The Antibacterial Potential of Tannin from Mangosteen pericarp Extract against Lactobacillus casei
Downloads
Background: Deep dentinal caries cannot defect the vitality of dental pulp. The microbial populations involved in dental caries are known to be highly complex and variable. Lactobacillus casei is one of the most common carious dentine bacteria. Tannin contained in mangosteen pericarp extract is known to have an antibacterial potency. Purpose. This study aims to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration of tannin from mangosteen pericarp extract against Lactobacillus casei. Method. This study is an experimental laboratory with a post-test only control group design. Tannin from mangosteen was extracted by 96% ethanol. Lactobacillus casei was diluted into several concentrations using the serial dilution method in the Brain Heart Infusion Broth (BHIB) medium and inserted in several reaction tubes. Every reaction tube was incubated for 24 hours. After being incubated, each concentration was taken and swabbed into Mueller Hinton in the petri dish. Then, every petri dish was incubated for 24 hours and colonies' growth was counted manually in the Colony Forming Unit (CFU). Result. Bacterial colonies' growth at a concentration of 1.56% was 90% less than the positive control group and there was no bacterial colonies growth at the concentration of 3.12%. Conclusion. Tanin from mangosteen pericarp extract has an antibacterial effect against Lactobacillus casei. The MIC of tannin from mangosteen pericarp extract against Lactobacillus casei was at 1.56% and MBC was at 3.12%.
Kidd, Edwina. 2005. Essential of Dental Caries. Third edition. New York : Oxford University Press
Roberson, Theodore, Heymann, Harald. 2012. Sturdevant's Art and science of operative dentistry. USA : Mosby, Inc
Love, Robert. 2004. Invasion of Dentinal Tubules by Root Canal Bacteria. Endodontic Topics, 9, 52-65. Blackwell Munkgaard
Byun Roy, Nadkarni M.A, Chhour Kim-Ly, Martin F.E, Jacques N.A, and Neil Hunter. 2004. Quantitative Analysis of Diverse Lactobacillus Species Present in Advanced Dental Caries. Journal Of Clinical Microbiology, Vol. 42, No. 7, p. 3132
Kaplowitz GJ, Cortell M. Chlorhexidine : A Multifunctional antimicrobial Drug. RDH, 2007;27:1-7
Pradipta I. S., Nikodemus T. W. dan Susilawati Y. 2004. Isolasi dan Identifikasi Senyawa Golongan Xanton dari Kulit Buah Manggis (Garcinia mangostana L.) hlm 64-77
Smullen J, Koutsou GA, Foster HA, Zumbé A, Storey DM. The antibacterial activity of plant extracts containing polyphenols against Streptococcus mutans. Caries Res. 2007;41(5):342-9.
CDJ by Unair is licensed under a Creative Commons Attribution 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions