First Report on Molecular Identification of Caulerpa Green Algae from Mandangin Island Indonesia Using Partial 18SrRNA Genes
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Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandangin Island. This study aimed to identify Caulerpa sp. based on molecular analysis using certain genetic markers. This research is expected to provide information on the identification of macroalgae from Indonesia waters, especially Mandangin Island, Madura with the use of molecular analysis based on 18SrRNA primers. The two green seaweed samples from the Caulerpa genus in this study were successfully analyzed using 18SrRNA primers. The BLAST results of samples 1and 2 are related to Caulerpa taxifolia 18SrRNA, but in the phylogenetic tree result, Sample 1 was more closely related to Caulerpa sertularioides f. longipes. 18SrRNA primers have been used for molecular identification of green seaweed from Mandangin for the first time and this shows that barcode markers can be used for molecular identification of seaweed, specifically Caulerpa in the waters of Mandangin Island, Indonesia.
Balata, D., Piazzi, L. and Cinelli, F., 2004. A comparison among assemblages in areas invaded by Caulerpa taxifolia and C. racemosa on a subtidal Mediterranean rocky bottom. Marine Ecology, 25(1), pp.1-13. https://doi.org/10.1111/j.1439-04 85.2004.00013.x.
Belton, G.S., van Reine, W.F.P.H., Huisman, J.M., Draisma, S.G. and D. Gurgel, C.F., 2014. Resolving phenotypic plasticity and species designation in the morphologically challenging Caulerpa racemosa–peltata complex (Chlorophyta, Caulerpaceae). Journal of Phycology, 50(1), pp.32-54. https://doi.org/10 .1111/jpy.12132.
Bulleri, F., Balata, D., Bertocci, I., Tamburello, L. and Benedetti-Cecchi, L., 2010. The seaweed Caulerpa racemosa on Mediterranean rocky reefs: from passenger to driver of ecological change. Ecology, 91(8), pp.2205-2212. https://doi.org/10.1890/09-1857.1.
Camacho, O., Mattio, L., Draisma, S., Fredericq, S. and Diaz-Pulido, G., 2015. Morphological and molecular assessment of Sargassum (Fucales, Phaeophyceae) from Caribbean Colombia, including the proposal of Sargassum giganteum sp. nov., Sargassum schnetteri comb. nov. and Sargassum section Cladophyllum sect. nov. Systematics and Biodiversity, 13(2), pp.105-130. https://doi.org/10.1080/14772000.2014.972478.
Chisholm, J.R., Fernex, F.E., Mathieu, D. and Jaubert, J.M., 1997. Wastewater discharge, seagrass decline and algal proliferation on the Cote d'Azur. Marine Pollution Bulletin, 34(2), pp.78-84. https:// doi.org/10.1016/S0025-326X(96)0 0072-0.
Cho, M., Lee, H.S., Kang, I.J., Won, M.H. and You, S., 2011. Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. Food Chemistry, 127(3), pp.999-1006. https://doi. org/10.1016/j.foodchem.2011.01.072.
Chomczynski, P., 1993. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques, 15(3), pp.532-4. https://europepmc .org/article/med/7692896
Dawson, E.Y., 1954. Marine plants in the vicinity of the Institut Océanographique de Nha Trang, Viíªt Nam. Pacific Science, 8, pp.373-469. https://www.semanticscholar. org/paper/Marine-Plants-in-the-Vicinity-of-the-Institut-de-Dawson/ b8f4d6a47163baaed213d37efb42e1eea47ba7ce.
Dharmayanti, N., Abinawanto and Anti, A., 2018, May. Using internal transcribed spacers 2 (ITS2) to identify seaweed species from Tomini Bay and Banten Bay. In IOP Conference Series: Earth and Environmental Science (Vol. 278, No. 1, p.012017). IOP Publishing. https: //doi.org/10.1088/1755-1315/278 /1/012017.
