Analysis of Cayenne Pepper Fruit (Capsicum frutescens) in Inhibiting HMG-CoA Reductase Activity as a Treatment for Hypercholesterolemia
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Background: Hypercholesterolemia is a major cause of cardiovascular disease and its incidence continues to increase. Statins are a group of hypercholesterolemic therapies known to trigger various side effects; therefore, statin alternatives need to be investigated. The cayenne pepper (Capsicum frutescens) contains secondary metabolites that inhibit the activity of cholesterol-forming enzymes (HMG-CoA reductase). Objective: The aim of this study was to identify the ability of C. frutescens fruit to inhibit HMG-CoA reductase activity to prevent hypercholesterolemia. Methods: This was a true experimental study using a posttest-only control group design. The independent variables were n-hexane, methanol, and ethanol extracts of C. frutescens fruit, each with a concentration of 0.01%, with HMG-CoA reductase activity as the dependent variable. Enzymatic activity was measured enzymatically using spectrometry. Results: The mean values of % inhibition from n-hexane, methanol, and ethanol extracts of C. frutescens and pravastatin were 95.74%, 104.70%, 100.11%, and 99.27%, respectively. The average specific activities of n-hexane, methanol, and ethanol extracts of C. frutescens and pravastatin were 0.5765, 0.6029, 0.5513, and 0.5716 units/mgP, respectively. There was a significant difference between the sample groups in the inhibition of HMG-CoA reductase activity. HMG-CoA reductase inhibitory activity was highest in the methanol extract, followed by the n-hexane extracts. The activity of these extracts was higher than that of pravastatin alone. Conclusion: The methanol extract showed the best inhibitory activity. C. frutescens has been shown to have great potential in inhibiting the activity of the enzyme HMG-CoA reductase and preventing hypercholesterolemia.
Bastianini, M., Faffa, C., Sisani, M., & Petracci, A. (2018). Caffeic acid-layered double hydroxide hybrid: A new Aqeel, M. T., Ur-Rahman, N., Khan, A. U., Ashraf, Z., Latif, M., Rafique, H., Rasheed, U. (2018). Antihyperlipidemic Studies of Newly Synthesized Phenolic Derivatives: In Silico And In Vivo Approaches. Drug Design, Development, and Therapy, 12: 2443–2453. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6089105/.
Arifin, B., Ibrahim, S. (2018). Struktur, Bioaktivitas Dan Antioksidan Flavonoid. Jurnal Zarah, 6(1): 21-29.
Azmi, M. B., Sultana, S., Naeem, S., Qureshi, S. A. (2021). In silico investigation on alkaloids of Rauwolfia serpentina as potential inhibitors of 3-hydroxy-3-methyl-glutaryl-CoA reductase. Saudi Journal of Biological Sciences, 28(1): 731–737.. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783793/
Bansal, A. (2021). Medical Reference HMG-CoA Reductase Inhibitor. https://www.statpearls.com/ArticleLibrary/viewarticle/22953
Baskaran, G., Salvamani, S., Ahmad, S. A., Shaharuddin, N. A., Pattiram, P. D., Shukor, M. Y. (2015). HMG-CoA Reductase Inhibitory Activity And Phytocomponent Investigation Of Basella Alba Leaf Extract As a Treatment For Hypercholesterolemia. Drug Design, Development and Therapy, 9: 509-517.
Dewi, I. P., Merry., M. S. (2017). Peranan Obat Golongan Statin. Berkala Ilmiah Kedokteran Duta Wacana, 2(3).
Djarkasi, S. G. S., Raharjo, S., Noor, Z. (2017). Isolasi Dan Akitivitas Spesifik Enzim Lipase Indigenous Biji Kenari. Jurnal Teknologi Pertanian, 8(1): 28-35.
Farida, Y., Putri, C. (2016). Efek Penggunaan Simvastatin Terhadap Kenaikan Gula Darah Puasa Pasien Diabetes Melitus Tipe 2. Journal Of Pharmaceutical Science And Clinical Research, 1: 58-65.
Feng, J., Liu, H., Yang, X., Gao, A., Liao, J., Feng, L. (2013). Comparison of Activity Indexes For Recognizing Enzyme Mutants Of Higher Activity With Uricase As Model. Chemistry Central Journal, 7(69) : 1-8.
