Article Sidebar

Submitted
4 November 2016
Accepted
4 November 2016
Published
15 November 2016
Download
PDF
Statistic
Read Counter : 2068 Download : 1526

Main Article Content

Abstract

Chronic suppurative otitis media (CSOM) may cause severe morbidity and mortality and remains a major health problem worldwide. The incidence of CSOM in Indonesia (1994-1996) is estimated at about 8.36 million people and CSOM general prevalence is 3.8% (Helmi 2005). This study aims to prove the existence of bacterial biofilm in patients with safe type and hazard type CSOM from mastoidectomy. The design was observational analytic with cross sectional approach. This study was conducted at the Teratai Wards, IBP Dr. Soetomo Hospital, and Electron Microscopy Unit, Faculty of Medicine, Airlangga University Surabaya, from November 2013 to June 2014. Samples of pathological tissues were taken by consecutive sampling and bacterial biofilms examination was done by SEM. SEM results categorized the biofilm bacteria as positive or negative. Biofilm bacteria tested positive when it shows three-dimensional representation of bacteria with clear shapes and sizes and clusters, the formation of amorphous material consisting of glycocalyx material surrounding the bacteria, and surface attachment. Biofilm bacteria was regarded as negative when there is no bacterial cluster, glycocalyx and surface attachment. This study was performed on 33 CSOM patients.  Samples were divided into unsafe type CSOM (17 patients) and safe type CSOM (16 patients). Positive biofilm bacteria was found in 12 patients with unsafe type (75%) and 6 patients with safe type (35.35%). Negative biofilm bacteria was found in 4 patients with the unsafe type (25%) and 11 patients with safe type (67.9%). Logistic regression analysis revealed p value = 0.027, indicating the biofilm bacteria have a significant correlation with unsafe type CSOM (p <0.05). In conclusion, biofilm bacteria plays a role in CSOM pathogenesis of biofilm bacteria has significant correlation with unsafe type CSOM.

Keywords

chronic suppurative otitis media biofilm bacteria safe type unsafe type

Article Details

License

  • Folia Medica Indonesiana is a scientific peer-reviewed article which freely available to be accessed, downloaded, and used for research purposes. Folia Medica Indonesiana (p-ISSN: 2541-1012; e-ISSN: 2528-2018) is licensed under a Creative Commons Attribution 4.0 International License. Manuscripts submitted to Folia Medica Indonesiana are published under the terms of the Creative Commons License. The terms of the license are:

    Attribution ” You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

    NonCommercial ” You may not use the material for commercial purposes.

    ShareAlike ” If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

    No additional restrictions ” You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

    You  are free to :

    Share ” copy and redistribute the material in any medium or format.

    Adapt ” remix, transform, and build upon the material.

How to Cite
Artono, A., Purnami, N., & Rahmawati, R. (2016). BIOFILM BACTERIA PLAYS A ROLE IN CSOM PATHOGENESIS AND HAS SIGNIFICANT CORRELATION WITH UNSAFE TYPE CSOM. Folia Medica Indonesiana, 51(4), 208–213. https://doi.org/10.20473/fmi.v51i4.2846
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Akyildiz I, Take G, Uygur K, Kizil Y, Aydil U (2013). Bacterial biofilm formation in the middle-ear mucosa of chronic otitis media patients. Indian J Otolaryngol Head Neck Surg 65, 557-561
  2. Baiq MM, Ajmal M, Saeed I, Fatima S (2011). Prevalence of cholesteatoma and its complications in patients of chronic suppurative otitis media. Journal of Rawalpindi Medical College 15, 16-17
  3. Dewi NP and Zahara D (2013). Gambaran pasien otitis media supuratif kronik (omsk) di RSUP H Adam malik Medan. E-Journal FK USU 1, 1-6
  4. Drenkard E (2003). Antimicrobial resistance of Pseudomonas aeruginosa biofilms. Microbes Infect 5, 1213–1219
  5. Faridah M, Rahardjo SP, Pieter NAL (2010). Otitis media prevalence in primary school children in Makassar. The Indonesian Journal of Medical Science 1, p 385-391
  6. Hall-Stoodley L, Costerton JW, Stoodley P (2004). Bacterial biofilms: from natural environment to infectious diseases. Nat Rev Microbiol 2, 95-108
  7. Harmadji S (2004). Eradication of cholesteatoma and hearing reconstruction in malignant chronic otitis media. Folia Medica Indonesiana 40, 48-50
  8. Helmi (2005). Otitis Media Supuratif Kronis: Dalam Otitis Media Supuratif Kronis Pengetahuan Dasar Terapi Medik Mastoidektomi Timpanoplasti, Jakarta, Balai penerbit FKUI, p 55-72
  9. Kaya E, Dag I, Incesulu A, Gurbuz MK, Acar M, Birdane L (2013). Investigation of the presence of biofilms in chronic suppurative otitis media, nonsuppurative otitis media, and chronic otitis media with cholesteatoma by scanning electron microscopy. Scientific World Journal 2013, 1-6
  10. Kumar A and Prasad R (2006). Biofilms. JK Science 8, p 14-17
  11. Lampikoski H, Aarnisalo AA, Jero J, Kinnari TJ (2012). Mastoid biofilm in chronic otitis media. Otol Neurotol 33, 785-788
  12. Lee MR, Pawlowski KS, Luong A, Furze AD, Roland PS (2009). Biofilm presence in humans with chronic suppurative otitis media. Otolaryngol Head Neck Surg 141, 567-571
  13. Meyer TE, Strunk CL, Lambert PR (2006). Choleasteatoma. In: Bailey BJ, Jonhson JT, Newlands SD (eds). Head & Neck Surgery --otolaryngology, 4th ed, Philadelphia, Lippincott Williams & Wilkins, p 2082-2091
  14. Morris DP and Hagr A (2005). Biofilm: why the sudden interest. J Otolaryngol 34 Suppl 2, S56-S59
  15. Nursiah S (2003). Pola kuman aerob penyebab omsk dan kepekaan terhadap beberapa antibiotika di bagian THT FK USU/RSUP H Adam Malik Medan. Available from http://repository.usu.ac.id/bitstream/ 123456789 /6436/1/tht-siti%20 nursiah.pdf. Accessed December 13, 2013
  16. Pinar E, Oncel S, Karagoz U, Sener G, Calli C, Tatar B (2008). Demonstration of bacterial biofilms in chronic otitis media. Mediter J Otol 4, 64-68
  17. Pramesthi EDS and Ahadiah TH (2011). Evaluasi hasil operasi mastoidectomy dinding runtuh pada penderita otitis media supuratif kronik tipe bahaya di RSUD Dr. Soetomo Surabaya Januari 2007 - Desember 2008. Jurnal THT-KL 4, 23-32
  18. Saunders J, Murray M, Alleman A (2011). Biofilms in chronic suppurative otitis media and cholesteatoma: scanning electron microscopy findings. Am J Otolaryngol 32, 32-37
  19. Telian SA and Schmalbach SE (2003). Chronic otitis media. In: Snow JB and Ballenger JJ (eds). Ballenger's Otorhinolaryngology Head and Neck Surgery, 16th ed, Ontario, BC Decker Inc, p 261-265
  20. Wahyudiasih DP, Handoko E, Retnonimgsih E (2011). Hubungan jenis bakteri aerob dengan risiko tuli sensorineural penderita otitis media supuratif kronik. ORLI 41
  21. WHO (2004). Chronic suppurative otitis media: burden of illness and management options. Geneva: WHO. Available from http://www.who.int/pbd/publications/ Chronicsuppurativeotitis_media.pdf. Accessed January 7, 2013