Pathology Anatomy and Hemolymph Profile of Spiny Lobster (Panulirus homarus) Infested by Octolasmis sp. on Bottom Cage
Downloads
Spiny lobster, a fishery commodity with important economic value, is often infested by Octolasmis sp. on their bodies, which causes stress conditions that lead to death. This study aimed to determine the anatomical pathology and hemolymph profile of spiny lobster infested with Octolasmis sp. in bottom cages. The method used was an observation, where 30 lobsters (weight 112.44 ± 24.63 g) were carried out in June–July 2022 at the Pesona Bahari Wongsorejo, Banyuwangi. Hemolymph profile and anatomical pathology data were analyzed, respectively, using the Independent T-Test and Kruskal-Wallis test (α = 95%). The results based on scoring data showed a very significant difference between infected and healthy lobsters (p < 0.01), characterized by the occurrence of tail flakes, lesions on the carapace, gill melanization, and the appearance of ulcers on the ventral abdomen. A very significant change occurred in the hemolymph profile of infected lobsters compared to healthy lobsters (p < 0.01). Total hemocyte count increased with high concentrations of granular cells, respectively 22.1–37.8 ´x 106 cells/mL and 63.75–64.5%, along with lobster damage, while hyaline cells decreased. This study showed that there was a change in anatomic pathology and hemolymph profile between infected and healthy lobsters.
Abdi, R., Setyowati, D. N., & Mukhlis, A. (2022). Pengaruh Penambahan Ekstrak Daun Jeruju (Acanthus ilicifolius) Dengan Dosis Berbeda Pada Pakan Terhadap Sistem Imun Udang Vaname (Litopenaeus vannamei) Yang Diinfeksi Vibrio parahaemolyticus. Jurnal Perikanan, 12(1), 33–44.
Adiyana, K., Supriyono, E., Pamungkas, A., & Thesiana, L. (2015) Evaluasi Penggunaan Sistem Jaring Terhadap Respons Produksi Pendederan Juvenil Lobster Pasir (Panulirus homarus) Menggunakan Teknologi Resirkulasi. Jurnal Kelautan Nasional, 10(3), 139–150.
Amula, N., & Wijaya, D. (2019). Estimasi Potensi dan Tingkat Pemanfaatan Sumber Daya Lobster Pasir (Panulirus homarus) di Perairan Prigi Kabupaten Trenggalek. Jurnal Penelitian Perikanan Indonesia, 25(3): 169–178.
Atherley, N. A. M., Freeman, M. A., & Dennis, M. M. (2020) Post-mortem examination of the Caribbean spiny lobster (Panulirus argus, Latreille 1804) and pathology in a fishery of the Lesser Antilles. Journal of Invertebrate Pathology, 175(107453), 1–14.
Bower, S. M. (2006). Fish diseases and disorders. Canada: Protozoan and Metazoan Infections Parasitic Diseases of Shellfish (CABI), pp: 466–677.
Cerenius, L., & Söderhäll K. (2021). Immune properties of invertebrate phenoloxidases, Developmental & Comparative Immunology, 122, 104098.
Charles, H. M. & Killian, K. A. (2015). Response of the insect immune system to three different immune challenges. Journal of Insect Physiology, 81, 97–108.
Coates, C. J., & Söderhäll, K. (2021). The stress–immunity axis in shellfish. Journal of Invertebrate Pathology, 186, 1–14.
Darwis. (2006). Kajian Parasit Pada Kepiting Bakau (Scylla serrata) Di Wilayah Perairan Bakau Tarakan Kalimantan Timur. [Disertasi]. Sekolah Pasca Sarjana. Universitas Gajah Mada. Hal: 50.
Day, R. D., Fitzgibbon, Q. P., & Gardner, C. (2019). The impact of holding stressors on the immune function and haemolymph biochemistry of Southern Rock Lobsters (Jasus edwardsii). Fish and Shellfish Immunology, 89, 660–671.
Djai, S., Supriyono, E., Nirmala, K., & Adiyana, K. (2017). Respons Total Hemocyte Count Dan Kadar Gukosa Hemolymph Lobster Pasir Panulirus homarus Terhadap Rasio Shelter. Jurnal Ilmu dan Teknologi Kelautan Tropis, 9(1), 125–133.
Ekawati, A. W., Nursyam, H., Widjayanto, E., & Marsoedi. (2012). Diatomae Chaetoceros ceratosporum dalam Formula Pakan Meningkatkan Respon Imun Seluler Udang Windu (Penaeus monodon Fab.). The Journal of Experimental Life Science, 2(1), 20–28.
