Efek Preventif Isolat Kasein Yoghurt Susu Kambing Terhadap Kadar Malondialdehida (MDA) Tikus Putih (Rattus Norvegicus) dan Histopatologi Lambung yang Dipapar 2,3,7,8-Tetrachloro-Dibenzo-P-Dioxin (TCDD)
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Dioksin merupakan senyawa toksik yang termasuk dalam senyawa organoklorin bersifat sangat reaktif dan menghasilkan radikal bebas yang dapat merusak sel lambung. 2,3,7,8-Tetrachlorinedibenzo-p-dioksin (TCDD) merupakan jenis dioksin yang memiliki toksisitas paling tinggi. Penelitian ini bertujuan mengetahui efek preventif pemberian yoghurt susu kambing terhadap paparan dioksin yang diamati kadar malondialdehida (MDA) dan gambaran histopatologi lambung. Penelitian bersifat eksperimental menggunakan Rancangan Acak Lengkap (RAL). Tikus putih (Rattus norvegicus) dibagi 6 kelompok perlakuan yaitu kelompok normal, kontrol kasein, kontrol positif, perlakuan 1, perlakuan 2, dan perlakuan 3. Kadar MDA lambung diukur menggunakan metode uji Thiobarbituric Acid (TBA) dengan pengukuran spektrofotometri UV-Vis panjang gelombang 530 nm dan gambaran histopatologi lambung menggunakan pewarnaan Hematoksilin Eosin (HE) diamati berupa kerusakan sel mukosa lambung. Kadar MDA lambung dianalisismenggunakan uji statistik one way ANOVA, α=5% dan analisis histopatologi lambung diolah secara deskriptif. Hasil penelitian menunjukkan kasein yoghurt susu kambing dengan dosis 600 dan 900 mg/kgBB merupakan dosis terbaik dalam mencegah kenaikan kadar MDA lambung dari tikus putih (Rattus norvegicus) terpapar TCDD secara signifikan (p<0,01), dan dosis 900mg/kgBB mencegah terjadi erosi sel mukosa lambung. Kesimpulan penelitian yaitu pemberian kasein yoghurt susu kambing mampu mencegah kenaikan kadar MDA dan terjadi erosi sel mukosa lambung akibat TCDD.
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