Doyle, J.J. and Doyle, J.L., 1990. Isolation ofplant DNA from fresh tissue. Focus, 12(13), pp.39-40. https:// www.researchgate.net/profile/Adhityo_Wicaksono/post/Quick_Extraction_of_plant_DNA_does_anyone_have_protocol/attachment/59d642dcc49f478072eaba57/AS:273804717166592@1442291539148/download/Doyle&Doyle_Focus_1990_CTAB.pdf
Farasat, M., Khavari-Nejad, R.A., Nabavi, S.M.B. and Namjooyan, F., 2013. Antioxidant properties of two edible green seaweeds from northern coasts of the Persian Gulf. Jundishapur journal of natural pharmaceutical products, 8(1), p.47 –52. https://doi.org/10.17795/jjnp p-7736.
Gennaro, P. and Piazzi, L., 2011. Synergism between two anthropic impacts: Caulerpa racemosa var. cylindracea invasion and seawater nutrient enrichment. Marine Ecology Progress Series, 427, pp.59-70. http:/ /dx.doi.org/10.3354/meps09053.
Guiry, M.D., Guiry, G.M., Morrison, L., Rindi, F., Miranda, S.V., Mathieson, A.C., Parker, B.C., Langangen, A., John, D.M., Bárbara, I. and Carter, C.F., 2014. AlgaeBase: an on-line resource for algae. Cryptogamie, Algologie, 35(2), pp.105-115. https: //doi.org/10.7872/crya.v35.iss2.2014.105.
Hoarau, G., Coyer, J.A., Stam, W.T. and Olsen, J.L., 2007. A fast and inexpensive DNA extraction/ purification protocol for brown macroalgae. Molecular Ecology Notes, 7(2), pp.191-193. https:// doi.org/10.1111/j.1471-8286.2006. 01587.x.
Jousson, O., Pawlowski, J., Zaninetti, L., Meinesz, A. and Boudouresque, C.F., 1998. Molecular evidence for the aquarium origin of the green alga Caulerpa taxifolia introduced to the Mediterranean Sea. Marine Ecology Progress Series, 172, pp.275-280. https://doi.org/10.3354/meps172275.
Kanegawa, K., Harada, H., Myouga, H., Katakura, Y., Shirahata, S. and Kamei, Y., 2000. Telomerase inhibiting activity in vitro from natural resources, marine algae extracts. Cytotechnology, 33(1-3), pp.221-227. https://dx.doi.org/10. 1023%2FA%3A1008179302906.
Kher, C.P., Doerder, F.P., Cooper, J., Ikonomi, P., Achilles-Day, U., Küpper, F.C. and Lynn, D.H., 2011. Barcoding Tetrahymena: discriminating species and identifying unknowns using the cytochrome c oxidase subunit I (cox-1) barcode. Protist, 162(1), pp.2-13. https://doi.org/10.1016/j.protis.2010.03.004.
Lane, C.E., Lindstrom, S.C. and Saunders, G.W., 2007. A molecular assessment of northeast Pacific Alaria species (Laminariales, Phaeophyceae) with reference to the utility of DNA barcoding. Molecular phylogenetics and evolution, 44(2), pp.634-648. https://doi.org/10.1016/j.ympev.2007.03.016.
Lantah, P.L., Montolalu, L.A.D.Y. and Reo, A.R., 2017. Kandungan fitokimia dan aktivitas antioksidan ekstrak metanol rumput laut Kappaphycus alvarezii. Media Teknologi Hasil Perikanan, 5(3), pp.73-79. https:// doi.org/10.35800/mthp.5.3.2017.16785.
Le Gall, L. and Saunders, G.W., 2010. Dna barcoding is a powerful tool to uncover algal diversity: A case study of the Phyllophoraceae (Gigartinales, Rhodophyta) in the Canadian flora 1. Journal of phycology, 46(2), pp.374-389. https: //doi.org/10.1111/j.1529-8817.20 10.00807.x.
