Primary and Secondary Sexual Characteristics of Kuhli Loach (Pangio kuhlii)
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Kuhli loach (Pangio kuhlii) a popular ornamental freshwater fish species with economic potential, originating from Sumatra, Kalimantan, Java, and Malaysia. The kuhli loach's appeal lies in its vibrant colors and distinctive eel-like body. This study aims to explores the sexual primary and secondary characteristics of the kuhli loach. Conducted at Universitas Airlangga in Banyuwangi, Indonesia, the study involved a thorough analysis of 50 kuhli loaches, evenly split between males and females. Morphological observations revealed significant differences (p < 0.05), such as the elongated body and expanded abdominal region in females, suggesting adaptations for reproduction. Internal examinations, including peritoneal dissections and histological analysis, unveiled distinct stages of spermatogenesis in males and ovarian development in females, providing insights into the reproductive cycle. Meristic and morphometric analyses identified noteworthy differences in pectoral fin rays, suggesting potential roles in reproductive behavior, while other traits exhibited similarity between genders. This study contributes to the understanding of kuhli loach ecology and reproductive biology, highlighting the importance of addressing sustainability concerns arising from the continuous exploitation of wild populations. This study serves as a foundational step towards advancing reproductive improvement in fish and supports the conservation of this unique ornamental fish species, with implications for aquaculture practices and economic considerations.
Acharjee, M. L., & Barat, S. (2014). Loaches of Darjeeling Himalaya and Adjoining areas of West Bengal : their Prospects as Ornamental Fish and Constraints. International Journal of Pure & Applied Bioscience, 2(3), 258–264.
Ajuria Ibarra, H., Kinahan, M., Marcetteau, J., Mehigan, A. J. R., Ziegelmeier, R. O., & Reader, T. (2019). The significance of prey avoidance behavior for the maintenance of a predator color polymorphism. Behavioral Ecology, 30(1), 240–248.
Akter, M., Azom, M., Sabuz, M. R., Islam, M., & Islam, M. (2019). Sexual dimorphism of Canthophrys gongota (Teleostei: Cobitidae) using landmark-based geometric morphometrics in the Atrai river of Bangladesh. Bangladesh Journal of Scientific and Industrial Research, 54(2), 187–194.
Arifin, O. Z., Cahyanti, W., Subagja, J., & Kristanto, H. (2017). Keragaan fenotipe ikan tambakan (Helostoma temminkii, Cuvier 1829) jantan dan betina generasi kedua hasil domestikasi. Media Akuakultur, 12(1), 1–9.
Asiah, N., Junianto, Yustiati, A., & Sukendi. (2018). Morphometric and Meristic of Kalabau fish (Osteochilus melanopleurus) from the Kampar River, Riau Province. Jurnal Perikanan Dan Kelautan, 23(1), 47–56.
Baras, E., Slembrouck, J., Priyadi, A., Satyani, D., Pouyaud, L., & Legendre, M. (2012). Biology and culture of the clown loach Chromobotia macracanthus (Cypriniformes, Cobitidae): 3-Ontogeny, ecological and aquacultural implications. Aquatic Living Resources, 25(2), 119–130.
Budi, D. S., Ardiyan, B. R., Rahmawan, D. B., South, J., Fasya, A. H., & Mukti, A. T. (2024). Gonadal Maturation and Spawning of Barred Loach (Nemacheilus fasciatus) Induced by Topical Gill Hormone Application. Jurnal Medik Veteriner, 7(1), 123–133.
Budi, D. S., Puspitasari, S., Febrianti, R. P. N., Bodur, T., & Mukti, A. T. (2023). A novel technique for mass induction of propagation in small fish species: Hormone immersion. Animal Reproduction Science, 255(113), 107280.
Budi, D. S., Ulkhaq, M. F., & Kenconojati, H. (2020). Increasing of milt production in male silver rasbora (Rasbora argyrotaenia) administered Ovaprim. Indian Veterinary Journal, 97(03), 15–17.
Cahyanti, W., Subagja, J., Kusdiarti, K., Irawan, D., & Arifin, O. Z. (2021). Keragaan bioreproduksi tiga generasi ikan tambakan (Helostoma temminckii Cuvier, 1829). Media Akuakultur, 16(1), 1.
Chumaidi, Priyadi, A., Ginanjar, R., & Sugiarti, L. (2012). Ciri kelamin sekunder pada arwana silver (Sclerophages macrocephalus) varietas pinoh. Jurnal Riset Akuakultur, 7(2), 221.
Endruweit, M. (2016). Range extension of Lepidocephalichthys alkaia (Teleostei: Cobitidae) and notes on its sexual dimorphism. Dong Wu Xue Yan Jiu = Zoological Research, 37(3), 186–188.
Ferdiansyah, F. F., Rahmat, B., & Yuniar, I. (2020). Klasifikasi Dan Pengenalan Objek Ikan Menggunakan Algoritma Support Vector Machine (Svm). Jurnal Informatika Dan Sistem Informasi, 1(2), 522–528.
Fikri, F., Wardhana, D. K., Purnomo, A., Khairani, S., Chhetri, S., & Purnama, M. T. E. (2022). Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia. Veterinary World, 15(7), 1759–1764.
Ganguly, S. (2013). Reproductive Biology in Fishes and Its Diversified. International Journal of Science, Environment and Technology, 2(3), 401–403.
