Purple Rosella (Hibiscus sabdariffa Linn.) Petal Extract Prevents Hepatocyte Degeneration in Wistar Rats Exposed to Cigarette Smoke

Arya Ulilalbab, Bambang Wiratmadi, Merryana Adriani

= http://dx.doi.org/10.20473/fmi.v54i2.8857
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Abstract


Currently, the number of smokers in Indonesia is still high. Cigarette smoke contains free radicals. This study aimed to analyze the effects of purple rosella petal extract on the prevention of hepatocyte degeneration in Wistar rats exposed to cigarette smoke. Twenty male rats were divided into four groups: negative control, positive control, treatment group receiving 270 mg/kg BW purple rosella petal extract, and treatment group receiving 540 mg/kg BW purple rosella calyx extract. Negative control was only given with standard feed. Positive control was given with standard feed and exposed to 2 cigarettes a day. Treatment groups were fed with standard feed and administered with rosella extract in the morning then exposed to 2 cigarettes every day. This study was conducted for 28 days. At the end of the study, hepatocyte degeneration was observed in liver histopathology stained with hematoxilin eosin. The administration of purple rosella petal extracts in the doses of 540 mg/kgBW and 270 mg/kgBW significantly (p<0.05) prevented hepatocyte degeneration. Purple rosella petal extract is able to prevent hepatocyte degeneration in Wistar rats exposed to cigarette smoke.

Keywords


Antioxidant; free radical; hepatocyte degeneration; purple rosella

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References


Adetutu A, Owoade AO (2013). Hepatoprotective and antioxidant effect of hibiscus polyphenol rich extract (Hpe) against carbon tetrachloride (CCl4) – Induced damage in rats. British Journal of Medicine and Medical Research 3, 1574-1586

Adeyemi DO, Ukwenya VO, Obuotor EM, et al (2014). Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage. Bmc Complementary and Alternative Medi-cine 14, 277

Ajiboye TO, Salawu NA, Yakubu MT, et al (2011). Antioxidant and drug detoxification potentials of Hibiscus sabdariffa anthocyanin extract. Drug and Chemical Toxicology 34, 109-15

Alarco-Alanso J, Zamilpa A, Aguilar FA, et al (2012). Pharmacological characterization of the diuretic effect of Hibiscus sabdariffa Linn (Malvaceae) extract. Journal of Ethnopharmacology 139, 751-6

Ambrose JA, Barua RS (2004). The pathophysiology of cigarette smoking and cardiovascular disease: An update. Journal of the American College of Cardiology 43, 1731-1737

Bolade O, Ojo O (2009). Comercial practice of rosella (Hibiscus sabdariffa L), beverage production. Word Journal of Agricultural Science 5, 126-131

Cross CE, Traber M, Eiserich J, et al (1999). Micronutrient antioxidants and smoking. British Medical Bulletin 55, 691-704

Esa NM, Hern FS, Ismail A, et al (2010). Antioxidant activity in different parts of rosella (Hibiscus sabdariffa L.) extracts and potential exploitation of the seeds. Food Chemistry 122, 1055-1060

Famurewa AC, Kanu SC, Uzoegwu PN, et al (2015). Ameliorative effects of Hibiscus sabdariffa extract against carbon tetrachloride-induced lipid peroxi-dation, oxidative stress and hepatic damage in rats. Journal of Pharmaceutical and Biomedical Sciences 05, 725-732

Frei B, Forte TM, Ames, BN, et al (1991). Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoprotein properties in human blood plasma. Biochemical Journal 277, 133-138

Guyton, Hall (1997). Buku ajar fisiologi kedokteran. 9th Ed. Jakarta, Penerbit Buku Kedokteran EGC

Hirunpanich V, Utaipat A, Morales NP, et al (2005). Antioxidant effect of aqueous extracts from dried calyx of Hibiscus sabdariffa L. in vitro using rat low-density lipoprotein. Biology Pharmacy 28, 481-484

Jain A, Agrawal BK, Varma M, et al (2009). Anti-oxidant status and smoking habits: Relationship with diet. Singapore Medical Journal. 50, 624-627

Jawi IM, Manuaba IBR, Sutirtayasa IWP, et al (2006). Pemberian glutamin menurunkan kadar bilirubin darah serta mengurangi nekrosis sel-sel hati setelah pemberian aktivitas fisik maksimal dan parasetamol pada mencit. Dexa Medica 4

