The success rate of intrauterine insemination in sperm preparation swim-up method at room temperature compared to the incubator temperature
Downloads
HIGHLIGHTS
- There were no significant differences in TMSC, sperm motility, sperm morphology, and DFI in sperm preparation using the swim-up method at 27°C and 37°C. However, this study provided an overview of the average improvement of DFI at 27°C compared to 37°C.
- There was no significant difference in the pregnancy rate of IUI in sperm preparation using the swim-up method at 27°C and 37°C.
ABSTRACT
Objective: This study aimed to determine the effect of temperature during sperm preparation on total sperm motile count (TMSC), sperm motility, sperm morphology, DNA fragmentation index (DFI), and pregnancy rate.
Materials and Methods: A quasi-experimental laboratory study with pre- and post-test control group was conducted at Sekar Fertility Clinic, Dr. Moewardi General Hospital, Surakarta, Indonesia. A total of 20 sperm samples from infertile patients were prepared using the swim-up method at 27°C (group 1) and 37°C (group 2). TMSC, motility, morphology, and DFI examinations were performed. In addition, IUI was performed to confirm pregnancy rate. Sperm DNA fragmentation was determined using Sperm Chromatin Dispersion/SpermFunc DNAf test. Sperm DNA fragmentation was characterized by a halo <30% of the volume of the sperm head.
Results: Group 1 had mean TMSC of 13.77 ± 9.30, while group 2 had 14.82 ± 8.82; p=0.218. Group 1 had a motility value 82.25+12.77 and group 2 had 82.55 ± 11.69; p=0.968. The morphological value for group 1 was 11.25 ± 5.15 and group 2 was 11.6 ± 5.34; p=0.626. The mean DFI for group 1 was 17.79 ± 10.88 and group 2 was 18.18 ± 12.95; p=0.765. Pregnancy rate in group 1 was 10% and group 2 was 20%; p=1.000.
Conclusion: There were no significant differences in TMSC, sperm motility, sperm morphology, DFI, and pregnancy rate in sperm preparation using the swim-up method at 27°C and 37°C.
Agarwal A, Mulgund A, Hamada A, Chyatte MR. A unique view on male infertility around the globe. Reprod Biol Endocrinol. 2015;13(1):1–9.
Jo J, Kang MJ. Successful treatment of oligoasthenozoospermia using traditional Korean medicine resulting in spontaneous pregnancy: Two case reports. Explor J Sci Heal. 2016;12(2):136–8.
Jerng UM, Jo JY, Lee S, Lee JM, Kwon O. The effectiveness and safety of acupuncture for poor semen quality in infertile males: A systematic review and meta-analysis. Asian J Androl. 2014;16(6):884–91.
Ombelet W. Evidence-based recommendations for IUI in daily practice. Middle East Fertil Soc J. 2013;18(2):74–7.
Schlegel PN, Sigman M, Collura B, De Jonge CJ, Eisenberg ML, Lamb DJ, et al. Diagnosis and treatment of infertility in men: AUA/ASRM guideline part II. Fertil Steril. 2021;115(1):62–9.
Ombelet W, Dhont N, Thijssen A, Bosmans E, Kruger T. Semen quality and prediction of IUI success in male subfertility: A systematic review. Reprod Biomed Online. 2014;28(3):300–9.
Thijssen A, Klerkx E, Huyser C, Bosmans E, Campo R, Ombelet W. Influence of temperature and sperm preparation on the quality of spermatozoa. Reprod Biomed Online. 2014;28(4):436–42.
Sugihara A, Van Avermaete F, Roelant E, Punjabi U, De Neubourg D. The role of sperm DNA fragmentation testing in predicting intra-uterine insemination outcome: A systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol. 2020;244:8–15.
Schwarz C, Köster M, van der Ven K, Montag markus. Temperature-induced sperm nuclear vacuolisation is dependent on sperm preparation. Andrologia. 2012;44(SUPPL.1):126–9.
