Main Article Content


The aim of this study was to prove the effects of dexamethasone on immune responses in rats with testicular torsion. This was an experimental study with post test only control group design. In this study, 27 rats were randomly divided into 3 groups. In normal or sham group (CN) orchidectomy was performed on the right testis on day 14. The positive control group (CP) had testicular torsion for 10 h, then manual detorsion was performed after 4 h, and on day 14 orchidectomy was performed on ipsilateral and contralateral testes. The treatment group (N) had testicular torsion for 10 h and received 10 mg/kgBW dexamethasone subcutaneously 30 minutes before manual detorsion 4 hours later, and on day 14 orchidectomy was performed to ipsilateral and contralateral testes. The spermatogonium count was calculated based on the average number of intratubular seminiferous tubule section observed in contralateral testicular incision with Haematoxiliyn Eosin (HE) staining. IgG count was calculated based on the average number of extratubular seminiferous tubules observed at 5 contralateral testicular incision with immunohistochemical staining (IHC). The number of spermatogonia was significantly different between CN and CP, CN and N, and CP and N (p<0.05). IgG production also showed significant differences between CN and CP, CN and N, as well as CP and N (p<0.05). In conclusion, administration of dexamethasone provides a protective effect on spermatogonium count and IgG production on a 10-hour testicular torsion.


Dexamethasone testicular torsion spermatogonium IgG

Article Details

How to Cite
Kustono, A., Sudiana, I. K., Rizaldi, F., & Tarmono, T. (2018). Effects of Dexamethasone on Contralateral Testicular Immune Response in Wistar Strain Rats with Unilateral Testicular Torsion. Folia Medica Indonesiana, 54(1), 29–33.


  1. Barker K, Raper FP (1964). Torsion of the testis. Br. Journal of Urology 36, 35-41
  2. Doerge RF (1982). Ed. Wilson and Gisvol’s textbook of organic and pharmaceutical chemistry. 8th ed. Phila-delphia, J.B. Lippincott
  3. Hardjowijoto S (2004). Pengaruh torsio testis unilateral terhadap modulasi respon imun dan peningkatan apoptosis pad testis kontralateral. A dissertation. Surabaya, Universitas Airlangga
  4. Cuckow PM, Frank JD (2000). Version of Record online. BJU International 86, 349-353
  5. Purnomo, BP (2003). Dasar-dasar urologi. Jakarta, Sagung Seto, p 145-148
  6. Saez JM, Morera AM, Haour F, Evain D (1977). Effects of in vivo administration of dexamethasone, corticotropin and human chorionic gonadotropin on steroidogenesis and protein and DNA synthesis of testicular interstitial cells in prepuberal rats. Endocrinology 101, 1256-1263
  7. Sjamsuhidajat R, De Jong W (2004). Buku ajar ilmu bedah. 2nd ed. Jakarta, Penerbit Buku Kedokteran – EGC, p 799
  8. Subowo (1993). Immuno biologi. Bandung, Angkasa, p 17-20, 53-57
  9. Turek PJ (1997). Immunopathology and infertility. In infertility in the male. Eds. Lipshultz LI and Howard SS. 3rd ed. Missouri, St Loui, p 305-325