Fridiana, Slamet, Arifiyanti, D., Wirasti. (2019). Uji Aktivitas Antioksidan Partisi n-Heksan, Metanol, Dan Ekstrak Buah Cabai Rawit (Capsicum frutescens L.) Dengan Metode FRAP (Ferric Reducing Antioxidant Power). e-Skripsi UMPP, 2(1): 56-75.
Gesto, D. S., Pereira, C. M. S., Cerqueira, N. M. F. S., Sousa, S. F. (2020). An Atomic-Level Perspective of HMG-CoA-Reductase: The Target Enzyme to Treat Hypercholesterolemia. Molecules : 3891(25).
Giovedi, K. (2016). Penetapan Kadar Kapsaisin Dan Uji Aktivitas Antioksidan Fraksi Toluen-Etil Asetat Buah Cabai Rawit (Capsicum frutescens) Dengan Metode 2,2-Difenil-1-Pikrilhidrazil (DPPH). Skripsi. Fakultas Farmasi Universitas Sanata Dharma. Yogyakarta.
Hariyanti, Rachmania, R. A., Karinah, M., Sunaryo, H. (2018). In Silico Analysis of the Phytochemical Compounds in Carica papaya Seeds for Optimizing the Inhibitors of HMG-CoA Reductase. Scitepress, 123-132.
Islam, B., Sharma, C., Adem, A., Aburawi, E., Ojha, S. (2015). Insight Into The Mechanism Of Polyphenols On The Activity Of HMGR By Molecular Docking. Drug Design, Development and Therapy, 2015:9.
Jempormase, F., Bodhi, W., Kepel, B. J. (2016). Prevalensi Hiperkolesterolemia Pada Remaja Obes Di Kabupaten Minahasa. Jurnal e-Biomedik (eBm), 4(1): 25-29.
Kopjar, M., Lončarić, A., Mikulinjak, M., Šrajbek, Z., Šrajbek, M., Pichler A. (2016). Evaluation of Antioxidant Interactions of Combined Model Systems of Phenolics in the Presence of Sugars. Natural Product Communications, 11(10): 1445 -1448.
Marahatha, R., Basnet, S., Bhattarai, B. R., Budhathoki, P., Aryal, B., Adhikari, B. (2021). Potential Natural Inhibitors Of Xanthine Oxidase And HMG-CoA Reductase In Cholesterol Regulation: In Silico Analysis. BMC Complementary Medicine and Therapies, 21(1).
Mannino, G., Iovino, P., Lauria, A., Genova, T., Asteggiano, A., Notarbartolo, A. (2021). Bioactive Triterpenes of Protium heptaphyllum Gum Resin Extract Display Cholesterol-Lowering Potential. International Journal of Molecular Science, 22(5): 2664. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961947/
Melannisa, R., Trisharyanti, I., Suhendi, A., Da’i, M., Atmaja, A. I. K. (2011). Pharmacon. Pharmaceutical Journal of Indonesia, 12(2): 60-64.
Munira, Utami, K., Nasir, M. (2019). Uji Aktivitas Antibakteri Cabai Rawit Hijau Dan Cabai Rawit Merah (Capsicum frutescens) Serta Kombinasinya Terhadap Bakteri Staphylococcus aureus. Jurnal Bioleuser, 3(1): 13-17.
Murray, R..K., Bender, D.A., Botham, K.M., Kennelly, P.J., Rodwell, V.W., Weil, P.A. (2012). Biokimia Harper Edisi 27. Jakarta: Penerbit Buku Kedokteran EGC.
Nascimento, P. L. A. D., Nascimento, T. C. E. S., Ramos, N. S. M., Silva, G. R. D., Camara, C. A., Silva, T. M. S., Moreira, K. A., Porto, A. L. F. (2013). Antimicrobial And Antioxidant Activities of Pimenta Malagueta (Capsicum frutescens). African Journal of Microbiology Research, 7(27): 3526-3533.
Nematollahi, A., Aminimoghadamfarouj, N., Jalilvand, M. R., Vakili, S. A. (2012). Design and molecular docking studies of luteolin derivatives, from Biebersteinia multifida DC., as novel HMG-CoA reductase inhibitors. International Journal of ChemTech Research, 4(2): 733-738.
