Screening and Identification of Fibrinolitic Bacteria from Tempeh

Tempe is a food that is well known by indonesian people. The purpose of this study was to obtain bacteria with the largest fibrinolytic activity from Tempe. This research sample was obtained from four different markets in Surabaya area. Proteolytic activity of bacteria is examined using Skim Milk Agar media. Followed by fibrinolytic examination on the fibrin plate media. Identification of bacteria is carried out by gram staining method and 16S rRNA method. The sample is prepared by mixing the sample with a normal saline solution until 10-7 dilution is obtained. Then the bacterial suspension is spread on SMA media then incubated at 37°C for 24 hours. The bacterial suspension is spread onSMA media then incubated at 37°C for 24 hours. The selection of fibrinolytic protease producing bacteria was carried out using Skim Milk Agar (SMA) and Fibrin Agar (FA) are characterized by the presence of clear zones around them. Bacteria that produce fibrinolytic activity are then cultured in the Nutrrient Agar medium. The bacteria was then tested for fibrinolytic activity on the Fibrin Plate media in incubation at 37°C for 24 hours.  Positive bacteria are calculated by measuring the diameter of clear zones. Bacteria with sample code T2.2 produce the largest fibrinolytic index. T2.2 bacteria are then microscopically characterized, macroscopically, and bacterial isolates showing fibrinolytic activities were identified using Polymerase Chain Reaction (PCR) targeting their 16S rRNA gene. T2.2 bacteria is thought to be Stenotrophomonas maltophilia with a percentage similarity 96%.