Phytochemical Screening and Anti-hyperglycemic Effect Test of Ethanol Extract of Waru Leaf (Hibiscus tiliaceus) on Glucose-loaded Mice
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Background: The treatment of diabetes mellitus relies on synthetic drugs with various side effects. Therefore, it is necessary to explore alternative treatments with herbal therapies, such as Hibiscus tiliaceus leaves. Objectives: The aim of this study was to determine the anti-hyperglycemic effect of an ethanol extract from Hibiscus tiliaceus leaves in glucose-loaded mice. Methods: The initial stage of dried leaf characterization was to ensure the identity, quality, purity, and safety to be used, and then extracted using the maceration method with 70% ethanol solvent. The next step was phytochemical screening to identify secondary metabolite content. The anti-hyperglycemic effect was evaluated using the oral glucose tolerance test (OGTT) on 25 male mice divided into five treatment groups. The negative control group was given Na CMC 0.5% w/v Na CMC, and the positive control group was administered glibenclamide. The ethanol extract of Hibiscus tiliaceus leaves was administered at doses of 200, 400, and 800 mg/kg. Approximately 200 µL of blood was collected and analyzed for glucose levels. The data were statistically analyzed using one-way analysis of variance (ANOVA) with SPSS ver. 25 program. Results: Hibiscus tiliaceus leaves contain alkaloids, flavonoids, saponins, and tannins. The highest decrease in blood glucose levels was observed in the ethanol extract group at a dose of 400 mg/kg BW, with a decrease of 78.52%, followed by a dose of 200 mg/kg BW of 76.63%, a dose group of 800 mg/kg BW of 73.48%, and a positive control group (glibenclamide) of 34.68%, which was significantly different from the negative control group (Na CMC 0.5%) (p < 0.05). Conclusion: The ethanol extract of H. tiliaceus has anti-hyperglycemic effects.
Ajebli, M., & Eddouks, M. (2019). The promising role of plant tannins as bioactive anti-diabetic agents. Current medicinal chemistry, 26(25), 4852-4884.
Andriani, Y., Sababathy, M., Amir, H., Sarjono, P. R., Syamsumir, D. F., Sugiwati, S., & Kassim, M. N. I. (2020,). The potency of Hibiscus tiliaceus leaves as antioxidant and anticancer agents via induction of apoptosis against MCF-7 cells. In IOP Conference Series: Materials Science and Engineering (Vol. 959, No. 1, p. 012022). IOP Publishing.
Dhanya, R., Arya, A. D., Nisha, P., & Jayamurthy, P. (2017). Quercetin, a lead compound against type 2 diabetes ameliorates glucose uptake via AMPK pathway in skeletal muscle cell line. Frontiers in Pharmacology, 8, 336.
He, Y., Hu, Z., Li, A., Zhu, Z., Yang, N., Ying, Z., & Cheng, S. (2019). Recent advances in biotransformation of saponins. Molecules, 24(13), 2365.
Huang, Q., Chai, W. M., Ma, Z. Y., Ou-Yang, C., Wei, Q. M., Song, S., & Peng, Y. Y. (2019). Inhibition of α-glucosidase activity and non-enzymatic glycation by tannic acid: Inhibitory activity and molecular mechanism. International journal of biological macromolecules, 141, 358-368.
Hidayah, A. N., Amananti, W., & Febriyanti, R. (2021). Skrining Fitokimia Daun Waru (Hibiscus Tiliaceus) di Kawasan Brebes, Tegal, dan Pemalang (Doctoral dissertation, Politeknik Harapan Bersama Tegal).
Jaitak, V. (2019). A review on molecular mechanism of flavonoids as anti-diabetic agents. Mini Reviews in Medicinal Chemistry, 19(9), 762-786.
Jeepipalli, S. P., Du, B., Sabitaliyevich, U. Y., & Xu, B. (2020). New insights into potential nutritional effects of dietary saponins in protecting against the development of obesity. Food chemistry, 318, 126474.