Rahim, A., Nurmayanti, W. P. (2020). Aktivitas Antibakteri Fraksi Daun Cabe Rawit (Capsicum frutescens) Terhadap Bakteri Staphylococcus aureus. Pharmacoscript, 3(2): 134-142.
Rizqi, A. W. S., Widada, S. T. , Martsiningsih ,M. A. (2014). Pengaruh Pemberian Jus Buah Naga Merah (Hylocereus polyrhizus) Terhadap Kadar Trigliserida Tikus Putih (Rattus norvegicus) Hiperlipidemia. Jurnal Teknologi Laboratorium, 3(2).
Rivera, M. L. C., Hassimotto, N. M. A., Bueris, V., Sircili, M. P., Almeida, F. A. D., Pinto, U. M. (2019). Effect of Capsicum Frutescens Extract, Capsaicin, and Luteolin on Quorum Sensing Regulated Phenotypes. Journal of Food Science, 1-10.
Saripa, J., Hasanuddin, S., Isrul, M. (2020). Aktivitas Antimikroba Ekstrak Etanol Daun Cabai Rawit Spesies Capsicum frutescens Linn dan Capsicum annum pada Staphylococcus aureus. Jurnal Mandala Pharmacon Indonesia, 6(2): 104-110.
Shaik, A. H., Shaik, S. R., Daddam, J. R., Ali, D., Manoharadas, S., Arafah, M. W., Kodidhela, L. D. (2020). Maslinic Acid And Gallic Acid Protective Efficacy On Lipids, Lipoproteins And Lipid Metabolizing Enzymes Against Isoproterenol Administered Cardiotoxicity: An In Vivo And In Silico Molecular Docking Evidences. Journal of King Saud University, 33.
Shen, C., Huang, L., Xiang, H., Deng M., Gao, H., Zhu, Z. (2016). Inhibitory Effects On The HMG-Coa Reductase In The Chemical Constituents of The Cassia Mimosoides Linn. Revista Română De Medicină De Laborator. 24(4): 413-422.
Simpson, M. G. (2010). Plant Systematics. 2nd Ed. Elsevier Science. Burlington.
Tavadyan, L. A., Minasyan, S. H. (2019). Synergistic And Antagonistic Co-Antioxidant Effects of Flavonoids With Trolox Or Ascorbic Acid In A Binary Mixture. J. Chem. Sci, 131(40).
Talitha, Z. A. (2017). Aktivitas Antioksidan Ekstrak Cabai Rawit (Capsicum frutescens) Metode Modified-Microwave Assisted Extraction (MAE) (Kajian Genotip Cabai Dan Lama Waktu Ekstraksi). Skripsi. Jurusan Teknologi Hasil Pertania Fakultas Tekonologi Pertanian Universitas Brawijaya. Malang.
Taolin, C. (2019). Efek Antimikroba Capsaicin. Jurnal Ilmiah Kesehatan Sandi Husada, 10(2): 212-216.
Ward, N. C., Watts, G. F., Eckel, R. H. (2019). Statin Toxicity Mechanistic Insights and Clinical Implications. American Heart Association, 124: 328-350.
Wijaya, S., Yonas, S. M. K., Hartanti, L., Setiawan, H. K., Soegianto, L. (2018). Studi pendahuluan: Korelasi Aktivitas Antikolesterol Dengan Aktivitas Antioksidan Ekstrak Etanol Daun Salam (Syzygium polyanthum). Journal Of Pharmacy Science And Practice, 5(2): 100-111.