Fikri, F., Wardhana, D. K., Purnomo, A., Khairani, S., Chhetri, S., & Purnama, M. T. E. (2022). Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia. Veterinary World, 15(7), 1759.
Fikri, F., Purnomo, A., Chhetri, S., & Purnama, M. T. E. (2023). Sea Cucumber-Based Hydroxyapatite-Chitosan Ameliorate Serum Liver Enzymes and Cytokine Levels in Albino Rats with Femoral Bone Defect. Indian Veterinary Journal, 100(7), 23-26.
Gunawan, A. H. (2022). Korelasi parameter kimia air, derajat infestasi dan gejala klinis ektoparasit pada lobster (Panurilus sp.) di keramba perairan laut Trenggalek dan Banyuwangi, Jawa Timur. [Skripsi]. Fakultas Perikanan dan Kelautan. Universitas Airlangga. Hal: 43–56.
Haryanti, Sembiring, S. B. M., Sudewi, Widiastuti, Z., Giri, N. A., & Ketut, S. (2017). Respons Imunitas Benih Lobster, Panulirus homarus dengan Penggunaan Probiotik Pada Pakan Moist. Jurnal Riset Akuakultur, 12(1), 85–97.
Hauton, C. (2012). The scope of the crustacean immune system for disease control. Journal of Invertebrate Pathology, 110(2), 251–260.
Herlinawati, A., Sarjito, & Alfabetian, H. (2017). Infestasi Octolasmis pada kepiting bakau (Scylla serrata) hasil budidaya dari desa Surodadi, Kabupaten Demak, Jawa Tengah. Journal of Aquaculture Management and Technology, 6(4), 11–19.
Hertika, A. M. S., Supriatna, Darmawan, A., Nugroho, B. A., Handoko, A. D., Qurniawatri, A. Y., & Ranita, A. P. (2021). Profil Hemosit Susuh Kura (Sulcospira testudinaria) dalam Rangka Mengevaluasi Kualitas Perairan Wilayah Konservasi Badher Bank, Desa Tawangrejo, Kecamatan Binangun, Kabupaten Blitar. Journal of Fisheries and Marine Research, 5(1), 106–118.
Hidayah, N., Puspita, R., & Mujahidah, M. (2020). Effect of Curcuma domestica Val Extract on Body Weight, Total of Eosinofils and Basofils in Laying Hens Infected with Salmonella pullorum. Jurnal Medik Veteriner, 3(2), 230–235.
Hisyam, M. A. M., Yuniarti, W. M., Theresia, S. W., Yudaniayanti, I. S., Lukiswanto, B. S., Sudjarwo, S. A., & Widiyatno, T. V. (2023). The Efficacy of Madeira Vine Leaf Extract Combined with Turmeric Rhizome Extract Gel Topically on Second-Degree Burn Wound in Albino Rats. Jurnal Medik Veteriner, 6(3), 402–409.
Huang W., Zhang, J., Yang, B., Beerntsen, B.T., Song, H., & Ling E. (2016). DNA duplication is essential for the repair of gastrointestinal perforation in the insect midgut. Scientific Reports, 6(19142), 1–10.
Huong, L. L., Thi, V. L., Thai, T. H., Jones, C., Sang, H. M., Kien, N. T., Bich, N. T. K., Kim, H. P. T., An, K. N., Trung, D. H., Huu, T. P., & Tan, H. D. (2014). Comparative assessment of manufactured pellet feed and traditional trash fish feed on production of tropical rock lobster (Panulirus ornatus) and environmental effects in sea-cage culture in Vietnam. Proceedings of the International Lobster Aquaculture Symposium, pp: 68–81.
Jannah, M., Junaidi, M., Setyowati, D. N., & Azhar, F. (2018). Pengaruh Pemberian Lactobacillus sp. Dengan Dosis Yang Berbeda Terhadap Sistem Imun Udang Vaname (Litopenaeus vannamei) Yang Diinfeksi Bakteri Vibrio parahaemolyticus. Jurnal Kelautan, 11(2), 140–150.
Joseph, F. R. S., & Ravichandran, S. (2012). Shell Diseases of Branchyuran Crabs. Journal of Biological Sciences, 12(3), 117–127.
Kenconojati, H., Ulkhaq, M. F., Azhar, M. H., & Rukmana, N. R. (2023). Vibriocidal Activity of Ethanol Extract of Moringa Leaves and Its Effect on the Growth of Pacific White Shrimp. Jurnal Medik Veterinar, 6(1), 75–81.
