Viability and Antibacterial Activity of Bifidobacterium bifidum in Fermented Robusta Coffee for Diarrhea Treatment
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Background: Diarrhea can be treated with probiotic bacteria such as Bifidobacterium bifidum, which decreases the intestinal environment's pH to become acidic so that pathogenic bacteria cannot thrive. Objective: To make fermented coffee that can increase the number of probiotic bacteria Bifidobacterium bifidum and has antidiarrheal activity against pathogenic bacteria Escherichia coli. Methods: Robusta coffee (20.25% and 19.75%) was fermented using Saccharomyces cerevisiae, and then the probiotic bacteria Bifidobacterium bifidum was added. Unfermented coffee was compared with the same concentration (20.25% and 19.75%) to obtain four formulas. Organoleptic panelists tested all formulas to determine the best formula for fermented and non-fermented coffee. The number of Bifidobacterium bifidum and antibacterial activity was calculated on the optimum formulation using the Total Plate Count and Disc Diffusion Method. Result: The optimum formula obtained at fermented and unfermented coffee concentration was 20,25%. The number of probiotic bacteria Bifidobacterium bifidum growing in fermented and non-fermented coffee was 7.3 x 108 ± 32.4 and 3.1 x 108 ± 30.7 (p < 0.05). The diameter of the inhibition zone of the best fermented and non-fermented coffee was 11.5 ± 0.5 mm and 8.5 ± 0.5 mm, respectively (p < 0.05). Conclusion: Fermented coffee can increase the growth of the probiotic bacteria Bifidobacterium bifidum and has strong antibacterial activity against Escherichia coli bacteria.
Abdelhamid, A. G., Esaam, A. & Hazaa, M. M. (2018). Cell Free Preparations of Probiotics Exerted Antibacterial and antibiofilm Activities Against Multidrug Resistant E. coli. Saudi Pharmaceutical Journal; 26; 603–607. doi: 10.1016/j.jsps.2018.03.004.
Amine, K. M., Champagne, C. P., Salmieri, S., Britten, M., St-Gelais, D., Fustier, P. & Lacroix, M. (2014). Effect of Palmitoylated Alginate Microencapsulation on Viability of Bifidobacterium longum during Freeze-drying. Food Science and Technology; 56; 111-117. doi: 10.1016/j.lwt.2013.11.003.
Arslanoglu, S., Moro, G. E., Schmitt, J., Tandoi, L., Rizzardi, S. & Boehm, G. (2008). Early Dietary Intervention with a Mixture of Prebiotic Oligosaccharides Reduces the Incidence of Allergic Manifestations and Infections during the First Two Years of Life. Journal of Nutrition; 138; 1091–1095. doi: 10.1093/jn/138.6.1091.
Bermudez-Brito, M., Plaza-Diaz, J., Muñoz-Quezada, S., Gómez-Llorente, C. & Gil, A. (2012). Probiotic Mechanisms of Action. Annals of Nutrition and Metabolism; 61; 160–174.
Bondue, P. & Delcenserie, V. (2015). Genome of Bifidobacteria and Carbohydrate Metabolism. Korean Journal for Food Sciences of Animal Resources; 35; 1-9. doi: 10.5851/kosfa.2015.35.1.1.
Bressani, A. P. P., Martinez, S. J., Sarmento, A. B. I., Borém, F. M. & Schwan, R. F. (2020). Organic Acids Produced during Fermentation and Sensory Perception in Specialty Coffee using Yeast Starter Culture. Food Research International; 128; 108773. doi: 10.1016/j.foodres.2019.108773.
Chandramouli, Y., Gandhimati, R., Yasmeen, B. R., Vikram, A., Mahitha, B. & Imroz, S.M. (2004). Review On Cocrystal as An Approach with Newer Implication in Pharmaceutical Field. International Journal of Medicinal Chemistry and Analysis; 2; 91-100.