World Health Organization. (2011). Global Atlas on Cardiovaskular Disease Prevention and Control. https://apps.who.int/iris/handle/10665/44701raw material for cosmetic applications. Cosmetics, 5(3). https://doi.org/10.3390/COSMETICS5030051
Chen, C., Zhou, M., Ge, Y., & Wang, X. (2020). SIRT1 and aging related signaling pathways. In Mechanisms of Ageing and Development (Vol. 187). Elsevier Ireland Ltd. https://doi.org/10.1016/j.mad.2020.111215
Chen, S., Liu, J., Dong, G., Zhang, X., Liu, Y., Sun, W., & Liu, A. (2021). Flavonoids and caffeoylquinic acids in Chrysanthemum morifolium Ramat flowers: A potentially rich source of bioactive compounds. Food Chemistry, 344. https://doi.org/10.1016/j.foodchem.2020.128733
Cizmarova, B., Hubkova, B., Bolerazska, B., Marekova, M., & Birkova, A. (2020). Caffeic acid: A brief overview of its presence, metabolism, and bioactivity. Bioactive Compounds in Health and Disease, 3(4), 74–81. https://doi.org/10.31989/bchd.v3i4.692
Dutta, R. K., Lee, J. N., Maharjan, Y., Park, C., Choe, S. K., Ho, Y. S., Kwon, H. M., & Park, R. (2022). Catalase-deficient mice induce aging faster through lysosomal dysfunction. Cell Communication and Signaling, 20(1). https://doi.org/10.1186/s12964-022-00969-2
Firdayeni, I. G. A. R. M., & Sari, P. M. N. A. (2022). Review Artikel Potensi Limbah Kulit Kopi (Coffea sp.) sebagai Bahan Baku pada Produk Kosmetik Anti-Aging. Prosiding Workshop dan Seminar Nasional Farmasi (Vol. 1, Issue 1).
Grabowska, W., Sikora, E., & Bielak-Zmijewska, A. (2017). Sirtuins, a promising target in slowing down the ageing process. Biogerontology,18(4)447–476. ds. https://doi.org/10.1007/s10522-017-9685-9
Hartanto, R., Fitri, S. R., Kawiji, K., Prabawa, S., Sigit, B., & Yudhistira, B. (2021). Analisis Fisik, Kimia dan Sensoris Teh Bunga Krisan Putih (Chrysanthemum morifolium Ramat.) dengan Pengeringan Kabinet. Agrointek : Jurnal Teknologi Industri Pertanian, 15(4), 1011–1025. https://doi.org/10.21107/agrointek.v15i4.10531
Hilal, N., Syahrir, A., Mochamad Afendi, F., Susetyo, B., Mochamad, F., & Program, A. (2016). Efek Sinergis Bahan Aktif Tanaman Obat Berbasiskan Jejaring Dengan Protein Target, Jurnal Jamu Indonesia, 1(1).
Kamarudin, A. A., Sayuti, N. H., Saad, N., Razak, N. A. A., & Esa, N. M. (2021). Eleutherine bulbosa (Mill.) urb. bulb: Review of the pharmacological activities and its prospects for application. International Journal of Molecular Sciences,22(13). https://doi.org/10.3390/ijms22136747
Kumalasari, E., & Prihandiwati, E. (2019). Formulasi Krim Antiaging Ekstrak Daun Bawang Dayak dengan Emulgator Anionik dan Nonionik. Jurnal Insan Farmasi Indonesia, 2(2), 222–230. https://doi.org/10.36387/jifi.v2i2.386
Lin, L. Z., & Harnly, J. M. (2010). Identification of the phenolic components of chrysanthemum flower (Chrysanthemum morifolium Ramat). Food Chemistry, 120(1), 319–326. https://doi.org/10.1016/j.foodchem.2009.09.083
Ma, D., & Wako, Y. (2017). Evaluation of phenolic compounds and neurotrophic/neuroprotective activity of cultivar extracts derived from Chrysanthemum morifolium flowers. Food Science and Technology Research, 23(3), 457–467. https://doi.org/10.3136/fstr.23.457
Marlina, A., & Widiastuti, E. (2021). Studi Awal Pembuatan Bio-Insektisida dari Bunga Krisan (Chrysantemum Morrifolium). Prosiding The 12th Industrial Research Workshop and National Seminar. 12, 128-160.
Mokoginta, R. V, Simbala, H. E. I., & Mansauda, K. L. R. (2020). Uji Aktivitas Antioksidan Ekstrak Etanol Bulbus Bawang Dayak (Eleutherine americana Merr) dengan Metode DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Antioxidant Activity Test of Ethanol Extracts Dayak Onion Bulbs (Eleutherine americana Merr) with DPPH Method (1,1-Diphenyl-2-Picrylhydrazyl). Phamacon 9(3).