Lavilla-Pitogo, C. R., & De La Pena, L. D. (2004). Diseases in Farmed Mud Crabs Scylla spp.: Diagnosis, Prevention, and Control. Philiphines: Aquaculture Department Southeast Asian Fisheries Development Center, pp: 46–48.
Li, H. X., Ma, L. S., Yan, Y., Yang, C. P., & Lin, C. X. (2014). First Records Of The Epizoic Barnacle Octolasmis rullata (Cirripedia: Thoracica: Poecilasmatedae) On The Swimming Crab Portunus sanguinolentus (Decapoda: Portunidae). Biology, 34(1), 76–81.
Liu, M. J., Liu, S., & Liu, H. P. (2021). Recent insights into hematopoiesis in crustaceans. Fish and Shellfish Immunology Reports, 2(100040), 1–10.
Melillo, D., Marino, R., Italiani, P., & Boraschi, D. (2018). Innate Immune Memory in Invertebrate Metazoans: A Critical Appraisal. Frontier in Immunology, 9, 1915.
Nur, Indriyani., & Yusnaini. (2016). Parasitic and Fungal Identification in Bamboo Lobster Panulirus versicolour and Ornate Lobster P. ornatus Cultures. World Academy of Science, Engineering and Technology International Journal of Biotechnology and Bioengineering, 14(12), 1.
Nur, Indriyani, & Yusnaini. (2018). Parasites and histopathology of infected spiny lobster Panulirus spp. cultured in outer of Kendari Bay, Indonesia. Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society, 11(1), 108–117.
Petersen, E. H., Jones, C., & Priyambodo, B. (2014). Bioeconomics of Spiny Lobster Farming in Indonesia. Asian Journal of Agriculture and Development, 10(1), 25–39.
Prastika, D. D., Setiawan, B., Saputro, A. L., Yudaniayanti, I. S., Wibawati, P. A., & Fikri, F. (2020). Effect of Shrimp Chitosan Topically on Collagen Density as Excision Wound Healing Parameter in Albino Rats. Jurnal Medik Veteriner, 3(1), 101–107.
Prastowo, B. W., Penataseputro, T., & Evan, Y. (2022). Necrosis Incidence on The Tail Fin of Sand Lobster (Panulirus homarus) Reared Using Recirculating Aquaculture System (RAS). 11th International and National Seminar on Fisheries and Marine Science:IOP Conf. Series: Earth and Environmental Science, 1118, 1–10.
Ranjan, R., Muktha, M., Ghosh, S., Gopalakrishnan, A., Gopakumar, G., & Joseph, I. (Eds.). (2017). Prioritized Species for Mariculture in India. Kochi: ICAR-CMFRI, pp: 215–220.
Rivaie, A. R., Adiputra, Y. T., Setyawan, A., & Putro, D. H. (2023). Effect of different diets on growth performance, a physiological response, and behavior of spiny lobster Panulirus homarus (Linnaeus, 1758). Jurnal Kelautan Tropis, 26(2), 301–314.
Rokhmani, & Bambang, H. B. (2020). Diversitas Ektoparasit Pada Crustacea Hasil Tangkapan Estuaria Segara Anakan Cilacap Jawa Tengah. Prosiding Seminar Nasional dan Call for Papers, pp: 30–40.
Rostika, R., Ihsan, Y. N., Supriadi, D., Permana, R., Purnama, M. I. C., & Nurfaizi, R. (2021). Control of Ectoparasites Octolasmis sp. in Young Lobsters (Panulirus horamus) using KMNO4 and Herbal Liquid Containing Tamarind Eggplant. International Conference on Fisheries and Aquaculture, Sri Lanka, 19–20 August 2021, pp: 43–45.
Safira, A., Rani, C. A. M., Puspitasari, R. A., Ayuningtyas, A. K. P., Mahendra, Y. A., Purnomo, A., Fikri, F., Chhetri, S., & Purnama, M. T. E. (2022). Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon. Pharmacognosy Journal, 14(4).
Safira, A., Rani, C. A. M., Fikri, F., Purnomo, A., Khairani, S., Chhetri, S., Maslamama, S. T., & Purnama, M. T. E. (2023). Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model. Veterinary World, 16(5).
Saptiani, G., Pebrianto, C. A., Hardi, E. H., & Agustina. (2016). Kerentanan Udang Windu (Panaeus monodon) Terhadap Vibrio harveyi pada Berbagai Stadia Molt dan Osmolaritas. Jurnal Perikanan UGM, 8(1), 19–25.
