Daya Antibakteri EkstrakMeniran (Phyllanthus niruri linn) Terhadap BakteriEnterococcus faecalis (Antibacterial Activity Of Phyllanthus niruri linn Extract Against Enterococcus faecalis Bacteria)
Downloads
Background. Enterococcus faecalis is an anaerobic facultative gram-positive bacteria which contribute to the failure of root canal treatment with the number of prevalence 24% to 77%. At the preparation stage, a material for irrigation which has antibacterial activity to Enterococcus faecalis is needed. Phyllantus niruri linn is one of herbal medicament which is potential as antibacterial agent as it contains active antibacterial chemical-compound. Purpose. The purpose of the study is to identify Minimum Inhibitory Concentration and Minimum Bactericidal Concentrationof Phyllantus niruri linn against Enterococcus faecalis.Method. The research method used is laboratory experimental. Enterococcus faecalis ATCC 29212 was suspended into several concentration of Phyllantus niruri linn extract from dilution method on BHIB medium. Each tube was incubated for 24 hours. Then, each tube was subcultured to Nutrient agar medium using spreader in a petridish. Each petridish was incubated for 24 hours and the growth of the colony was manually calculated using CFU/ml unit. Result. At the concentration of 6.25%, Phyllanthus niruri linn was able to inhibit the growth of Enterococcus faecalis as 90% and there was no bacteria at the concentration of 12.5%. Conclusion. 6.25% concentration of Phyllanthus niruri linn extract was Minimum Inhibitory Concentration and 12.5% concentration was Minimum Bactericidal Concentration to Enterococcus faecalis.
Mulyawati E. 2011. Peran Bahan Disinfekasi Pada Perawatan Saluran Akar. Majalah Kedokteran Gigi. Vol 18. No.2. Pp. 205-9.
Grossman LI, Oliet S, Rio CED. 2010. Ilmu endodontik dalam praktek. Edisi 12.Jakarta: EGC. Pp. 25-9.
Walton ER, Torabinejad M. 2008. Prinsip dan Praktik Ilmu Endodonsia. Ed 3. Jakarta: EGC. Pp. 243-47.
Wang QQ, Zhang Fc, Chu HC, Zhu FX. 2012. Prevalence of Enterococcus FaecalisIn saliva and filled root canals of teeth associated with apical periodontitis. International Journal of Oral Science. Vol 4. Pp. 19-3.
Shahani MN, Reddy VVS, 2011. Comparison of antimicrobial substantivity of root canal irrigants in instrumented root canals up to72 h: an in vitro study.J Indian Soc Pedod Prev Dentistry. Pp. 29.
Narayanan LL and Vaishanavi C, 2010. Endodontic Microbiology. India: Department of Conservative Dentistry. J Conserv Dent. Vol. 4 No. 13. Pp. 233-39.
Stuart CH, Scott A. Schwartz, Besoon TJ, and Christopher BO. 2006. Enterococcus faecalis: Its Role in Root Canal Treatment Failure and Current Concepts in Retreatment. Review Article.Vol. 32. No. 2. Pp. 93-8.. Ed 3. Jakarta: EGC. Pp. 243-47.
Fitriyah. 2013. Obat Herbal Antibakteri ala Tanaman Binahong.Jurnal KesMaSka. Vol. 23. No. 34.Pp. 20-5.
Wibowo S. 2013. Herbal ajaib. Jakarta Pusat: Pustaka Makmur. Pp. 32-4.
Munfaati NP, Ratnasari E, Trimulyono G. 2015. In Vitro Antibacterial Compound Activity of Meniran Herbs (Phillanthus niruri) Extract on the Frowth of Shigella Dysenteriae Bacteria.LenteraBio J. Vol. 4. No. 1.Pp. 64-1.
Kardinan A, Kusuma FR. 2004. Meniran penambah daya tahan tubuh alami. Jakarta: Agromedia Pustaka. Pp. 6-18.
Shanmugam B, Shanmugam RK, Ravi Sahukari, Subbaiah VG, Mallikarjuna K, Reddy SK. 2014. Antibacterial Activity and Phytochemical Screening of Phyllanthus Niruri Linn in Ethanolic, Methanolic and Aqueous Extracts. Inc J. Pharm, Sci. Res.Vol. 27. No. 2. Pp. 85-9.
Obiagwu IN, Okechalu OB, Njoku MO. 2011. Studies on Antibacterial Effect of the Leaves OfPhyllanthus niruri on Some Enteric Pathogens. Nigerian Journal of Biotechnology. Vol. 23. Pp. 22-2.
Sudarno, Setiorini AF, Suprapto H. 2011. Effectivity Of Meniran (Phyllanthus Niruri L) Extacts as Edwardsiella tarda Antibacterial According In vitro. Jurnal Ilmiah perikanan dan Kelautan. Vol. 3. No. 1.Pp.103-8.
Kunaepah U, 2008. The effect of Fermentation Duration and Glucose Concentration on Antibacterial Activity, Total Polyphenol and Chemical Quality of Kidney Bean Milk Kefir. Jurnal Universitas Diponegoro. Pp. 25-7.
CDJ by Unair is licensed under a Creative Commons Attribution 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions