chronic energy deficiency in the third trimester provide benefits.
ABSTRACT
Objective: Underweight pregnant women face oxidative stress and inflammation, increasing their risk of intrauterine growth restriction (IUGR) and preterm birth. This study investigates the effects of DHA supplementation on Total Antioxidant Capacity (TAC), Superoxide Dismutase (SOD), and Interleukin-6 (IL-6) in underweight pregnant women, along with the correlation between DHA and these markers.
Materials and Methods: This experimental pre-test/post-test study focused on underweight pregnant women in the Made District, Surabaya, Indonesia. Eligible participants were in their second or third trimester, had a BMI below 18.5, and were taking DHA regularly. Exclusion criteria included early pregnancy (gestational age < 14 weeks), BMI above 18.5, irregular DHA intake, and withdrawal from the study. The study ran from July to December 2023, using non-probability sampling to select participants. Blood samples were collected before and after two months of DHA supplementation.
Results: Following the intervention, TAC levels demonstrated a noteworthy increase (p < 0.05). SOD levels also exhibited a significant difference (p <0.05), and IL-6 levels showed a significant change (p < 0.05). A strong and positive correlation (r = 0.718) was observed between the increased TAC and SOD levels. DHA influenced both TAC and IL-6, with a significant relationship between TAC and IL-6 (p < 0.01). Furthermore, elevated SOD levels were associated with a decrease in IL-6 levels (p < 0.01). The correlation coefficient value of 0.718 between changes in SOD and TAC indicated a robust positive correlation.
Conclusion: The findings suggest that DHA supplementation in underweight pregnant women positively affects oxidative stress and inflammation markers, improving TAC, SOD, and IL-6 levels.
Burnie R, Golob E, Clarke S. Pregnancy in underweight women: implications, management and outcomes. The Obstetrician & Gynaecologist. 2022;24(1):50–7. doi: 10.1111/tog.12792.
Henderi H, Siahaan SCPT, Kusumah IP, et al. Correlation of vitamin D with ferritin in pregnant mothers chronis energy deficiency of the second trimester. Vol. 17, Berkala Kedokteran. Jurnal Kedokteran & Kesehatan. 2021;17(2):143-50. doi: 10.20527/jbk.v17i2.11675.
Aji AS, Lipoeto NI, Yusrawati Y, et al. Association between pre-pregnancy body mass index and gestational weight gain on pregnancy outcomes: a cohort study in Indonesian pregnant women. BMC Pregnancy Childbirth. 2022;22(1):492. doi: 10.1186/s12884-022-04815-8. PMID: 35705902; PMCID: PMC9202216.
Hussain T, Murtaza G, Metwally E, et al. The role of oxidative stress and antioxidant balance in pregnancy. Mediators Inflamm. 2021;2021:996 2860. doi: 10.1155/2021/9962860. PMID: 34616 234; PMCID: PMC8490076.
Saenz de Viteri M, Hernandez M, Bilbao-Malavé V, et al. A higher proportion of eicosapentaenoic acid (EPA) when combined with docosahexaenoic acid (DHA) in Omega-3 dietary supplements provides higher antioxidant effects in human retinal cells. Antioxidants (Basel). 2020;9(9):828. doi: 10.3390/antiox9090828. PMID: 32899655; PMCID: PMC7555332.
Siahaan SCPT, Henderi H, Sudibjo, et al. Intervensi ibu hamil dengan kurang energi kalori melalui suplementasi mikronutrien di Surabaya tahun 2019. Majalah Kedokteran Andalas. 2021;44(1):17–27. doi: 10.25077/mka.v44.i1.p17-27.2021.
Li G, Li Y, Xiao B, et al. Antioxidant activity of docosahexaenoic acid (DHA) and its regulatory roles in mitochondria. J Agric Food Chem. 2021;69(5):1647-55. doi: 10.1021/acs.jafc.0c07751. Epub 2021 Jan 26. PMID: 33497204.
Lafuente M, Rodríguez González-Herrero ME, Romeo Villadóniga S, et al. Antioxidant activity and neuroprotective role of docosahexaenoic acid (DHA) supplementation in eye diseases that can lead to blindness: A narrative review. Antioxidants (Basel). 2021;10(3):386. doi: 10.3390/antiox1003 0386. PMID: 33807538; PMCID: PMC8000043.
Lafuente M, Ortín L, Argente M, et al. Three-year outcomes in a randomized single-blind controlled trial of intravitreal ranibizumab and oral supplementation with docosahexaenoic acid and antioxidants for diabetic macular edema. Retina. 2019;39(6):1083-90. doi: 10.1097/IAE.000000000 0002114. PMID: 29474306; PMCID: PMC655 3973.
