Mouli Edward, Henry Dominica, Ferdiansyah Mahyudin, Fedik Abdul Rantam

= http://dx.doi.org/10.20473/joints.v9i2.2020.34-54
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Background: Bone defects to date have been a significant problem in the Orthopedics field. Hydroxyapatite is a bone graft that is often chosen if it has osteoconductive properties. Platelet-rich plasma (PRP) has a higher platelet concentration than the concentration in normal blood, capable of providing many bioactive molecules in physiological proportions. Hydroxyapatite given freeze-dried PRP is expected to create a graft that can strengthen the matrix while promoting osteoinduction.

Methods: This study compares the effects of regeneration on the bone between bovine hydroxyapatite (BHA) and bovine hydroxyapatite with freeze-dried platelet-rich plasma (FD-PRP) as a bone graft in bone defect of the femoral white rabbit. The 12 equal New Zealand white rabbits aged 6-9 months are divided into two groups. Bone defects were made in the lower femoral meta-diaphysis with a diameter of 2.5 mm. The defects were filled with BHA with FD-PRP allograft in the treatment group and BHA in the control group. Both groups will be sacrificed in the third and sixth weeks, then evaluated histologically for microvascular structure, osteoblasts, woven bone, type-I collagen, osteocalcin, alkaline phosphatase, and immunoglobulin G.

Results: During the evaluation in week 3 and 6, microvascular structure, osteoblast, and type-I collagen decreased in both groups with insignificant differences (p>0.05). Woven bone, osteocalcin, and immunoglobulin G increased in the treatment group but was not significant (p>0.05). Alkaline phosphatase increased higher in the treatment group, with a considerable difference in the sixth week (p=0.008).

Conclusion: The elevation in the production of woven bone, osteocalcin, and alkaline phosphatase at the third and sixth-week evaluations highlight the possibility that administering BHA given FD-PRP may have contributed to the healing of bone defects.


Alkaline phosphatase; Bone graft; Bovine hydroxyapatite; Freeze dried platelet rich plasma; Osteocalcin; Woven bone

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Brydone AS, Meek D, MacLaine S. Bone grafting, orthopaedic biomaterials, and the clinical need for bone engineering. Proc Inst Mech Eng Part H J Eng Med. 2010;224(12):1329–43.

Nandi SK, Roy S, Mukherjee P, Kundu B, De DK, Basu D. Orthopaedic applications of bone graft & graft substitutes: A review. Indian J Med Res. 2010;132(7):15–30.

Myeroff C, Archdeacon M. Autogenous bone graft: Donor sites and techniques. J Bone Jt Surg - Ser A. 2011;93(23):2227–36.

Gleeson JP, Plunkett NA, O’Brien FJ. Addition of hydroxyapatite improves stiffness, interconnectivity and osteogenic potential of a highly porous collagen-based scaffold for bone tissue regeneration. Eur Cell Mater. 2010;20(218):30.

Ngoc N. Basic Knowledge of Bone Grafting. Bone Grafting. 2012;

Venkatesan J, Kim S-K. Nano-hydroxyapatite composite biomaterials for bone tissue engineering—a review. J Biomed Nanotechnol. 2014;10(10):3124–40.

Rivera-muñoz EM. Hydroxyapatite-Based Materials : Synthesis and Characterization. 2000;

Mahyudin F, Rantam FA. Regenerasi pada Massive Bone Defect dengan Bovine Hydroxyapatite sebagai Scaffold Mesenchymal Stem Cell. J Biosains Pascasarj. 2011;13(3):179–95.

Sutherland D, Bostrom M. Grafts and bone graft substitutes. In: Bone Regeneration and Repair. Springer; 2005. p. 133–56.

Kloping YP, Desnantyo AT, Rehatta NM. The effects of Platelet-Rich-Plasma (PRP) injection on ligament injury. Bali Med J. 2016;5(1):36–42.

Kasten P, Vogel J, Geiger F, Niemeyer P, Luginbühl R, Szalay K. The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials. 2008;29(29):3983–92.

Nakatani Y, Agata H, Sumita Y, Koga T, Asahina I. Efficacy of freeze-dried platelet-rich plasma in bone engineering. Arch Oral Biol. 2017;73:172–8.

Rachmawati T, Astuti SP. The Effect of Allogenic Freeze Dried Platelet- Rich Plasma in Immunological Responses of Rabbits. J Stem Cell Res Tissue Eng. 2017;1(1):325–7.

Jensen SS, Terheyden H. Bone augmentation procedures in localized defects in the alveolar ridge: clinical results with different bone grafts and bone-substitute materials. In: Database of Abstracts of Reviews of Effects (DARE): Quality-Assessed Reviews [Internet]. Centre for Reviews and Dissemination (UK); 2009.

Devescovi V, Leonardi E, Ciapetti G, Cenni E. Growth factors in bone repair. Chir Organi Mov. 2008;92(3):161–8.

Herrmann M, Klitscher D, Georg T, Frank J, Marzi I, Herrmann W. Different kinetics of bone markers in normal and delayed fracture healing of long bones. Clin Chem. 2002;48(12):2263–6.

Wesseling-Perry K, Salusky IB. Chronic kidney disease: mineral and bone disorder in children. In: Seminars in nephrology. Elsevier; 2013. p. 169–79.

Marchelli D, Piodi LP, Corradini C, Parravicini L, Verdoia C, Ulivieri FM. Increased serum OPG in atrophic nonunion shaft fractures. J Orthop Traumatol. 2009;10(2):55–8.

Moghaddam A, Müller U, Roth HJ, Wentzensen A, Grützner PA, Zimmermann G. TRACP 5b and CTX as osteological markers of delayed fracture healing. Injury. 2011;42(8):758–64.

Jacobsen G, Easter D. Allograft vs. xenograft: Practical considerations for biologic scaffolds. Musculoskelet Transpl Found. 2008;

Dumitrescu AL. Bone grafts and bone graft substitutes in periodontal therapy. In: Chemicals in surgical periodontal therapy. 2011.


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