Muti’ah, R., Listiyana, A., Nafisa, B. B., & Suryadinata, A. (2020). Kajian Efek Ekstrak Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr) sebagai Antikanker. Jurnal of Islamic Pharmacy,5(2).
Nailufa, Y., & Najih, Y. A. (2020). Formulasi Krim Epigallocatechin gallate Sebagai Anti Aging. Journal of Pharmacy and Science, 5(2), 81–85. https://doi.org/10.53342/pharmasci.v5i2.186
Narko, T., Permana, B., Prasetiawati, R., Soni, D., Khairiyah, F., Au, L., & Sastranegara, L. H. (2017). Molecular Docking study of Bulb of Bawang Dayak (Eleutherine Palmifolia (L) Merr ) Compound as Anti Cervical Cancer. Jurnal Ilmiah Farmako Bahari, 8(2), 1–14. http://PubChem.ncbi.nlm.nih.gov
Novaryatiin, S., Ramli, A., Dian Ardhany, S., Pengajar Program Studi DIII Farmasi, D., Ilmu Kesehatan, F., Muhammadiyah Palangkaraya, U., & Program Studi DIII Farmasi, M. (2019). Uji Daya Hambat Ekstrak Etanol Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) terhadap Bakteri Staphylococcus aureus. Jurnal Surya Medika,4(2).
Permatasari, G. W., Atho’illah, M. F., & Putra, W. E. (2021). Target protein prediction of Indonesian jamu kunyit asam (Curcumin-tamarind) for dysmenorrhea pain reliever: A network analysis approach. Jurnal Kedokteran Dan Kesehatan Indonesia. https://doi.org/10.20885/jkki.vol12.iss3.art7
Pramiastuti, O., Ika, D., Solikhati, K., Suryani, A., S1, P., Stikes, F., Mandala, B., & Slawi, H. (2021). Aktivitas Antioksidan Fraksi Umbi Bawang Dayak (Eleutherine bulbosa (Mill.) Urb) dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil) Antioxidant Activity of Fractions Dayak Onion Bulbs (Eleutherine bulbosa (Mill.) Urb) BY DPPH(1,1-diphenyl-2-picrylhidrazil) Method. Jurnal Wiyata, 8(1), 55–65.
Puspita, D., Nugroho, P., Titania, M. C., Putri, A., Pangan, E. T., Kedokteran, F., Kesehatan, I., Kristen, U., Wacana, S., Kartini, J. L., & 11, N. (2023). Optimizing the Drying Temperature of Bloom Tea Using Oven and Microwave for Conservation of Pigment and Antioxidant Content. Science, Technology and Management Journal, 3(1), 10–14. https://doi.org/10.26623/jtphp.v13i1.1845.kodeartikel
Qomariasih, N., Susetyo, B., & Afendi, F. M. (2016). Analisis Gerombol Simultan dan Jejaring Farmakologi antara Senyawa dengan Protein Target pada Penentuan Senyawa Aktif Jamu Anti Diabetes Tipe 2. Jurnal Jamu Indonesia, 1(2).
Qureshi, M. A., & Javed, S. (2022). Investigating binding dynamics of trans resveratrol to HSA for an efficient displacement of aflatoxin B1 using spectroscopy and molecular simulation. Scientific Reports, 12(1). https://doi.org/10.1038/s41598-022-06375-5
Rahmadiani, N. F., & Hasanah, A. N. (2019). Formulasi dan Evaluasi Sediaan Anti Aging dari Ekstrak Tumbuhan. Farmasetika.Com (Online), 4(4). https://doi.org/10.24198/farmasetika.v4i4.23068
Rosyadah, M., Afendi, F. M., & Kusuma, W. A. (2017). Penguraian Mekanisme Kerja Jamu dengan Menggunakan Analisis Graf Tripartit pada Jejaring Senyawa-Protein-Penyakit. Jurnal Jamu Indonesia, 2(1). http://pharmgkb.org
Saputra, D. E., Handayani, N., & Wartono, M. W. (2016). Isolation and Identification of Β-Sitosterol and Stigmasterol Mixture From Root Bark of Slatri (Calophyllum soulattri Burm. f). ALCHEMY Jurnal Penelitian Kimia, 10(1), 87. https://doi.org/10.20961/alchemy.v10i1.14
Sembiring, E. K. D., Sulistyaningsih, E., & Shintiavira, H. (2021). Pengaruh Berbagai Konsentrasi Giberelin (GA3) terhadap Pertumbuhan dan Hasil Bunga Krisan (Chrysanthemum morifolium L.) di Dataran Medium. Vegetalika, 10(1), 44. https://doi.org/10.22146/veg.47856
Siswanto, F. M., & Kartiko, B. H. (2017). Aplikasi Teknologi Crispr/Cas9 Dalam Anti-Aging Medicine. Jurnal Media Sains, 1(2), 50–56.