Gao, J., Li, C., Yu, D., Wang, T., Lin, L., Xiao, Y., Wu, P., & Liu, Y. (2023). Comparative Mitogenome Analyses Uncover Mitogenome Features and Phylogenetic Implications of the Parrotfishes (Perciformes: Scaridae). Biology, 12(3), 1–19.
Greven, H. (2019). Gonads, genitals, and reproductive biology. In J. P. Evans, A. Pilastro, & I. Schlupp (Eds.), Ecology and Evolution of Poeciliid Fishes. University of Chicago Press. pp: 3–17.
Hidayat, R., Carman, O., & Alimuddin, A. (2024). Early Sex Differentiation of Climbing Perch (Anabas testudineus Bloch.): A Pathway to Feminization. Jurnal Medik Veteriner, 7(1), 143–154.
Johnston, M. E., Kelly, J. T., Lindvall, M. E., McElreath, R., & Klimley, A. P. (2017). Experimental evaluation of the use of vision and barbels as references for rheotaxis in green sturgeon. Journal of Experimental Marine Biology and Ecology, 496(July), 9–15.
Kerfoot, J. R., & Schaefer, J. F. (2006). Ecomorphology and habitat utilization of Cottus species. Environmental Biology of Fishes, 76(1), 1–13.
Manoharan, S., Kuppu, R., & Uthandakalaipandian, R. (2019). Deciphering the morphological and molecular characteristics of fresh water fish Lepidocephalus thermalis (V.)-DNA barcode approach. Indian Journal of Experimental Biology, 57(August), 573–579.
Passos, C., Tassino, B., Rosenthal, G. G., & Reichard, M. (2015). Reproductive behavior and sexual selection in annual fishes. In Annual Fishes: Life History Strategy, Diversity, and Evolution. CRC Press. pp: 207–229.
Prakoso, V. A., Subagja, J., & Kristanto, A. H. (2017). Aspect of reproductive biology and growth pattern of barred loach (Nemacheilus fasciatus) reared in aquarium. Media Akuakultur, 12(2), 67–74.
Priyadi, A., Permana, A., Kusrini, E., Hayuningtyas, E. P., & Nur, B. (2024). Captive breeding of endangered betta fish, Betta rubra, under laboratory conditions. Fisheries and Aquatic Sciences, 27(4), 213–224.
Radkhah, A. R., Eagderi, S., & Nahavandi, R. (2021). A study on the biological characteristics of kuhli loach (Pangio kuhlii Valenciennes 1846) as an ornamental fish species. Iranian of Ornamental Aquatics, 8(3), 1–8.
Sabrina, A. N., Mukti, A. T., Suciyono, Kenconojati, H., Ulkhaq, M. F., Fasya, A. H., Lamadi, A., Imlani, A., & Mariah, S. R. (2023). Color brightness and growth levels of goldfish (Carassius auratus) reared with different light spectrums. Jurnal Medik Veteriner, 6(2), 250–255.
Sharma, N. K., Mir, J. I., Pandey, N., & Singh, R. (2014). Morphometric and meristic characteristics of birdi loach, Botia birdi (Chaudhuri, 1909) from a Tributary of Indus Basin, Jammu and Kashmir, India. World Journal of Fish and Marine Sciences, 6(3), 262–266.
Snake, M., Maluwa, A., Zidana, H., Chigwechokha, P., & Simwaka, M. (2020). Production of a predominantly male tilapia progeny using two Malawian tilapias, Oreochromis shiranus and Oreochromis karongae. Aquaculture Reports, 16(January), 100274.
Syafariyah, N. K., Sulmartiwi, L., & Budi, D. S. (2023). Incubation temperature effects on some hatching parameters of silver rasbora (Rasbora argyrotaenia) egg. Journal of Applied Aquaculture, 31(1), 16–26.
Tahir, N. D. M., Matori, M. F., & Gan, H. M. (2024). First Report of Aeromonas schubertii Infection in Striped Snakehead Channa striata (Bloch, 1793) Fingerlings in Malaysia. Jurnal Medik Veteriner, 7(1), 33–40.
Taugbøl, A., Quinn, T. P., Østbye, K., & Asbjørn Vøllestad, L. (2020). Allometric relationships in morphological traits associated with foraging, swimming ability, and predator defense reveal adaptations toward brackish and freshwater environments in the threespine stickleback. Ecology and Evolution, 10(23), 13412–13426.
Tyler, C. R., Sumpter, J. P., & Witthames, P. R. (1990). The dynamic of oocyte growth during vitellogenesis in rainbow trout (Oncorhynchus mykiss). Biology of Reproduction, 43, 202–209.
Ulkhaq, M. F., Abdillah, A. A., Nindarwi, D. D., Kenconojati, H., Budi, D. S., Suciyono, & Santanumurti, M. B. (2019). Identification and morphological characteristic of ornamental fish in Wongsorejo Beach, Banyuwangi, East Java, Indonesia. Indian Veterinary Journal, 96(11).
Urban, S., Gerwin, J., Hulsey, C. D., Meyer, A., & Kratochwil, C. F. (2022). The repeated evolution of stripe patterns is correlated with body morphology in the adaptive radiations of East African cichlid fishes. Ecology and Evolution, 12(2), 1–11.
Uribe, M. C., Grier, H. J., & Mejía-Roa, V. (2014). Comparative testicular structure and spermatogenesis in bony fishes. Spermatogenesis, 4(3), e983400.
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