In Vitro Antibacterial Activity of Averrhoa bilimbi Leaves Ethanol Extract Against Salmonella typhi
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Highlights:
1. Typhoid fever, a major health problem for developing countries, requires alternative therapy to reduce drug resistance.
2. A. bilimbi, a native plant of Indonesia, has antibacterial potential, which is effective against S. typhi in vitro.
Abstract
Introduction: Some native plants in Indonesia have potential effects on health and medication. This study aimed to determine the potential antibacterial effect of Averrhoa bilimbi (A. bilimbi) leaves ethanol extract against Salmonella typhi (S. typhi) bacteria.
Methods: This was an experimental study. The antibacterial potency of A. bilimbi leaves was measured against S. typhi in vitro. Different concentrations of the leaves' ethanol extract were prepared. The minimum inhibitory concentration (MIC) was evaluated by a macro-dilution method using Mueller Hinton broth. The minimum bactericidal concentration (MBC) value was observed by subculturing the specimen from the previous dilution tube to the nutrient agar.
Results: The MIC of A. bilimbi leaves ethanol extract against S. typhi bacteria could not be determined because all the treatment tubes' colors were dark and turbid. Meanwhile, the MBC value was at 500 mg/ml.
Conclusion: A. bilimbi leaves extract had potential bactericidal effects against S. typhi with a MIC value that could not be determined.
Mogasale V, Maskery B, Ochiai RL, et al. Burden of typhoid fever in low-income and middle-income countries: a systematic, literature-based update with risk-factor adjustment. Lancet Glob Heal 2014; 2: e570–e580.
Anak AALP, Putu IB, Putu AS, et al. Identification of Pathogenic Bacteria in Food Samples from Cafeterias of A University in Denpasar, Indonesia. Folia Medica Indonesiana 2022; 58: 313–317.
Saporito L, Titone L. Typhoid fever. International Encyclopedia of Public Health (Second Edition) 2016; 277–283.
Alba S, Bakker MI, Hatta M, et al. Risk Factors of Typhoid Infection in the Indonesian Archipelago. PLoS One 2016; 11: e0155286.
Carroll, et al. Jawetz, Melnick, & Ade/berg's Medical Microbiology 21st edition. 21st ed. 2016.
Das S, Samajpati S, Ray U, et al. Antimicrobial resistance and molecular subtypes of Salmonella enterica serovar Typhi isolates from Kolkata, India over a 15 years period 1998–2012. Int J Med Microbiol 2017; 307: 28–36.
Cuypers WL, Jacobs J, Wong V, et al. Fluoroquinolone resistance in Salmonella: insights by whole-genome sequencing. Microb Genomics; 4. Epub ahead of print 1 July 2018. DOI: 10.1099/mgen.0.000195.
Alhassan A, Ahmed Q. Averrhoa bilimbi Linn.: A review of its ethnomedicinal uses, phytochemistry, and pharmacology. J Pharm Bioallied Sci 2016; 8: 265.
Bijoy K, Mohammed I, Ayeasha M, et al. Preliminary Antimicrobial, Cytotoxic and Chemical Investigations of Averrhoa bilimbi Linn. and Zizyphus mauritiana Lam. Bangladesh Pharm J 2014; 14: 127–131.
Setyawan HY, Sukardi S, Nareswari BF. The phytochemical potential of Averrhoa bilimbi – A review. IOP Conf Ser Earth Environ Sci 2021; 733: 012091.
Anitha R, Geetha R, Lakshmi T. Averrhoa bilimbi Linn'Nature's Drug Store- A Pharmacological Review. Int J Drug Dev Res 2011; 3: 101–106.
Aziz MA, Shahariar R, Mahfuzul I, et al. A Comparative Study on Antibacterial Activities and Cytotoxic Properteis of Various Leaves Extracts of Averrhoa Bilimbi. Int J Pharm Sci Res 2014; 5: 913–918.
Iwansyah AC, Desnilasari D, Agustina W, et al. Evaluation on the physicochemical properties and mineral contents of Averrhoa bilimbi L. leaves dried extract and its antioxidant and antibacterial capacities. Food Sci Technol 2021; 41: 987–992.
Yan Y, Li X, Zhang C, et al. Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review. Antibiotics 2021; 10: 318.
Safitri I, Leliqia NP. A Review of Phytochemical Properties, Antibacterial Activity, And Toxicity Study of Averrhoa Bilimbi Leaves And Fruit. J Pharm Sci Appl 2021; 3: 32–39.
Nurzaman F, Djajadisastra J, Elya B. Identifikasi Kandungan Saponin dalam Ekstrak Kamboja Merah (Plumeria rubra L.) dan Daya Surfaktan dalam Sediaan Kosmetik. J Kefarmasian Indones 2018; 85–93.
Widhianto EK, Elmarda RV, Rakhmawatie MD. Effectivity in Vitro of Averrhoa bilimbi L Ethanolic Extract Against Escherichia coli and Staphylococcus aureus Growth. Proceeding Int Semin Occup Heal Med Sci 2017; 140–148.
Kaczmarek B. Tannic Acid with Antiviral and Antibacterial Activity as A Promising Component of Biomaterials”A Minireview. Materials (Basel) 2020; 13: 3224.
Yuan G, Guan Y, Yi H, et al. Antibacterial Activity and Mechanism of Plant Flavonoids to Gram-Positive Bacteria Predicted from Their Lipophilicities. Sci Rep 2021; 11: 10471.
Hasdiana F, Kuswarini S, Koendhari E. Antibacterial Activity of Belimbing Wuluh Extract on Salmonella typhi Growth. Folia Medica Indonesiana 2012; 48: 144–149.
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