Chichlowski, M., German, J. B., Lebrilla, C. B. & Mills, D. A. (2011). The Influence of Milk Oligosaccharides on Microbiota of Infants: Opportunities for Formulas. Annual Review of Food Science and Technology; 2; 331-351. doi: 10.1146/annurev-food-022510-133743.
Da Mota, M. C. B., Batista, N. N., Rabelo, M. H. S., Ribeiro, D. E., Borém, F. M. & Schwan, R. F. (2020). Influence of Fermentation Conditions on the Sensorial Quality of Coffee Inoculated with Yeast. Food Research International; 136; 109482. doi: 10.1016/j.foodres.2020.109482.
Denkova, R., Goranov, B., Teneva, D., Denkova, Z. & Kostov, G. (2017). Antimicrobial Activity of Probiotic Microorganisms: Mechanisms of Interaction and Methods of Examination. Antimicrobial Research: Novel Bioknowledge and Educational Programs; 34; 201-212.
Evangelista, S. R., Miguel, M. G., de Souza Cordeiro, C., Silva, C. F., Pinheiro, A. C. & Schwan, R. F. (2014). Inoculation of Starter Cultures in a Semi-Dry Coffee (Coffea arabica) Fermentation Process. Food Microbiology; 44; 87–95.
Giannelli, F. R. (2017). PA-C Antibiotic-associated Diarrhea. Journal of the American Academy of Physician Assistants; 30; 46-47. doi: 10.1097/01.JAA.0000524721.01579.c9.
Gibson, G. R., Scott, K. P., Rastall, R. A., Tuohy, K. M., Hotchkiss, A., Dubert-Ferrandon, A., Gareau, M.,
Murphy, E. F., Saulnier, D. & Loh, G. (2010). Dietary Prebiotics: Current Status and New Definition. Food Science & Technology Bulletin Functional Foods; 7; 1–19.
Guarino, A., Guandalini, S. & Vecchio, A. (2015). Probiotics for Prevention and Treatment of Diarrhea. Journal of Clinical Gastroenterology; 49; 37-45. doi: 10.1097/MCG.0000000000000349.
Guimarí£es, A. C., Meireles, L. M., Lemos, M. F., Guimarí£es, M., Endringer, D. C., Fronza, M. & Scherer, R. (2019). Antibacterial Activity of Terpenes and Terpenoids Present in Essential Oils. Molecules; 24; 2471. doi: 10.3390/molecules24132471.
Haile, M. & Kang, W. H. (2019). The Role of Microbes in Coffee Fermentation and Their Impact on Coffee Quality. Journal of Food Quality; 2019; 1-16. doi: 10.1155/2019/4836709.
Holkem, A., Raddatz, G., Nunes, G., Cichoski, A., Jacob-Lopes, E., Grosso, C. & Menezes, C. (2016). Development and Characterization of Alginate Microcapsules Containing Bifidobacterium BB-12 Produced by Emulsification/Internal Gelation Followed by Freeze Drying. Food Science and Technology; 71; 302-308. doi: 10.1016/j.lwt.2016.04.012.
Kaczmarek, B. (2020). Tannic Acid with Antiviral and Antibacterial Activity as A Promising Component of Biomaterials-A Minireview. Materials; 13; 3224.
Liu, G., Song, Z., Yang, X., Gao, Y., Wang, C. & Sun, B. (2015). Antibacterial Mechanism of Bifidocin A, a Novel Broad-Spectrum Bacteriocin Produced by Bifidobacterium animalis BB04. Food Control; 62; 309-316. doi: 10.1016/j.foodcont.2015.10.033.
Louis, P., Flint, H. J. & Michel, C. (2016). How to Manipulate the Microbiota: Prebiotics. Advances in Experimental Medicine and Biology; 902; 119-142. doi: 10.1007/978-3-319-31248-4_9.
