Optimasi Produksi Fibrinolitik oleh Bacillus megaterium BM 9.1 menggunakan media Limbah Pertanian sebagai sumber karbon
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The study of effect agro recidues carbon sources on fibrinolytic enzyme production by Bacillus megaterium BM 9.1 was done by using fibrin plate method. Bacillus megaterium BM 9.1 grown on agro residues medium with solid state fermentation. The fibrinolytic enzymes activity is declared by fibrinolytic index. Fibrinolytics index is the comparisson between clear zones and wall diameters. From various carbon sources tested, Bacillus megaterium BM 9.1 which is grown in banana peel, pineaple skin, corn husk, rice bran and corn cob could produce fibrinolytic enzymes. Optimum fibrinolytic activities found in a nutrient agar and banana peel medium at fibrinolytic index 4,95 ± 0,08 (p<0,05). The comparisson fibrinolytic index between Bacillus megaterium BM 9.1 which grown in combination nutrient agar, banana peel and nutrien agar standart is 117,%. Banana peel concentration optimation was done at concentration of 0,5 %, 1 %, 2 % and 4 % and the fibrinolytic index was found respectively according to 4,26 ± 0,11 ; 4,32 ± 0,03 ; 4,61 ± 0,03; and 4,35 ± 0,05. The optimum fibrinolytic activity was found in banana peel on consentration 2 %.
Al-Abdalall, A. H., & Al-Khaldi, E. M. (2016). Production of alkaline proteases by alkalophilic Bacillus subtilis during recycling animal and plant wastes. African Journal of Biotechnology, 15(47), 2698–2702.
Atlas, R. M. (n.d.). Handbook of MICROBIOLOGICAL MEDIA Fourth Edition.
Bhargav, S.; Panda, B. P.; Ali,. M.; Javed, S. (2008). Solid-state fermentation systems-an overview. Critical reviews in biotechnology, 25(1–2), 1–30.
Fakhri, C. H. (2015). Skrining bakteri penghasil enzim fibrinolitik asal perairan pantai ekowisata mangrove wonorejo kota surabaya. universitas airlangga.
Glaser, A. N., & Nikaido, H. (2007). Microbial biotechnology. Fundamentals of Applied Microbiology, Second Edition. (S. Edition, Ed.)Journal Of Bacteriology (Second.). New York: Published in the United States of America by Cambridge University Press, New York.
Kotb, E. (2015). Purification and Partial Characterization of Serine Fibrinolytic Enzyme from Bacillus megaterium KSK 07 Isolated from Kishk , a Traditional Egyptian Fermented Food 1, 51(1), 34–43.
elson, D. L., & Cox, M. M. (2008). Lehninger PRINCIPLE OF BIOCHEMISTRY (5th editio.).
Peng, Y., Huang, Q., Zhang, R. H., & Zhang, Y. Z. (2003). Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 134(1), 45–52.
Prod, J. N., Resour, P., Arulanantham, R., Pathmanathan, S., & Ravimannan, N. (2012). Alternative culture media for bacterial growth using different formulation of protein sources, 2(6), 697–700.
Singh Nee Nigam, P., & Pandey, A. (2009). Biotechnology for agro-industrial residues utilisation: Utilisation of agro-residues. Biotechnology for Agro-Industrial Residues Utilisation: Utilisation of Agro-Residues.
Stanbury, P. F., Whitaker, A., & Hall, S. J. (2013). Principles of fermentation technology (Second Ed., Vol. 53). Butterworth-Heinemann An imprint of Elsevier Science 200 Wheeler Road, Burlington MA 01803.
Vijayaraghavan, P, & Vincent, S. G. P. (2015). A low cost fermentation medium for potential fi brinolytic enzyme production by a newly isolated marine bacterium , Shewanella sp . Biotechnology Reports, 7, 135–142. Elsevier B.V.
Vijayaraghavan, Ponnuswamy, Vincent, S. G. P., Arasu, M. V., & Al-Dhabi, N. A. (2016). Bioconversion of agro-industrial wastes for the production of fibrinolytic enzyme from Bacillus Halodurans IND18: Purification and biochemical characterization. Electronic Journal of Biotechnology, 20, 1–8. Elsevier B.V.
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