The Osteogenic Capacity of HumanAmniotic Membrane Mesenchymal Stem Cell (hAMSC) and Potential for Application in Maxillofacial Bone Reconstruction in Vitro Study
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Amniotic membrane of human placenta is a source of abundant mesenchymal stem cell (hAMSC) which makes it a potential source of allogeneic multipotent cell for bone healing. However, much has to be explored about its isolation procedure and the osteogenic differentiation potential. The aims of this study are to establish the procurement procedure of human amniotic membrane, the isolation and culture of hAMSC, the MSC phenotypic characterization, and the in vitro osteogenic differentiation of hAMSC. Results of the study are as follows. The quality of human amniotic membrane would be best if procured from Caesarean operation under highly aseptic condition to avoid fungal and bacterial contamination on the culture. Isolation procedure using modified Soncini protocol yielded large amount of MSC with high proliferative capacity in culture medium. Characterization of hAMSC showed that the majority of the target cells exhibited specific MSC markers (CD10S and CD90) with a small number of these cells expressing CD45the marker of hematopoeitic cells. The in vitro osteogenic differentiation of hAMSC followed by Alizarin Red staining showed that osteoblastic differentiation was detected in a significantly high number of cells. This study concludes that hAMSCs isolated from human amniotic membrane have the capacity for in vitro osteogenesis which makes them be one of the potential allogeneic stem cells for application in maxillofacial bone reconstruction.
Caplan, A.I. (2007) Fundamental of Stem Cells Tissue Engineering. In: Fisher, J.P., Mikos, A.G. and Bronzino, J.D., Eds., Tissue Engineering, CRC Press, Boca Raton, 1-1, 1-9.
Diaz-Prado, S., Lopez, E.M., Gomez, T.H., Vazquez, E.R., Boquete, I.F., de Toro, F.J. and Blanco, F.J. (2011) Isolation and Characterization of Mesenchymal Stem Cells from Human Amniotic Membrane. Tissue Engineering Part C, 17, 49-59. http:/'dx.doi.org,I0.1089/ten.tec.201 0.0136
Gregory, C.A., Gunn, W.G., Peister, A. and Prockop, D.J. (2005) An Alizarin Redbased Assay of Mineralization by Adherent Cells in Culture: Comparison with Cetylpyridinium Chloride Extraction. Analytical Biochemistry, 329, 77-84. http://dx.doi.org/l0.106/j.ab.2004.02. 002
Ilancheran, S., Moodley, Y. and Manuelpillai, U (2009) Human Fetal Membranes: A Source of Stem Cells for Tissue Regeneration and Repair? Placenta, 30, 2-10. http:.'/dx.doi.org I0.1016 'placenta.2008.09.009
Jiang, Y., Jahagirdar, B.N. and Reinhardt, L.R. (2002) Pluripotency of Mesenchymal Stem Cells Derived from Adult Marrow. Nature,418, 41-49. http:/'dx.doi.oreI0.1126/science.284. 5411.143
Kretlow, J.D., Yu, Q.J., Wei, L., Wen, J.Z., Tan, H.H., Zhou, G.D., Baggett, L.S., Mikos, A.G. and Cao, Y.L. (2008) Donor Age and Cell Passage Affects Differentiation Potential of Murine Bone Marrow-Derived Stem Cells. BMC Cell Biology, 9, 60. http: '/dX .doi.onul0.1186/1471-2121-9-60
Kubo, M. Sonoda, Y., Muramatsu, R. and Usui, M. (2001) Immunogenicity of Human Amniotic Membrane in Experimental Xenotransplantation. Investigative Ophthalmology & Visual Science, 42, 1539-1546.
Mild, T., Lehmann, T., Cai, H., Stolz, D.B. and Strom, S.C. (2005) Stem Cell Characteristics of Amniotic Epithelial Cells. Stem Cells, 23, 1549-1559. http://dx.doi.org/l0.1634.stemcells.2 004-0357
Pittenger, M.F., MacKay, A.M., Beck, S.C., et al. (1999) Multilineage Potential of Adult Human Mesenchymal Stem Cells. Science,284, 143-147. http:'/dx.doi.org/l0.11261science.284 .5411.143
Randall, M.W. (2004) Bony Reconstruction of the Jaws. In: Miloro, M., Ed., Peterson's Principles of Oral and Maxillofacial Surgery, 2nd Edition, BC Decker Inc., London, 783.
Sakaguchi, Y.,Sekiya, I., Yagishita, K. and Muneta, T. (2005) Comparison of Human Stem Cells Derived from Various Mesenchymal Tissues. Superiority of synovium as a cell source. Arthritis & Rheumatism, 52, 25212529.http://dx.doi.om,I0.1002/a n.21212
Shuang, Z.H., Ping, S. and Xi, N.P.(2010) Culture and Identification of Human Amniotic Mesenchymal Stem Cells. Original article. Chinese Medical Sciences Joumal., 25, 211-214. http://dx.doi.orn.I0.1016/S100l9294( 11)60004-7.
Solomon, A., Rosenblatt, M., Monroy, D., Ji, Z., Pflugfelder, S.C., Tseng, S.C.G. (2001) Suppression of Interleukin I (Alpha) and Interleukin 1 (Beta) in Human Limbal Epithelial Cells Cultured on the Amniotic Membrane Stromal Matrix. British Journal of Ophthalmology, 85, 444-449. http://dx.doi.org/l0.1136 bjo.85.4.444
Toda, A., Okabe, M., Yoshida, T. and Nikaido, T. (2007) The Potential of Amniotic Membrane/Amnion-Derived Cells for Regeneration of Various Tissues. Critical review. Journal of Pharmacological Sciences, 105, 215- 228.http://dx.doi.org/J0.1254jphs.CR 0070034
Tseng,S.C.G., Li, D.Q. and Ma, X. (1999) Suppression of Transforming Growth actor-Beta Isoforms, TGF- Receptor Type II, and Myofibroblast Differentiation in Cultured Human Corneal and Limbal Fibroblasts by Amniotic Membrane Matrix. Journal of Cellular Physiology,179, 325-335. http: dx.doi.org/10.1186/1471-2121-9- 60
Wang, M., Yoshida, A., Kawashima, H., Ishizaki, M., Takahashi, H. and Hori, (2006) Immunogenicity and Antigenicity of Allogeneic Amniotic Epithelial Transplants Grafted to the Cornea Conjunctiva, and Anterior Chamber Investigative Ophthalmology & Visual Science, 47, 15221532.http://dx.doi.org/I0.1167/io vs.05-0787
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