Article Sidebar

Submitted
11 September 2023
Accepted
12 December 2023
Published
10 December 2023
Download
PDF
Statistic
Read Counter : 352 Download : 223

Main Article Content

Abstract

The excessive and uncontrolled use of pyrethroids, such as cypermethrin (CP), for pest control in Nigeria could adversely affect humans. This study aimed to investigate the oxidative stress response to cypermethrin exposure, focusing on measuring the parameters (i.e., malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)) and the potential therapeutic effects of single and co-administration of ascorbate and alpha-tocopherol. The lungs and hearts of the animals were histologically examined for cypermethrin-induced cytopathic changes. Twenty-five adult male Wistar rats weighing 180–200 g were randomly assigned to five groups, each consisting of five animals. Group I was the control group that was not subjected to any treatment. Group II was orally exposed to cypermethrin at a dosage of 10 mg/kg bw without any additional treatment. Groups III, IV, and V received cypermethrin at standard doses of 10 mg/kg bw and were orally administered with ascorbate (5,000 mg/kg bw), alpha-tocopherol (3,000 mg/kg bw), and a co-administration of ascorbate (5,000 mg/kg bw) and alpha-tocopherol (3,000 mg/kg bw), respectively. The animals were euthanized after 28 days, and samples were processed for histological analysis using hematoxylin and eosin staining. Analysis of variance (ANOVA) and Duncan's multiple range test were used to compare categorical variables of the biochemical parameters and determine the levels of MDA, SOD, GPx, and CAT. The data analysis revealed that the cypermethrin-exposed, untreated rats had elevated MDA levels and a concurrently marked decrease in SOD, GPx, and CAT activities (p<0.05). Additionally, the histopathological examination of the organs indicated inflammation and congestion. The co-administration of ascorbate and alpha-tocopherol restored the biochemical parameters more effectively compared to when the substances were administered individually. In conclusion, co-administration of ascorbic acid and alpha-tocopherol ameliorates cypermethrin-induced oxidative damage more effectively than a single administration of either substance. This may be due to the synergistic antioxidant properties of the substances.

Keywords

Cypermethrin Pollution Ascorbate Alpha-Tocopherol Toxicity

Article Details

License

Copyright (c) 2023 Folia Medica Indonesiana

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

  • Folia Medica Indonesiana is a scientific peer-reviewed article which freely available to be accessed, downloaded, and used for research purposes. Folia Medica Indonesiana (p-ISSN: 2541-1012; e-ISSN: 2528-2018) is licensed under a Creative Commons Attribution 4.0 International License. Manuscripts submitted to Folia Medica Indonesiana are published under the terms of the Creative Commons License. The terms of the license are:

    Attribution ” You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

    NonCommercial ” You may not use the material for commercial purposes.

    ShareAlike ” If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

    No additional restrictions ” You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

    You  are free to :

    Share ” copy and redistribute the material in any medium or format.

    Adapt ” remix, transform, and build upon the material.

Author Biography

Osetohanmen Flourish Ralph-Okhiria, Department of Medical Laboratory Science, University of Medical Science, Ondo, Nigeria

 

 

 

