The Role of Mycobacterium Tuberculosis Detection on Oral Mucosa in Diagnosis of Pulmonary Tuberculosis
Downloads
Background. Tuberculosis is one of the world's health problems, with larger mortality rate than HIV/AIDS. In order to control this disease, a new and inexpensive diagnostic method is needed. Oral samples, such as buccal mucosa epithelium, can be an alternative specimen, other than sputum, in the diagnosis of tuberculosis. Purpose. This study aimed to analyze the presence of Mycobacterium tuberculosis on buccal swabbing of tuberculosis patients. Method. Study was conducted on 18 new pulmonary tuberculosis patients in TB DOTS unit of Dr. Soetomo General Hospital. Each subject was swabbed on the buccal mucosa of the oral cavity for subsequent examination of PCR, AFB, and culture to detect Mycobacterium tuberculosis in the oral cavity. Result. The results showed that Mycobacterium tuberculosis was detected from 2 study subjects (11%) using PCR, whereas in AFB and culture examination methods no Mycobacterium tuberculosis was detected on buccal mucosa of the oral cavity. Conclusion. This study showed that Mycobacterium tuberculosis was detected on buccal mucosa swab of tuberculosis patients based on PCR technique, but this method is less suitable in diagnosis of pulmonary tuberculosis.
Organization WH. Global Tuberculosis Report 2022. Geneva; 2022.
Perhimpunan Dokter Paru Indonesia. Tuberkulosis Pedoman Diagnosis Dan Penatalaksanaan Di Indonesia. Vol 001.; 2021.
Yang F, Zeng X, Ning K, et al. Saliva microbiomes distinguish caries-active from healthy human populations. ISME J. 2012;6:1-10. doi:10.1038/ismej.2011.71
Leung E, Minion J, Benedetti A, Pai M, Menzies D. Microcolony culture techniques for tuberculosis diagnosis: a systematic review. Int J Tuberc Lung Dis. 2012;16(1):16-23. doi:10.5588/ijtld.10.0065
Chang K, Lu W, Wang J, et al. Rapid and effective diagnosis of tuberculosis and rifampicin resistance with Xpert MTB / RIF assay : A meta-analysis. J Infect. 2012;(March):1-9. doi:10.1016/j.jinf.2012.02.012
Luabeya AK, Wood RC, Shenje J, et al. Noninvasive detection of tuberculosis by oral swab analysis. J Clin Microbiol. 2019;57(3). doi:10.1128/JCM.01847-18
Mesman AW, Calderon R, Soto M, et al. Mycobacterium tuberculosis detection from oral swabs with Xpert MTB/RIF ULTRA: A pilot study. BMC Res Notes. 2019;12(1):10-12. doi:10.1186/s13104-019-4385-y
Bayraktar K, Gürer G. Pulmonary tuberculosis presenting with oral aphthae. Eur J Rheumathology. 2015:1-3. doi:10.5152/eurjrheum.2015.0098
Stinson KW, Eisenach K, Kayes S, et al. Mycobacteriology Laboratory Manual Editor ,1st Ed. japan: Global Laboratory Initiative; 2014.
Kwiecien R, Kopp-schneider A, Blettner M. Concordance Analysis. Dtsch Arztebl Int. 2011;108(30):515-521. doi:10.3238/arztebl.2011.0515
Kim SY, Byun JS, Choi JK, Jung JK. A case report of a tongue ulcer presented as the first sign of occult tuberculosis. BMC Oral Health. 2019;19(1):1-5. doi:10.1186/s12903-019-0764-y
Krawiecka E, Szponar E. Tuberculosis of the oral cavity: an uncommon but still a live issue. Adv Dermatology Allergol. 2015;32(4):302-306. doi:10.5114/pdia.2014.43284
Mishra AK, Kant S, Verma L, et al. Coexistence of carcinoma cheek with tuberculosis : a rarity. Int J Res Med Sci. 2015;3(3):780-782. doi:10.5455/2320-6012.ijrms20150349
Porteous NB, Terézhalmy GT. Exposure Control Issues for Oral Healthcare Workers. Contin Educ Course. 2013:1-24.
Jain P, Jain I. Oral manifestations of tuberculosis: Step towards early diagnosis. J Clin Diagnostic Res. 2014;8(12):ZE18-ZE21. doi:10.7860/JCDR/2014/10080.5281
Eguchi J, Ishihara K, Watanabe A, Fukumoto Y, Okuda K. PCR method is essential for detecting Mycobacterium tuberculosis in oral cavity samples. Oral Microbiol Immunol. 2003;18(25):156-159.
Davis JL, Huang L, Kovacs JA, et al. Polymerase Chain Reaction of secA1 on Sputum or Oral Wash Samples for the Diagnosis of Pulmonary Tuberculosis. Clin Infect Dis. 2009;94110(48):725-732. doi:10.1086/597038
Palakuru SK, Lakshman VK, Bhat KG. Microbiological analysis of oral samples for detection of Mycobacterium tuberculosis by nested polymerase chain reaction in tuberculosis patients with periodontitis. Dent Res J (Isfahan). 2012;9(6):688-693.
Wood RC, Luabeya AK, Weigel KM, et al. Detection of Mycobacterium tuberculosis DNA on the oral mucosa of tuberculosis patients. Sci Rep. 2015:1-5. doi:10.1038/srep08668
Shenai S, Amisano D, Ronacher K, et al. Exploring Alternative Biomaterials for Diagnosis of Pulmonary Tuberculosis in HIV-Negative Patients by Use of the GeneXpert MTB / RIF Assay. J Clin Microbiol. 2013;51(12):4161-4166. doi:10.1128/JCM.01743-13
Gill JS, Sandhu S, Gill S. Primary tuberculosis masquerading as gingival enlargement. Nat Publ Gr. 2010;208(8):343-345. doi:10.1038/sj.bdj.2010.344
Yang S, Zhan P, Mao H, Shi X, Wang L. Perfusing chemotherapy by percutaneous lung puncture " holing ” for pulmonary tuberculoma ” a ten-year single center experience. J Thorac Dis. 2009;5(4):2013. doi:10.3978/j.issn.2072-1439.2013.07.03
Crevel R van, Koesoemadinata R, Hill PC, Harries AD. Clinical management of combined tuberculosis and diabetes. Int J Tuberc Lung Dis. 2018;22(12):1404-1410.
Caulfield AJ, Wengenack NL. Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Diagnosis of active tuberculosis disease : From microscopy to molecular techniques. J Clin Tuberc Other Mycobact Dis. 2016;4:33-43. doi:10.1016/j.jctube.2016.05.005
Copyright (c) 2023 Reiska Kumala Bakti, Bagus Soebadi, Diah Savitri Ernawati, Ni Putu Made Mertaniasih
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access journal, and articles are distributed under the terms of the Creative Commons Lisence, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Copyright notice:
IJDM by UNAIR is licensed under a Creative Commons Atribusi 4.0 Internasional.
- The journal allows the author to hold the copyright of the article without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions.
- The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution (CC BY)
