Anti-Inflammatory And Anti-Oxidant Activities Of Glochidion Daltonii Branch Extract
Downloads
Background: Glochidion daltonii (MÜll. Arg.) Kurz, Euphorbiaceae family, is a native medicinal plant in tropical regions of Asia. In Thailand, it has traditionally been used for treating pain and mouth inflammation. Objective: This study aims to investigate the anti-inflammatory and anti-oxidant effects of G. daltonii ethanolic extract (GDE) both in vitro and in vivo. Method: The anti-inflammatory mechanism was examined in E.coli LPS-stimulated RAW264.7 cells by using semi-quantitative-reverse transcription-polymerase chain reaction and the potential anti-inflammatory effect of GDE was evaluated in Sprague-Dawley rat using carrageenan-induced rat paw edema method. In addition, DPPH assay was used for anti-oxidant evaluation. Result: The results showed that GDE at 0.063 - 0.250 mg/mL concentrations inhibited the expressions of tumor necrosis factor-α and interleukin-1β. Moreover, an intraperitoneal administration of GDE significantly reduced paw edema in rats. Furthermore, GDE showed high anti-oxidant property with IC50 values at 6.35 ± 0.28 µg/mL. Conclusion: The results support the traditionally use of GDE for treating inflammation.
Aggarwal, B. B. (2004). Nuclear factor-kappa B: the enemy within. Cancer Cell; 6; 203–208.
Balkwill, F. (2009). Tumor necrosis factor and cancer. Nature Reviews Cancer; 9; 361–371.
Chi, Y. S., Cheon, B. S. & Kim, H. P. (2001). Effect ofwogonin, a plant flavone from Scutellaia radix, on the suppression of cyclooxygenase-2 and the induction of inducible nitric oxide synthase in lipopolyssacharide-treatedRAW264.7 cells. Biochemical Pharmacology; 61; 1195–1203.
Guay, J., Bateman, K., Gordon, R., Mancini, J. & Riendeau, D. (2004). Carrageenan-induced paw edema in rat elicits a predominant prostaglandinE2 (PGE2) response in the central nervous system associated with the induction of micro somal PGE2 synthase-1. Journal of Biological Chemistry; 279; 24866–24872.
Kagiwada, K., Chida, D., Sakatani, T., Asano, M., Nambu, A., Kakuta, S. & Iwakura, Y. (2004). Interleukin (IL)-6, but not IL-1, induction in the brain downstream of cyclooxygenase-2 is essential for the induction of febrile response against peripheral IL-1α. Endocrinology; 145; 5044–5048.
Kolls, J. K. (2006). Oxidative stress in sepsis: a redox redux. Journal of Clinical Investigation; 116; 860-863.
Liu, X., Zhao, M., Wang, J., Yang, B. & Jiang, Y. (2008). Antioxidant activity of methanolic extract of emblica fruit (Phyllanthus emblica L.) from six regions in China. Journal of Food Composition and Analysis; 21; 219-228.
Loram, L. C., Fuller, A., Fick, L. G., Cartmell, T., Poole, S. & Mitchell, D. (2007). Cytokine profiles during carrageenan-induced inflammatory hyperalgesia in rat muscle and hind paw. Journal of Pain; 8; 127–136.
Mantovani, A., Allavena, P., Sica, A. & Balkwill, F. (2008). Cancer-relatedinflammation. Nature; 454; 436–444.
Morris, C. J. (2003). Carrageenan-induced paw edema in the rat and mouse. Methods in Molecular Biology; 225; 115–121.
Mosmann T. (1983). Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assay. Journal of Immunological Methods; 65; 55-63.
Shimada, K., Fujikawa, K., Yahara, K. & Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry; 40; 945–948.
Singleton, V. L., Orthofer, R. & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods in Enzymology; 299; 152-178.
Sripanidkulchai, B., Junlatat, J., Wara-aswapati & N., Hormdee, D. (2009). Anti-inflammatiory effect of Streblus asper leaf extract in rats and its modulation on inflammation-associated genes expression in RAW264.7 macrophage cells. Journal of Ethnopharmacology; 124; 566-570.
Sugawara, I., Yamada, H. & Mizuno, S. (2003). Relative importance of STAT4 in murine tuberculosis. Journal of Medical Microbiology; 52; 29–34.
Won, J. H., Im, H. T., Kim, Y. H., Yun, K. J., Park, H. J., Choi, J. W. & Lee, K. T. (2006). Anti- inflammatory effect of buddlejasaponinIV through the inhibition of iNOS and COX-2 expression in RAW264.7 macrophages via the NF-kappaB inactivation. British Journal of Pharmacology; 148; 216–225.
1. The copyright of this journal belongs to the Editorial Board and Journal Manager with the author’s knowledge, while the moral right of the publication belong to the author.
2. The formal legal aspect of journal publication accessibility refers to the Creative Commons Attribution-Non-Commercial-Share Alike (CC BY-NC-SA), which implies that the publication can be used for non-commercial purposes in its original form.
3. Every publication (print/electronic) is open access for educational, research, and library purposes. In addition to the objectives mentioned above, the editorial board is not responsible for copyright infringement
.jpg)