Liu, Y., Morgan, J.B., Coothankandaswamy, V., Liu, R., Jekabsons, M.B., Mahdi, F., Nagle, D.G. and Zhou, Y.D., 2009. The Caulerpa pigment caulerpin inhibits HIF-1 activation and mitochondrial respiration. Journal of natural products, 72(12), pp.2104-2109. https://doi.org/10.1021/np9005794.
Mahendran, S. and Saravanan, S., 2017. Molecular taxonomy of green seaweeds Ulva lactuca and Caulerpa taxifolia through phylogenetic analysis. Indian Journal of Geo Marine Sciences. 46(2), pp.414-419. http://nopr.niscair.res.in/handle/123456789/40765
Matanjun, P., Mohamed, S., Mustapha, N.M., Muhammad, K. and Ming, C.H., 2008. Antioxidant activities and phenolics content of eight species of seaweeds from north Borneo. Journal of Applied Phycology, 20(4), p.367-373. https:/ /doi.org/10.1007/s10811-007-926 4-6.
Mattio, L. and Payri, C., 2010. Assessment of five markers as potential barcodes for identifying Sargassum subgenus Sargassum species (Phaeophyceae, Fucales). Cryptogamie Algologie, 31(4), p.467-485. http://science press.mnhn.fr/sites/default/files/articles/pdf/cryptogamie-algologie20 10v31f4a21.pdf
McKinnon, J.G., Gribben, P.E., Davis, A.R., Jolley, D.F. and Wright, J.T., 2009. Differences in soft-sediment macrobenthic assemblages invaded by Caulerpa taxifolia compared to uninvaded habitats. Marine ecology progress series, 380, pp.59-71. http:/ /dx.doi.org/10.3354/meps07926.
Meinesz, A., De Vaugelas, J., Hesse, B. and Mari, X., 1993. Spread of the introduced tropical green alga Caulerpa taxifolia in northern Mediterranean waters. Journal of applied Phycology, 5(2), pp.141-147. https://doi.org/10.1007/BF00004009.
Oakes, J.M., Bautista, M.D., Maher, D., Jones, W.B. and Eyre, B.D., 2011. Carbon self"utilization may assist Caulerpa taxifolia invasion. Limnology and oceanography, 56(5), pp.1824-1831. https://doi.org/10.4 319/lo.2011.56.5.1824.
Pacciardi, L., De Biasi, A.M. and Piazzi, L., 2011. Effects of Caulerpa racemosa invasion on soft-bottom assemblages in the Western Mediterranean Sea. Biological invasions, 13(12), pp.2677-2690. https://doi.org/10.1007/s10530-01 1-9938-5.
Piazzi, L., Ceccherelli, G. and Cinelli, F., 2001. Threat to macroalgal diversity: effects of the introduced green alga Caulerpa racemosa in the Mediterranean. Marine ecology progress series, 210, pp.149-159. https://doi.org/10.3354/meps210149.
Poong, S.W., Lim, P.E., Phang, S.M., Sunarpi, H., West, J.A. and Kawai, H., 2014. A molecular-assisted floristic survey of crustose brown algae (Phaeophyceae) from Malaysia and Lombok Island, Indonesia based on rbcL and partial cox1 genes. Journal of applied phycology, 26(2), pp.1231-1242. https://doi.org/10.1007/s10811-01 3-0081-9.
Santiago-Vázquez, L.Z., Ranzer, L.K. and Kerr, R.G., 2006. Comparison of two total RNA extraction protocols using the marine gorgonian coral Pseudopterogorgia elisabethae and its symbiont Symbiodinium sp. Electronic Journal of Biotechnology, 9(5), pp. 599-603. https://doi.org/ 10.2225/vol9-issue5-fulltext-15.
Saunders, G.W. and Moore, T.E., 2013. Refinements for the amplification and sequencing of red algal DNA barcode and RedToL phylogenetic markers: a summary of current primers, profiles and strategies. Algae, 28(1), pp.31-43. https://doi. org/10.4490/algae.2013.28.1.031.