How to Cite
Temidayo Daniel Adeniyi, Akinpelu Moronkeji, & Osetohanmen Flourish Ralph-Okhiria. (2023). Ascorbate and Alpha-Tocopherol Mitigate Toxic Pathological Changes in Adult Wistar Rats Exposed to Cypermethrin. Folia Medica Indonesiana, 59(4), 391–399. https://doi.org/10.20473/fmi.v59i4.49611
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Abdus Sallam M, Zubair M, Tehseen Gul S, et al (2020). Evaluating the protective effects of vitamin E and selenium on hematology and liver, lung and uterus histopathology of rabbits with cypermethrin toxicity. Toxin Reviews, 39(3), 236–241. https://doi.org/10.1080/15569543.2018.1518335
  2. Akinpelu M, Gamade SM, Akinbo F, et al (2023). Histopathological and Biochemical Effect of Vitamin C and D on Phosphine - Induced Hepatotoxicity in Wistar Rats. Asian Journal of Dental and Health Sciences Open, 3(2), 18–22. DOI: https://doi.org/10.22270/ajdhs.v3i2.40
  3. Akorede GJ (2020). Protective e ff ect of vitamin C on chronic carbamazepine-induced reproductive toxicity in male wistar rats. Toxicology Reports, 7, 269–276. https://doi.org/10.1016/j.toxrep.2020.01.017
  4. Atere AD, Moronkeji A, Moronkeji AI, et al (2021). Serum levels of inflammatory biomarkers, glycaemic control indices and leptin receptors expression in adult male Wistar rats exposed to Pyrethroids. Journal of Cellular Biotechnology, 7(1), 41–55. https://doi.org/10.3233/JCB-210034
  5. Bhardwaj JK, Kumari P, Saraf P, et al. (2018). Antiapoptotic effects of vitamins C and E against cypermethrin-induced oxidative stress and spermatogonial germ cell apoptosis. Journal of Biochemical and Molecular Toxicology, 32(8), 1–9. https://doi.org/10.1002/jbt.22174
  6. Bouabdallah N, Mallem L, Abdennour C, et al (2022). Toxic impacts of a mixture of three pesticides on the reproduction and oxidative stress in male rats. Journal of Animal Behaviour and Biometeorology, 10 (1). https://doi.org/10.31893/JABB.22004
  7. Choudhury G, & MacNee W (2017). Role of Inflammation and Oxidative Stress in the Pathology of Ageing in COPD: Potential Therapeutic Interventions. COPD: Journal of Chronic Obstructive Pulmonary Disease, 14(1), 122–135. https://doi.org/10.1080/15412555.2016.1214948
  8. Chrustek A, HoÅ‚yÅ„ska-Iwan I, Dziembowska I et al (2018). Current research on the safety of pyrethroids used as insecticides. Medicina (Lithuania), 54(4), 1–15. https://doi.org/10.3390/medicina54040061
  9. El-Nahhal Y, & El-Nahhal I (2021). Cardiotoxicity of some pesticides and their amelioration. Environmental Science and Pollution Research, 28(33), 44726–44754. https://doi.org/10.1007/s11356-021-14999-9
  10. El Okda ES, Abdel-Hamid MAA, & Hamdy AM (2017). Immunological and genotoxic effects of occupational exposure to α-cypermethrin pesticide. International Journal of Occupational Medicine and Environmental Health, 30(4), 603–615. https://doi.org/10.13075/ijomeh.1896.00810
  11. Ensley SM (2018). Pyrethrins and Pyrethroids. In Veterinary Toxicology: Basic and Clinical Principles: Third Edition. Elsevier Inc. pp.515–520. https://doi.org/10.1016/B978-0-12-811410-0.00039-8.
  12. Ghazouani L, Feriani A, Mufti A, et al (2020). Toxic effect of alpha cypermethrin, an environmental pollutant, on myocardial tissue in male wistar rats. Environmental Science and Pollution Research, 27(6), 5709–5717. https://doi.org/10.1007/s11356-019-05336-2
  13. Grewal KK, Sandhu GS, Kaur R, et al (2010). Toxic impacts of cypermethrin on behavior and histology of certain tissues of albino rats. Toxicology International, 17(2), 94–98. https://doi.org/10.4103/0971-6580.72679
  14. Hassan SL (2019). Toxic Pathological Changes on Albino Mice after Exposures to Cypermethrin Toxic Pathological Changes on Albino Mice after Exposures to Cypermethrin. Indian Journal of Natural Sciences, 9, 16349–16354.
  15. Huang F, Chen Z, Chen H, et al (2018). Cypermethrin promotes lung cancer metastasis via modulation of macrophage polarization by targeting MicroRNA-155/Bcl6. Toxicological Sciences, 163(2), 454–465. https://doi.org/10.1093/toxsci/kfy039
  16. Bancroft JD, Suvarna KS, Layton C (2019). Bancroft's Theory and Practice of Histological Techniques. Eight edition, Elsevier Limited. https://doi.org/10.1016/s0031-3025(16)35811-1
  17. Kaur R, & Singh J (2021). Toxicity, Monitoring, and Biodegradation of Cypermethrin Insecticide: A Review. Nature Environment and Pollution Technology, 20(5), 1997–2005. https://doi.org/10.46488/NEPT.2021.V20I05.016
  18. Kaushik D, Shrma RK, & Sharma S (2018). Attenuating effects of ascorbic acid on cypermethrin induced histological and biochemical changes in developing brain of Gallus domesticus. ~ 1108 ~ Journal of Pharmacognosy and Phytochemistry, 7(6), 1108–1112. https://www.phytojournal.com/archives?year=2018&vol=7&issue=6&ArticleId=6341
  19. Oladele J, Adewale O, Oyewole O, et al (2022). Assessment of the Protective Effects of Vitamin C and E on Cypermethrin-induced Nephrotoxicity and Electrolyte Imbalance in Wistar Rats. Journal of Basic and Applied Research in Biomedicine, 6(1), 1–6. https://doi.org/10.51152/jbarbiomed.v6i1.1
  20. Oludare G, Chidinma O, Nlekerem M, et al (2017). Quail (Coturnix japonica ) egg yolk bioactive components attenuate streptozotocin-induced testicular damage and oxidative stress in diabetic rats. European Journal of Nutrition, 57(8), 2857–2867. https://doi.org/10.1007/s00394-017-1554-4
  21. Pisoschi AM, & Pop A. (2015). The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry, 97, 55–74. https://doi.org/10.1016/j.ejmech.2015.04.040
  22. Wang J, & Dong W (2018). Oxidative stress and bronchopulmonary dysplasia. Gene, 678, 177–183. https://doi.org/10.1016/j.gene.2018.08.031
  23. Ye M, Beach J, Martin JW et al (2017). Pesticide exposures and respiratory health in general populations. Journal of Environmental Sciences 51, 361–370. https://doi.org/10.1016/j.jes.2016.11.012