Zhou Y, Meng T, Wu L, Duan Y, Li G, Shi C, et al. Association between ambient temperature and semen quality: A longitudinal study of 10 802 men in China. Environ Int. 2020;135(December 2019).
Lestari SW, Sari T, Pujianto DA. Sperm DNA fragmentation and apoptosis levels: A comparison of the swim up and the density gradient centrifugation methods for sperm preparation. Online J Biol Sci. 2016;16(4):152–8.
Agarwal A, Sharma R, Beydola T. Sperm Preparation and Selection Techniques. Med Surg Manag Male Infertil. 2014;244–244.
Ayala C, Steinbeger E, Smith D. The influence of semen analysis parameters on the fertility potential of infertile couples. J Androl. 1996;718–25.
Cao XW, Lin K, Li CY, Yuan CW. [A review of WHO Laboratory Manual for the Examination and Processing of Human Semen (5th edition)]. Zhonghua Nan Ke Xue. 2011;17(12):1059–63.
Le MT, Nguyen TAT, Nguyen HTT, Nguyen TTT, Nguyen VT, Le DD, et al. Does sperm DNA fragmentation correlate with semen parameters? Reprod Med Biol. 2019;18(4):390–6.
WHO. WHO Laboratory Manual for the Examination and Processing of Human Semen. World Health. 2010.
Will MA, Clark NA, Swain JE. Biological pH buffers in IVF: Help or hindrance to success. J Assist Reprod Genet. 2011;28(8):711–24.
Muthigi A, Jahandideh S, Bishop LA, Naeemi FK, Shipley SK, O'Brien JE, et al. Clarifying the relationship between total motile sperm counts and intrauterine insemination pregnancy rates. Fertil Steril. 2021;115(6):1454–60.
Zini A, Libman J. Sperm DNA damage: Clinical significance in the era of assisted reproduction. Cmaj. 2006;175(5):495–500.
Ramaraju GA, Teppala S, Prathigudupu K, Kalagara M, Thota S, Kota M, et al. Association between obesity and sperm quality. Andrologia. 2018;50(3):1–12.
Durairajanayagam D. Lifestyle causes of male infertility. Arab J Urol. 2018;16(1):10–20.
Ménézo Y, Dale B, Cohen M. DNA damage and repair in human oocytes and embryos: A review. Zygote. 2010;18(4):357–65.
Harlev A, Agarwal A, Gunes SO, Shetty A, du Plessis SS. Smoking and Male Infertility: An Evidence-Based Review. World J Mens Health. 2015;33(3):143.
Agarwal A, Durairajanayagam D, du Plessis SS. Utility of antioxidants during assisted reproductive techniques: An evidence based review. Reprod Biol Endocrinol. 2014;12(1):1–19.
WHO. WHO Laboratory Manual for the Examination and Processing of Human Semen. World Health. 2010;fifth edit.
Li R, Liu Y, Pedersen HS, Callesen H. Effect of ambient light exposure of media and embryos on development and quality of porcine parthenogenetically activated embryos. Zygote. 2014;23(3):378–83.
Ahmed B, Vaidyanathan G, Arumughan Pillai S, AlSabti J, Khaduri M Al, Pathare A. Factors Influencing the Success Rate of Intrauterine Insemination: ARetrospective Study in Sultan Qaboos University Hospital. J Women's Heal Care. 2017;06(05).
Yavuz A, Demirci O, Sözen H, Uludoǧan M. Predictive factors influencing pregnancy rates after intrauterine insemination. Iran J Reprod Med. 2013;11(3):227–34.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1. Copyright of the article is transferred to the journal, by the knowledge of the author, whilst the moral right of the publication belongs to the author.
2. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution-Non Commercial-Share alike (CC BY-NC-SA), (https://creativecommons.org/licenses/by-nc-sa/4.0/)
3. The articles published in the journal are open access and can be used for non-commercial purposes. Other than the aims mentioned above, the editorial board is not responsible for copyright violation
The manuscript authentic and copyright statement submission can be downloaded ON THIS FORM.