Setyawan, A., 2019. Fucoidan dari Alga Cokelat Tropis Sebagai Imunostimulan Udang Vannamei (Litopenaeus vannamei): Kajian Hematologi, Ekspresi Gen-gen Imun, Resistensi Terhadap WSSV dan Pertumbuhan. Disertasi. Universitas Gadjah Mada.Yogyakarta.
Shanmugam, H., Sathasivam, R., Rathinam, R., Arunkumar, K. and Carvalho, I.S., 2018. Algal Biotechnology: An Update From Industrial and Medical Point of View. In Omics Technologies and Bio-Engineering, pp. 31-52. Academic Press. https://doi.org/10.1016/b97 8-0-12-815870-8.00003-6.
Soylu, E.N. and Gönülol, A., 2012. Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. Turkish Journal of Biology, 36(3), pp.247-254. https://doi.org/ 10.3906/biy-1001-19.
Stancheva, R., Hall, J.D., McCourt, R.M. and Sheath, R.G., 2013. Identity and phylogenetic placement of Spirogyra species (Zygnematophyceae, Charophyta) from California streams and elsewhere. Journal of phycology, 49(3), pp.588-607. https ://doi.org/10.1111/jpy.12070.
Tan, J., Lim, P.E., Phang, S.M., Hong, D.D., Sunarpi, H. and Hurtado, A.Q., 2012. Assessment of four molecular markers as potential DNA barcodes for red algae Kappaphycus Doty and Eucheuma J. Agardh (Solieriaceae, Rhodophyta). PLoS one, 7(12), pp.1-15. https://doi.org/10.1371/ journal.pone.0052905.
Varela"ílvarez, E., Andreakis, N., Lago"Lestón, A., Pearson, G.A., Serrao, E.A., Procaccini, G., Duarte, C.M. and Marba, N., 2006. Genomic DNA isolation from green and brown algae (caulerpales and fucales) for microsatellite library construction. Journal of phycology, 42(3), pp.741-745. https://doi.org/10.1111/j.152 9-8817.2006.00218.x.
Vázquez-Luis, M., Sanchez-Jerez, P. and Bayle-Sempere, J.T., 2010. Effects of Caulerpa racemosa var. cylindracea on prey availability: an experimental approach to predation of amphipods by Thalassoma pavo (Labridae). Hydrobiologia, 654(1), pp.147-154. https://doi.org/10.1007/s10750-01 0-0378-5.
Verlaque, M. and Fritayre, P., 1994. Mediterranean algal communities are changing in the face of the invasive Alga Caulerpa-Taxifolia (Vahl) C Agardh. Oceanologica acta, 17(6), pp.659-672.
Vinayak, R.C., Sudha, S.A. and Chatterji, A., 2011. Bio"screening of a few green seaweeds from India for their cytotoxic and antioxidant potential. Journal of the Science of Food and Agriculture, 91(13), pp.2471-2476. https://doi.org/10.1002/jsfa.4490.
Wang, R., Liu, H., Wang, Y., Ke, H., Fan, J., Chen, F. and Tan, W., 2019. Complete chloroplast genome of green alga Caulerpa sertularioides f. longipes (J. Agardh) Collins, 1909. Mitochondrial DNA Part B, 4(2), pp.3573-3574. https://doi.org/10.1 080/23802359.2019.1676668
Wongsawad, P. and Peerapornpisal, Y., 2014. Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers. Saudi journal of biological sciences, 21(5), pp.505-510. https:// doi.org/10.1016/j.sjbs.2014.01.003.
Yeh, W.J. and Chen, G.Y., 2004. Nuclear rDNA and internal transcribed spacer sequences clarify Caulerpa racemosa vars. from other Caulerpa species. Aquatic botany, 80(3), pp.193-207. https://doi.org/10.101 6/j.aquabot.2004.07.008.
Zubia, M., Robledo, D. and Freile-Pelegrin, Y., 2007. Antioxidant activities in tropical marine macroalgae from the Yucatan Peninsula, Mexico. Journal of applied phycology, 19(5), pp.449-458. https://doi.org/ 10.1007/s108 11-006-9152-5.
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