Tjandrawinata, R. R., Amalia, A. W., Tuna, H., Saidi, V. N., & Tan, S. (2022). Molecular Mechanisms of Network Pharmacology-Based Immunomodulation of Huangqi (Astragali Radix). Jurnal Ilmu Kefarmasian Indonesia, 20(2). https://www.ncbi.nlm.nih.gov/gene/
Trist, B. G., Hilton, J. B., Hare, D. J., Crouch, P. J., & Double, K. L. (2021). Superoxide Dismutase 1 in Health and Disease: How a Frontline Antioxidant Becomes Neurotoxic. Angewandte Chemie - International Edition, 60(17), 9215–9246). https://doi.org/10.1002/anie.202000451
Wahdaningsih, S., Rizkifani, S., Untari, E. K., & Rinaldi, W. (2023). Effect of Drying Method on Levels of Antioxidant Activity, Total Flavonoid Levels, and Total Phenol Levels in Ethanol Extract of Bawang Dayak (Eleutherine americana) Leaves. Majalah Obat Tradisional, 28(1), 37–39. https://doi.org/10.22146/mot.80085
Wang, Y., Sun, J., Ma, D., Li, X., Gao, X., Miao, J., & Gao, W. (2019). Improving the contents of the active components and bioactivities of Chrysanthemum morifolium Ramat.: The effects of drying methods. Food Bioscience, 29, 9–16. https://doi.org/10.1016/j.fbio.2019.03.003
Wanita, Y. P. (2022). Potensi Produk Samping Budidaya Krisan sebagai Minuman Fungsional: Senyawa Kimia dan Nilai Tambahnya Potential Side Products of Cultivation of Chrysanthemum as a Functional Beverage: Chemical Compounds And Its Value Added. Jurnal Pertanian Agros, 24(2).
Xu, J., Su, X., Burley, S. K., & Zheng, X. F. S. (2022). Nuclear SOD1 in Growth Control, Oxidative Stress Response, Amyotrophic Lateral Sclerosis, and Cancer. Antioxidants,11(2). https://doi.org/10.3390/antiox11020427
Yuan, H., Jiang, S., Liu, Y., Daniyal, M., Jian, Y., Peng, C., Shen, J., Liu, S., & Wang, W. (2020). The flower head of Chrysanthemum morifolium Ramat. (Juhua): A paradigm of flowers serving as Chinese dietary herbal medicine. Journal of Ethnopharmacology, 261https://doi.org/10.1016/j.jep.2020.113043
Zhang, J., Liu, X., Pan, J., Zhao, Q., Li, Y., Gao, W., & Zhang, Z. (2020). Anti-aging effect of brown black wolfberry on Drosophila melanogaster and D-galactose-induced aging mice. Journal of Functional Foods, 65. https://doi.org/10.1016/j.jff.2019.103724
Zhou, H., Zhang, X., Li, B., & Yue, R. (2023). Fast and efficient identification of hyaluronidase specific inhibitors from Chrysanthemum morifolium Ramat. using UF-LC-MS technique and their anti-inflammation effect in macrophages. Heliyon, 9(2). https://doi.org/10.1016/j.heliyon.2023.e13709
Zhou, Z., Chen, B., Chen, S., Lin, M., Chen, Y., Jin, S., Chen, W., & Zhang, Y. (2020). Applications of Network Pharmacology in Traditional Chinese Medicine Research. Evidence-based Complementary and Alternative Medicine, 2020. https://doi.org/10.1155/2020/1646905
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