Sarjito, Haditomo, A. H. C., Desrina, Ferinandika, F. B., Setyaningsih, L., & Prayitno, S. B. (2016). Ectoparasites and Vibrios Associated with Fattening Cultured Mud Crabs [Scylla serrata (Forsskal, 1775)] From Pemalang Coast, Indonesia. Jurnal Sciences and Engineering, 78(4), 207–214.
Shields, J. D. (2011). Diseases of spiny lobsters: A review. Journal of Invertebrate Pathology, 106(1), 79–91.
Silva-Inácio, L. M. da., Machado, G. B. de O., Fortuna, M. D., Sanches, F. H. C., & Costa, T. M. (2016). Infestation by the epibiont Octolasmis lowei in a portunid crab assemblage from a subtropical coast. Nauplius, 24, 1–8.
Smith, V. J., Brown, J. H., & Hauton, C. (2003). Immunostimulation in crustaceans: does it really protect against infection?. Fish & Shellfish Immunology, 15(1), 71–90.
Subhan, R. Y., Supriyono, E., Widanarni, & Daniel, D. (2018). Grow-out of spiny lobster Panulirus sp. with high stocking density in controlled tanks. Jurnal Akuakultur Indonesia, 17(1), 53–60.
Sugumaran, M., & Barek, H. (2016). Critical Analysis of the Melanogenic Pathway in Insects and Higher Animals. International Journal of Molecular Sciences, 17(10), 1–24.
Syahidah, D., & Owens, L. (2020). Histopathology Of Mixed Infections In Redclaw Crayfish (Cherax quadricarinatus) Tissues. Journal of Fisheries and Marine Research, 4(2), 207–213.
Wahjuningrum, D., Effendi, I., Hadiroseyani, Y., Budiardi, T., Diatin, I., Setiawati, M., Hastuti, Y. P., Sudrajat, A. O., Yonvitner, Nuryati, S., & Utami, P. (2022). Health status of spiny lobster Panulirus homarus with sub-mersible net cage system in the different depths at kepulauan seribu, DKI Jakarta. Jurnal Akuakultur Indonesia, 21(1), 68–80.
Wibowo, R. H., Darwis, W., Sipriyadi, Susianti, N., Purbianto, K. A., Gustriana, Rosianti, N., Medani, D. I., & Wulandari, G. D. (2021). Identification of Pathogenic Bacteria on Spiny Lobster (Panulirus homarus) at Quality Control and Fishery Product Safety Agency (BKIPM) of Bengkulu. Advances in Biological Sciences Research, 14, 493–499.
Wu, J., Chen, H., & Huang, D. (2008) Histopathological and Biochemical Evidence of Hepatopancreatic Toxicity Caused Cadmium and Zinc in the White Shrimp, Litopenaeus vannamei. Chemosphere, 73(7), 1019–1026.
Xian, J. Zhang X, Wang D, Li J, Zheng P, Lu Y. (2017).Various cellular responses of different shrimp haemocyte subpopulations to lipopolysaccharide stimulation. Fish & Shellfish Immunology, 69, 195–199.
Yap, F. C., Chen, H. N., & Chan, B. K. K. (2023). Host specificity and adaptive evolution in settlement behaviour of coral-associated barnacle larvae (Cirripedia: Pyrgomatidae). Scientific Reports, 13(9668), 1–13.
Yusgita, L., Kismiyati, Subekti, S., Wulansari, P. D., & Amiin, M. K. (2019). Identification and prevalence of the ectoparasite Octolasmis in sand lobster (Panulirus homarus) and bamboo lobster (Panulirus versicolor) in Floating Net Cages in Sape, Bima Regency, West Nusa Tenggara Province, Indonesia. Proceeding IOP Conf. Series: Earth and Environmental Science 236, 1–6.
Zakwan, M., Sutriana, A., Nurliana, Ammar, M., & Novianti, A. N. (2023). Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test. Jurnal Medik Veteriner, 6(3), 15–19.
Zheng, Z., Li, F., Li, H., Zhu, K., Xu, L., & Yang, F. (2021). Rapid regulation of hemocyte homeostasis in crayfish and its manipulation by viral infection. Fish Shellfish Immunology Reports, 2(100035), 1–9.
Copyright (c) 2024 Sadida Anindya Bahtiar, Gunanti Mahasri, Mohammad Faizal Ulkhaq
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish in this journal agree to the following terms:
1. The journal allows the author to hold the copyright of the article without restrictions;
2. The journal allows the author(s) to retain publishing rights without restrictions;
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA).
11.jpg)