Kementerian Kesehatan Republik Indonesia (2015). Perilaku merokok masyarakat Indonesia: Berdasarkan RISKESDAS 2007 dan 2013. Jakarta, Pusat Data dan Informasi Kementerian Kesehatan RI, p.1-12

Kurnia H, Permatasari N, Subandi (2011). Pengaruh ekstrak jintan hitam terhadap MDA dan sel sperma-togonium tikus yang dipapar asap rokok kretek subakut. Jurnal Kedokteran Brawijaya 26, 161-165

Marwan, Widjajanto E, Karyono S (2005). Pengaruh pemberian ekstrak biji jinten hitam (Nigella sativa) terhadap kadar GSH, MDA, jumlah serta fungsi sel makrofag alveolar paru tikus wistar yang dipapar asap rokok kronis. Jurnal Kedokteran Brawijaya 21(3): 111-121

Maulana HI, Ulilalbab A, Priyanto AD, et al (2014). Effervescent rosella ungu mencegah penurunan nilai SOD dan mencegah nekrosis hepar tikus wistar yang diberi minyak jelantah. Jurnal Kedokteran Brawijaya 28, 85-90

Muliartha IKG, Sriwahyuni E, Yuliawati (2009). Pemberian kombinasi vitamin C dan E peroral memperbaiki kerusakan hepar akibat paparan rokok kretek sub kronik. Jurnal Kedokteran Brawijaya 24, 23-27

Nasution AS, Wirjadmadi B, Adriani M (2016). Efek preventif pemberian ekstrak kulit buah naga berdaging super merah (Hylocereus costaricensis) terhadap malondialdeid tikus wistar yang dipapar asap rokok. Jurnal Kedokteran Brawijaya 29, 21-24

Park EM, Park YM, Gwak YS (1998). Oxidative damaged tissue of rats exposed to cigarette smoke. Free Radicals Biology Medicine 25, 79-86

Prakash (2001). Antioxidant activity. Medallion Labora-tories Analitical Progress 19, 1-4

Simanjuntak D, Sudaryati E (1998). Aspek pencegahan radikal bebas melalui antioksidan. Majalah Kedokter-an Indonesia 48, 50-4

Soini P, Lehto VP (1998). Histopathological evaluation of apoptosis in cancer. The American Journal of Pathology 153, 1041-1053

Subandi (1999). Efek antioksidan (vit. C) terhadap jumlah fungsi makrofag alveoli serta kadar SOD jaringan paru tikus yang dipapar dengan asap rokok kronis. A dissertation. Malang, Universitas Brawijaya

Tirtosastro S, Murdiyati AS (2010). Kandungan kimia tembakau dan rokok. Buletin Tanaman Tembakau, Serat dan Minyak Industri 2, 33-43

Ulilalbab A, Priyanto AD, Maulana HI, et al (2012). Pemberian tablet effervescent rosella ungu menurun-kan nilai MDA (malondialdehid) tikus wistar yang dipapar minyak jelantah. The Indonesian Journal of Public Health 9, 81-86

Usoh IF, Ekaidem IS, Etim OE, et al (2012). Antioxidant and hepatoprotective effects of dried flower extracts of Hibiscus sabdariffa L. on rats treated with carbon tetrachloride. Journal of Applied Pharmaceutical Science 02, 186-189

Valvanidis A, Haralambous E (2001). A comparative study by electron paramagnetic resonance of free radicals species in the mainsteam and sidestream smoke of cigarettes with conventional aceate filters and “bio-filters”. Redox Report 6, 161-171

Voges E (2000). Tobacco encyclopedia. Tabac Journal International, Mainz, Germany 279p.

Vollmar B, Menger MD (2009). The hepatic micro-circulation: mechanistic contributions and therapeutic targets in liver injury and repair. Physiological Reviews 89, 1269-1339

Yusmiati SNH, Arbai AMB, Tjokroprawiro A, et al (2012). Potensi antioksidan dalam ekstrak teh merah (Hibiscus sabdariffa) dan teh hijau (Camellia sinensis) terhadap proses aterogenesis pada tikus dengan diet aterogenik. JBP 14, 158-171


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