Keller, A. C., He, K., Brillantes, A. M., & Kennelly, E. J. (2021). A characterized saponin-rich fraction of Momordica charantia shows anti-diabetic activity in C57BLK/6 mice fed a high fat diet. Phytomedicine Plus, 1(4), 100134.
Kemenkes RI. (2017). Farmakope Herbal Indonesia, edisi II.
Li, F., Xie, W., Ding, X., Xu, K., & Fu, X. (2024). Phytochemical and pharmacological properties of the genus Tamarix: a comprehensive review. Archives of Pharmacal Research, 1-32.
Lolok, N., Awaliyah, N., & Astuti, W. (2020). Formulasi Dan Uji Aktivitas Sediaan Sabun Cair Pembersih Kewanitaan Ekstrak Daun Waru (Hibiscus tiliaceus) Terhadap Jamur Candida albicans. Jurnal Mandala Pharmacon Indonesia, 6(01): 59-60.
Ma, H., He, K., Zhu, J., Li, X., & Ye, X. (2019). The anti-hyperglycemia effects of Rhizoma Coptidis alkaloids: A systematic review of modern pharmacological studies of traditional herbal medicine. Fitoterapia, 134, 210-220.
Meilina, R., Yassirly, Kesumawati, Dhirah, U. H., & Rezeki, S. (2022). Antidiabetes Ekstrak Daun Kembang Sepatu pada Mencit (Mus musculus). Journal of Healtcare Technology and Medicine, 8(2), 1641-1654.
Naenggolan , L. U., Soleha, T. U., & Oktaba, Z. (2023). Review Article: Aktivitas Farmakologi Tumbuhan Waru (Hibiscus tiliaceus). Medula, 4(1), 615-620.
Putri, A. K., Susanto, L., Rahayu, A., & Setyaningrum, L. (2024). Pengaruh Pelarut Metanol dan Etanol terhadap Kadar Flavonoid Total Ekstrak Kulit Pohon Waru (Hibiscus tiliaceus L.). Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 5(2), 119-125.
Sanvee, S., Simalou, O., Tchani, G. W., Kagnou, H., Bakoma, B., Metowogo, K., ... & Kpegba, K. (2020). Anti-diabetic activity of tannin fraction of Bridelia ferruginea (Benth) leaf extract on fructose-induced diabetic mice. Journal of Herbmed Pharmacology, 10(1), 68-74.
Suarantika, F., & Patricia, V. M. (2023). Kandungan Fitokimia dan Aktivitas Antioksidan Tanaman Waru (Hibiscus tiliaceus L.). Journal of Pharmaceutical and Health Research, 4(1), 159-162.
Surahmaida, S., Rachmawati, A., & Handayani, E. (2020). Kandungan senyawa kimia daun waru (Hibiscus tiliaceus) di kawasan lingkar Timur Sidoarjo. Journal Pharmasci, 5(2), 39-42.
Tang, D., Chen, Q. B., Xin, X. L., & Aisa, H. A. (2017). Anti-diabetic effect of three new norditerpenoid alkaloids in vitro and potential mechanism via PI3K/Akt signaling pathway. Biomedicine & Pharmacotherapy, 87, 145-152.
Wang, P., Ding, X., Kim, H., Michalek, S. M., & Zhang, P. (2020). Structural effect on adjuvanticity of saponins. Journal of medicinal chemistry, 63(6), 3290-3297.
Yang, D., Wang, L., Zhai, J., Han, N., Liu, Z., Li, S., & Yin, J. (2021). Characterization of antioxidant, α-glucosidase and tyrosinase inhibitors from the rhizomes of Potentilla anserina L. and their structure–activity relationship. Food Chemistry, 336, 127714.
Zebua, N. F., Bahrianur, R., & Suwailim, S. (2024). Antioxidant and Antimicrobial Activities of Leaves of Medicinal Plant Hibiscus tiliaceus L. FARMASI Journal, 4(2), 123-130.
Zhou, Y., & Xu, B. (2023). New insights into anti-diabetes effects and molecular mechanisms of dietary saponins. Critical Reviews in Food Science and Nutrition, 63(33), 12372-12397.
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