Macfarlane, G., Steed, H. & Macfarlane, S. (2008). Bacterial Metabolism and Health-Related Effects of Galacto-Oligosaccharides and Other Prebiotics. Journal of Applied Microbiology; 104; 305–344.
Mabhiza, D., Chitemerere, T. & Mukanganyama, S. (2016). Antibacterial Properties of Alkaloid Extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. International Journal of Medicinal Chemistry; 2016; 1-7. doi: 10.1155/2016/6304163.
Madsen, K. L. (2012). Enhancement of Epithelial Barrier Function by Probiotics. Journal of Epithelial Biology & Pharmacology; 5; 55–59.
Makras, L., Triantafyllou, V., Fayol-Messaoudi, D., Adriany, T., Zoumpopoulou, G., Tsakalidou, E., Servin, A. & DeVuyst, L. (2006). Kinetic Analysis of the Antibacterial Activity of Probiotic Lactobacilli Towards Salmonella enterica Serovar typhimurium Reveals a Role for Lactic Acid and Other Inhibitory Compounds. Research in Microbiology; 157; 241–247. doi: 10.1016/j.resmic.2005.09.002.
Marques, W., Raghavendran, V., Stambuk, B. U. & Gombert, A. K. (2016). Sucrose and Saccharomyces cerevisiae: A Relationship Most Sweet. Federation of European Microbiological Societies Yeast Research; 16; 1-16. doi: 10.1093/femsyr/fov107.
Mindarti, S., Zalizar, L., Damat, Saati, E. A. & Fajriani, S. (2020). Characterization of Fiber Fraction, Physical and Chemical Properties of Coffee Flour (Coffea sp.) as Functional Foodstuff for Diabetes Mellitus Patient. IOP Conferences Series: Earth and Environmental Science; 462; 1-10.
Mohammed, M., Raman, N., Alhoot, M. & Alwan, R. (2020). Antibacterial Activities of Allium sativum (Garlic) Extracts Against Staphylococcus aureus and Escherichia coli. European Journal of Molecular & Clinical Medicine; 7; 526-534.
Mohkam, M., Nezafat, N., Berenjian, A., Negahdaripour, M., Behfar, A. & Ghasemi, Y. (2016). Role of Bacillus genus
in the Production of Value-Added Compounds. Basel: Springer.
Osman, A., Tzortzis, G., Rastall, R. A. & Charalampopoulos, D. (2012). BbgIV is an Important Bifidobacterium β-galactosidase for the Synthesis of Prebiotic Galactooligosaccharides at High Temperatures. Journal of Agriculture and Food Chemistry; 60; 740–748.
Pandey, K. R., Naik, S. R. & Vakil, B. V. (2015). Probiotics, Prebiotics and Symbiotics - a Review. Journal of Food Science and Technology; 52; 7577–7587.
Pereira, G., Thomaz-Soccol, V., Pandey, A., Madeiros, A., Lara, J., Gollo, A. & Soccol, C. (2014). Isolation, Selection and Evaluation of Yeasts for Use in Fermentation of Coffee Beans by the Wet Process. International Journal of Food Microbiology; 188; 60-66. doi: 10.1016/j.ijfoodmicro.2014.07.008.
Plaza-Diaz, J., Ruiz-Ojeda, F. J., Gil-Campos, M. & Gil, A. (2019). Mechanisms of Action of Probiotics. Advances in nutrition; 10; 49–66. doi: 10.1093/advances/nmy063.
Pokusaeva, K., Fitzgerald, G. F. & van Sinderen, D. (2011). Carbohydrate metabolism in Bifidobacteria. Genes & Nutrition; 6; 285–306. doi: 10.1007/s12263-010-0206-6.
Riddle, M. S., DuPont, H. L. & Connor, B. A. (2016). ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults. American Journal of Gastroenterology; 111; 602-622. doi: 10.1038/ajg.2016.126.
