Moderate Intensity of Physical Exercise increased Β (Beta) Cell and Size of Langerhans Islets in Streptozotocin Induced Diabetes Mellitus Rats

Sarah M Nurdin, Nuniek Nugraheni, Mei Wulan

= http://dx.doi.org/10.20473/spmrj.v1i2.16176
Abstract views = 988 times | downloads = 619 times

Abstract


Background: The death of β cells Langerhans islets in Diabetes Mellitus (DM) can cause  loss of Langerhans islet function and worsen the progression of DM. Physical exercise plays a major part in DM treatment.

Aim: to observe the effect of moderate intensity exercise to β (beta) cell numbers and Langerhans islets area size in Streptozotocin (STZ) induced diabetes in rats.

Methods: Thirty adult male Wistar rats (Rattusnorvegicus) divided into 3, Group 1 as the control, Group 2 received 35 mg/kg streptozotocin induction treatment, Group 3 received 35 mg/kg streptozotocin induction and physical exercise, swimming, with moderate intensity 70% from the swimming maximal ability, 9% of body weight load, 4 times a week for 4 weeks. Datas collected were in the form of histopathology slide of pancreatic tissue after receiving treatment for 28 days.

Results: There are significant differences of β-cell pancreas number between group K1 and K2 (p<0,001), group K2 and to K3 (p<0,001). No significant difference between group K1 and K3 (p=0,102). The Langerhans islets area sizes of pancreas tissue between group K1, K2, and K3 are significantly different (p<0,001).

Conclusion: This study shows moderate-intensity physical exercise can increase the number of β cell and average area size of Langerhans islets. The effect of physical exercise depends on the intensity of exercise and the capacity of pancreatic function left of the diabetic.


Keywords


diabetes mellitus; physical exercise; β-cell pancreas; Langerhans islet morphology

Full Text:

PDF

References


American Diabetes Assosiation (ADA). Diagnosis and classification of diabetes mellitus. Diabetes Care. 2012;35(1): 64-71.

Weir GC, Bonner-Weir S. Five stages of evolving β-cell dysfunction during progression to diabetes. Diabetes. 2004;53(3):S16-20.

Coskun O, Ocakci A, Bayraktaroglu T, Kanter M. Exercise training prevents and protects streptozotocin-induced oxidative stress and B cell damage in rat pancreas. Tohoku J Exp Med. 2004;203:145-54.

Colombo M, Gregersena S, Kruhoefferb M, Aggera A, Xiaoa J, et al. Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by highdensity oligonucleotide microarray analysis. Met Clin Exp. 2005;54:1571-81.

Lemos ET, Nunes S, Texiera F, Reis F. Regular physical exercise training assist in preventing type 2 diabetes development: Focus on antioxidant and anti-inflamatory properties. Cardiovascular Diabetology. 2011;10(12): 1-15

Zoppi C, Sabrina G, Carlos AB, Magalhaes CE. Exercise induced pancreatitic islet adaptations in health and disease. Islets of Langerhans, 2 ed. Dordrecht: Springers Science+Bussiness Media; 2013.

Sobrevilla VJ, Villa BVD, Aguilar CN, Ramos RR, Avila VE, Sepulveda SE et al.. Effect of varying dose and administration of streptozotocin on blood sugar in male CD1 mice. Proc West Pharmacol Soc. 2011;54:5-9.

Király MA, Bates HE, Kaniuk NA, Yue JT, Brumell JH, et al. Swim training prevents hyperglycemia in ZDF rats: mechanisms involved in the partial maintenance of β-cell function. Am J Physiol Endocrinol Metab. 2008;94:271-83.

Eleazu CO, Eleazu KC, Shukwuma S, et al. Review of the mechanism of cell death resulting from Streptozotocin challenge in experimental animals, its practical use and potensial risk to humans. Journal of diabetes & metabolic disorders. 2013;12:60.

Leonardi O, Mints G, Hussain MA. Β-cell apoptosis in the pathogenesis of human type 2 diabetes mellitus. European Journal of Endocrinology. 2003;149:99–102.

Goodwin ML. Review article, blood glucose regulation during prolonged, submaximal, continuous exercise: A guide for clinicians. J Diabetes Sci Technol. 2010;4(3):694-705.

Mc Ardle, Pattwell D, Vasilaki, et al. Contractile activity – induced oxidative stress: Cellular origin and adaptive responses, Am J Physiol Cell Physiol. 2001; 280(3):C621-7.

Baqarizky F. Studi awal: gambaran histopatologik pankreas, hepar dan ginjal tikus diabetes mellitus yang diinduksi Sterptozotocin dengan pewarnaan hematosiklin eosin. Jakarta: UIN Syarif Hidayatullah; 2015.

Birben E, Sahiner UM, Sackesen C, Erzurum S. Oxidative stress and antioksidant defense. World Allergy Organ Journal. 2012;5(1):9-19.

Cardozo AK, Kruhoffer M, Leeman R, Orntoft T, Eizirik D. Identification of novel cytokine-induced genes in pancreatic β-cells by high-density oligonucleotide arrays. Diabetes. 2001;50:909-20.

Harahap AS, Herman RB, et al. Gambaran glukosa darah setelah latihan fisik pada tikus Wistar diabetes mellitus yang diinduksi Aloksan. Jurnal Kesehatan Andalas. 2015;4(1):23-9.

Kregel K, Toth L, Henriksen E, Ra’anan A, et al. Resource Book For The Design Of Animal Exercise Protocols. US: American Physiology Society; 2006. p34-41.

Abeeleh MA, Ismail ZB, Alzaben KR, Abu-Halaweh SA, Al-Essa MK, Abuabeeleh J, et al. Induction of diabetes mellitus in rats using intraperitoneal Streptozotocin X: A comparation between 2 strains in rats. Eur J Sci Res. 2009;32(3):398-40.

Adeghate E, Ponery A. Diabetes mellitus influences the degree of colocalization of calcitonin gene-related peptide with insulin and somatostatin in the rat pancreas. J Pancreas. 2004;29(4):311-9.

Huang HH, Farmer K, Windscheffel J, Yost K, Power M, Wright DE, et al. Exercise increases insulin content and basal secretion in pancreatic islets in type 1 diabetic mice. Exp Diabetes Res. 2011;2011:481427

Jung JY, Lim Y, Moon MS, Kim JY, Kwon O. Onion peel extracts ameliorate hyperglycemia and insulin resistance in high fat diet/ Streptozotocin-induced diabetic rats. Nutrition and Metabolism. 2011;8(1):18.

Halliwell, B, and Gutteridge ,J, 1999, Free radicals in biology and Mdicine (3rd ed). New York : Oxford University Press.

Guzel NA, Hazard S, Erbas D. Effect of difference resistance exercise protocol on nitric oxide, lipid peroxidation and creatine kinase activity in sedentary males. Journal of Sports Science and Medicine. 2007;6:417-22.


Refbacks

  • There are currently no refbacks.


Creative Commons License
SPMRJ by  Unair is licensed under a Creative Commons Attribution 4.0 International License.

 

        

View SPMRJ Stats