Main Article Content


Tuberculosis in children is often a systemic complication because of their imperfect cellular immune system. ESAT-6, CFP-10, and MPT-64 are small molecular dominant antigens secreted by Mtb into the blood and filtered by the kidneys into urine. The high proportion of children with TB cases according to the Ministry of Health of the Republic of Indonesia in 2013 was 7.9%-12%. Diagnis is difficult to establish with sputum and blood samples. Therefore, it is necessary to support the diagnosis using urine samples with ICT rapid test. The purpose of this study was to determine the examination of Mtb ESAT-6, CFP-10, and MPT-64 antigens in the urine of pediatric TB patients with ICT to support the diagnosis. The method used was analytic observation with case control design. The results of the study showed that 32 of the urine of children with TB who were examined with ICT, 22 showed ESAT-6, CFP-10, and MPT-64 positive and 10 negative. Whereas, from 32 urine of healthy children, 25 were negative and 7 were positive. Analysis results showed 95% confidence (CI), p = 0.0002 (p <0.05), 78% specificity and 68.8% sensitivity compared to TB diagnosis from pediatricians. As a conclusion, Mtb ESAT-6, CFP-10, and MPT-64 antigens can be examined in the urine of pediatric TB patients with ICT to support the diagnosis.


Antigen Mtb urine pediatric patients ICT

Article Details

How to Cite
Budiarti, A., Nugraha, J., & Wahyu W, A. D. (2018). The Examination of ESAT-6, CFP-10, MPT-64 Antigens of Mycobacterium tuberculosis in Urine of Pediatric Tuberculosis Patient with Immunochromatography to Support the Diagnosis. Folia Medica Indonesiana, 54(3), 195–199.


  1. Arora J, Kumar G, Verma AK, Bhalla M, Sarin R, Myneedu VP (2015). Utility of MPT64 antigen detection for rapid Confirmation of Mycobacterium tuberculosis Complex. J Glob Infect Dis 7, 66-69
  2. Bekmuryazeva A, Sypabekova M, Kanayeva D (2013). Tuberculosis diagnosis using immunodominant, secre-ted antigens of Mycobacterium tuberculosis. Tubercu-losis, 1-8
  3. Flynn J, Chan J, Lin P (2011). Macrophage and control of granulomatous inflammation in tuberculosis. Muco-sal Immunol 4, 271-8
  4. Ganguly N, Sharma P (2012). Mycobacterium tubercu-losis RD-1 secreted antigen as protective and risk factor for tuberculosis. Dalam buku undersstanding tuberculosis–Global experience and innovative approaches to the diagnosis rijeka. Croasia, Intech publisher
  5. Gustiani N, Parwaati I, Tjandrawati A, Lismayanti L (2014). Validitas pemeriksaan complex spesific anti-gen Mycobacterium tuberculosis region of difference 1-3 metode rapid immunocromatographypada sputum penderita tuberculosis paru. Majalah Kedokteran 46
  6. Kleinnijenhuis J, et al (2011). Innate immune recogni-tion of Mycobacterium tuberculosis. Hindawi Publish-ing Corporation Clinical and Developmental Immu-nology 2011
  7. Krishnan N, Robertson BD, Thwaites G (2010). The mechanisms and concequences of the extra-pulmonary disemination of Mycobacterium tuberculosis. Tuber-culosis (edinb) 90, 361-6
  8. Ministry of Health of Republic of Indonesia (2013). Petunjuk Teknis Manajemen TB anak, 2-79
  9. Notoatmodjo (2012). Metodologi penelitian kesehatan, Edisi Revisi. Jakarta, Rineke Xipta, p 35-43
  10. Noussair L, Bert FDR, Leflon-Guibout, Gayet N, Ni-cholas-Chanoine MH (2009). Early diagnosis of extrapulmonary tuberculosis by anew procedure com-bining broth culture and PCR. J of Clin Micro 47,1452-7
  11. Raviglione M, O’Brien R (2012). Tuberculosis. In: LoMc Graw Hill; ngo D, Kasper D, Jameson J, Fauci A, Hauser S, Loscalzo J, editor. Harrisons Principles of Internal Medicine. New York, The McGraw Hill, p 1340-1359
  12. Sari SR (2011). Nilai diagnostik antigen TB dengan Rapid Test Device (TbAg) untuk tuberkulosis paru. Unpublished thesis. Surabaya, Faculty of Medicine, Universitas Airlangga
  13. Smith I (2003). Mycobacterium tuberculosis pathogene-sis and molecular determinants of virulence. Clin Microbiol Rev 16, 463-96
  14. Thomas CE, Sexton W, Benson K, et al (2010). Urine collection and processing for protein biomarker. American Association for Cancer Research 19, 953-9
  15. Tuuminen T (2012). Urine as a specimen to diagnose infections in twenty first century: focus on analytical accuracy. Immunology 3, 1-6
  16. Wahyuningsih E (2014). Pola klinik tuberkulosis paru di RSUP Dr. Kariadi, Semarang Periode Juli 2012-Agustus 2013. Jurnal Media Medika Muda 3, 1-19
  17. Yusa, Maniam MBS (2016). Aktif dan kreatif belajar biologi. Bandung, Grafindo media pratama, p 149-153