Rizal, S., Murhadi, Kustyawati, M. A. & Hasanudin, U. (2020). Growth Optimization of Saccharomyces cerevisiae and Rhizopus oligosporus during Fermentation to Produce Tempeh with High β-glucan Content. Biodiversitas; 21; 2667-2673. doi: 10.13057/biodiv/d210639.
Rosburg, V., Boylston, T. & White, P. (2010). Viability of Bifidobacteria Strains in Yogurt with Added Oat Beta-Glucan and Corn Starch During Cold Storage. Journal of Food Science; 75; 439–444. doi: 10.1111/j.1750-3841.2010.01620.x.
Sarkar, A. & Mandal, S. (2016). Bifidobacteria - Insight into Clinical Outcomes and Mechanisms of Its Probiotic Action. Microbiological Research; 192; 159-171. doi: 10.1016/j.micres.2016.07.001.
Saulnier, D. M., Gibson, G. R. & Kolida, S. (2008). In Vitro Effects of Selected Symbiotics on the Human Faecal Microbiota Composition. Federation of European Microbiological Societies Microbiology & Ecology; 66; 516–527.
Silva, C. F., Vilela, D. M., de SouzaCordeiro, C., Duarte, W. F., Dias, D. R. & Schwan, R. F. (2013). Evaluation of a Potential Starter Culture for Enhances Quality of Coffee Fermentation. World Journal of Microbiology & Biotechnology; 29; 235–247. doi: 10.1007/s11274-012-1175-2.
Stiverson, J., Williams, T., Chen, J., Adams, S., Hustead, D., Price, P., Guerrieri, J., Deacon, J. & Yu Z. (2014). A Comparative Evaluation of Prebiotic Oligosaccharides using In Vitro Cultures of Infant Fecal Microbiome. Applied and Environmental Microbiology; 80; 7388-7397. doi: 10.1128/AEM.02200-14.
Symonds, E. L., O'Mahony, C., Lapthorne, S., O'Mahony, D., Sharry, J. M., O'Mahony, L. & Shanahan, F. (2012). Bifidobacterium infantis 35624 Protects Against Salmonella-Induced Reductions in Digestive Enzyme Activity in Mice by Attenuation of the Host Inflammatory Response. Clinical and Translational Gastroenterology; 3; 15.
Wang, X., Wang, J., Sun, H., Xia, S., Duan, R. & Liang, J. (2015). Etiology of Childhood Infectious Diarrhea in a Developed Region of China: Compared to Childhood Diarrhea in a Developing Region and Adult Diarrhea in a Developed Region. PLoS ONE; 10; 1-14. doi: 10.1371/journal.pone.0142136.
Wang, Y., Wu, J., Lv, M., Shao, Z., Hungwe, M., Wang, J., Bai, X., Xie, J., Wang, Y. & Geng, W. (2021). Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry. Frontiers in Bioengineering and Biotechnology; 9; 1-19. doi: 10.3389/fbioe.2021.612285.
Wulandari, S., Ainuri, M. & Sukartiko, A. C. (2021). Biochemical Content of Robusta Coffees Under Fully-Wash, Honey, and Natural Processing Methods. IOP Conferences Series: Earth and Environmental Science; 819; 1-9.
Xie, Y., Yang, W., Tang, F., Chen, X. & Ren, L. (2015). Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism. Current Medicinal Chemistry; 22; 132–149. doi: 10.2174/0929867321666140916113443.
Yang, J. & Yang, H. (2019). Antibacterial Activity of Bifidobacterium breve Against Clostridioides difficile. Frontiers in Cellular and Infection Microbiology; 9; 1-12. doi: 10.3389/fcimb.2019.00288.
Zhou, Y., Zhu, X., Hou, H. et al. (2018). Characteristics of Diarrheagenic Escherichia coli among Children Under 5 Years of Age with Acute Diarrhea: A Hospital Based Study. BMC Infectious Disease; 18; 1-10. doi: 10.1186/s12879-017-2936-1.
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