Martínez-Soto JC, Domingo JC, Cordobilla B, et al. Dietary supplementation with docosahexaenoic acid (DHA) improves seminal antioxidant status and decreases sperm DNA fragmentation. Syst Biol Reprod Med. 2016;62(6):387-95. doi: 10.1080/ 19396368.2016.1246623. Epub 2016 Oct 28. PMID: 27792396.
Sanyoto DD, Asnawati A, Triawanti T. Effect of DHA supplementation on the MDA and SOD levels in protein malnourished rats. Journal of Physics: Conference Series. Institute of Physics Publishing. 2019. doi: 10.1088/1742-6596/1374/1/012036.
Priscilla P, Siahaan SCPT, Santosa RI, et al. Correlation between DHA (docosahexaenoic acid) supplementations and SOD (Superoxide Dismutase) on underweight pregnant. International Journal of Medical and Pharmaceutical Research. 2023;(6):172-7. doi: 10.5281/zenodo.10448124.
Innes JK, Calder PC. Omega-6 fatty acids and inflammation. Prostaglandins Leukot Essent Fatty Acids. 2018;132:41-8. doi: 10.1016/j.plefa.2018.03. 004. Epub 2018 Mar 22. PMID: 29610056.
Gita N, Wiranthika P, Siahaan SCPT, et al. Correlation of DHA (docosahexanoic acid) supplementation to underweight pregnant women regarding the inflammatory mediator IL-6 (Interleukin-6). International Journal of Medical and Pharmaceutical Research. 2023;(6):178-83. doi: 10.5281/zenodo.10448149.
Sepidarkish M, Akbari-Fakhrabadi M, Daneshzad E, et al. Effect of omega-3 fatty acid plus vitamin E Co-Supplementation on oxidative stress para-meters: A systematic review and meta-analysis. Clin Nutr. 2020;39(4):1019-25. doi: 10.1016/j.clnu. 2019.05.004. Epub 2019 May 10. PMID: 3112 8941.
Arab Sadeghabadi Z, Abbasalipourkabir R, Mohseni R, et al. Investigation of oxidative stress markers and antioxidant enzymes activity in newly diagnosed type 2 diabetes patients and healthy subjects, association with IL-6 level. J Diabetes Metab Disord. 2019;18(2):437-43. doi: 10.1007/ s40200-019-00437-8. PMID: 31890669; PMCID: PMC6915251.
Peris-Martínez C, Piá-Ludeña JV, Rog-Revert MJ, et al. Antioxidant and anti-inflammatory effects of oral supplementation with a highly-concentrated Docosahexaenoic Acid (DHA) triglyceride in patients with keratoconus: A randomized controlled preliminary study. Nutrients. 2023;15(5):1300. doi: 10.3390/nu15051300. PMID: 36904299; PMCID: PMC10005296.
Amirkhizi F, Hamedi-Shahraki S, Rahimlou M. Dietary total antioxidant capacity is associated with lower disease severity and inflammatory and oxidative stress biomarkers in patients with knee osteoarthritis. J Health Popul Nutr. 2023;42(1):104. doi: 10.1186/s41043-023-00450-x. PMID: 3777 0996; PMCID: PMC10540397.
Kumboyono K, Chomsy IN, Hakim AK, et al. Detection of Vascular Inflammation and Oxidative Stress by Cotinine in Smokers: Measured Through Interleukin-6 and Superoxide Dismutase. Int J Gen Med. 2022;15:7319-28. doi: 10.2147/IJGM. S367 125. PMID: 36147199; PMCID: PMC9489 220.
Mridha MK, Matias SL, Chaparro CM, et al. Lipid-based nutrient supplements for pregnant women reduce newborn stunting in a cluster-randomized controlled effectiveness trial in Bangladesh. Am J Clin Nutr. 2016;103(1):236-49. doi: 10.3945/ajcn. 115.111336. Epub 2015 Nov 25. PMID: 26607935; PMCID: PMC6443293.
Losano JDA, Angrimani DSR, Rui BR, et al. The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls. Zygote. 2018; 26(3):199-206. doi: 10.1017/S0967199418000096. Epub 2018 May 21. PMID: 29781410.
Garrel C, Alessandri JM, Guesnet P, et al. Omega-3 fatty acids enhance mitochondrial superoxide dismutase activity in rat organs during post-natal development. Int J Biochem Cell Biol. 2012;44 (1):123-31. doi: 10.1016/j.biocel.2011.10.007. Epub 2011 Oct 30. PMID: 22062949.
Borgonovi SM, Iametti S, Di Nunzio M. Docosahexaenoic acid as master regulator of cellular antioxidant defenses: A systematic review. Antioxidants (Basel). 2023;12(6):1283. doi: 10.3390/antiox12061283. PMID: 37372014; PMCID: PMC10295041.
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