The potentiation of Mangifera casturi bark extract on interleukin- 1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction

Bayu Indra Sukmana, Theresia Indah Budhy, I Gusti Aju Wahju Ardani

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Background: The main oral health problem in Indonesia is the high number of tooth decay. Tooth extraction is the treatment often received by patients who experience tooth decay and the wound caused by alveolar bone resorption. Bark of Mangifera casturi has been studied and proven to contain secondary metabolite which has the ability to increase osteoblast’s activity and suppress osteoclast’s activity. Purpose: The purpose of this study was to analyze interleukin-1 beta (IL-1β) and bone morphogenic protein-2 (BMP-2) activities during bone remodeling after Mangifera casturi’s bark extract treatment. Method: This study was laboratory experimental research with randomized post-test only control group design. The Mangifera casturi bark was extracted using 96% ethanol maceration and n-hexane fractionation. This study used 40 male Wistar rats which are divided into 4 groups and the tooth extraction was performed on the rats’ right mandible incisive tooth. The four groups consisted of 6.35%, 12.7%, 25.4% extract treatment group, and a control group. Wistar’s mandibles were decapitated on the 7th and 14th day after extraction. Antibody staining on preparations for the examination of IL-1β and BMP-2 expressions was done using immunohistochemistry. Result: There was a significant difference of IL-1β and BMP-2 expressions in 6,35%, 12,7%, and 25,4% treatment groups compared to control group with p<0.05. Conclusion: Mangifera casturi’s bark extract was able to suppress the IL-1β expression and increase the BMP-2 expression during bone remodeling after tooth extraction.


bone morphogenic protein-2; bone remodeling; interleukin-1β; Mangifera casturi (Kosterm.); tooth extraction

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Fogelman I, Gnanasegaran G, Van der Wall H. Radionuclide and hybrid bone imaging. Berlin, Germany: Springer; 2012. p.29-30

Balaji SM. Textbook of oral and maxillofacial surgery. Philadelphia, USA: Elsevier; 2007. p.985.

Ghodke MH, Bhoyar SC, Shah SV. Prevalence of mandibular fractures reported at CSMSS dental collegem aurangabad from February 2008 to September 2009. J Int Soc Prevent Communit Dent 2013; 3(2): 51-8.

Rusnawati M, Carnozzi M, Moroni E, Boltazzi B, Peri G, Indraccolo S, Anadoni A, Mantovani A, Presta M. Selective recognition of fibroblast, growth factor 2 by the long pentraxin PTX-3 inhibits angiogenesis. American society of Haematology 2004; 104(1): 92-101.

Dimitriou R, Tsiridis E, Giannoudis PV. Current concepts of molecular aspects of bone healing. Int J Care Injured 2005; 36(12): 1392–404.

Belibasakis GN, Bostanci N. The RANKL-OPG system in clinical periodontology. J Clin Periodontol 2012; 39(3): 239-48.

Oryan A, Alidadi S, Moshiri A, Maffulli N. Bone regenerative medicine: classic options, novel strategies, and future directions. Journal of Orthopedic Surgery and Research 2014; 9: 18.

Suhartonoa E, Viani E, Rahmadhan MA, Gultom IS, Rakhman MF, Indrawardhana D. Total flavonoid and antioxidant activity of some selected plants in South Kalimantan of Indonesia. AsiaPacific Chemical, Biological & Environmental Engineering Society Procedia 2012; 4: 235-9.

Hapidin H, Abdullah H, Soelaiman IN. The potential role of quercus infectoria gall extract on osteoblast function and bone metabolism. Open Journal of Endocrine and Metabolic Diseases, 2012; 2: 82-8.

Syarifuddin NI. Perbandingan daya hambat anitbakteri ekstrak etanol kulit batang kasturi (Mangifera casturi Kosterm) dan ekstrak daun sirih terhadap pertumbuhan Streptococcus mutans strain 2302-UNR Secara In Vitro. Jurnal Pharmascience 2014; 1 (2) : 46-53

Rosyidah K, Nurmuhaimina SA, Komari N, Astuti MD. Aktivitas antibakteri fraksi saponin dari kulit batang tumbuhan kasturi. Alchemy 2010; 1(2): 53-103.

Sutomo, Agustina N, Arnida, Fadilaturrahmah. Studi Farmakognostik dan Uji Parameter Nonspesifik Ekstrak Metanol Kulit Batang Kasturi (Mangifera casturi Kosterm.). Jurnal Pharmascience 2017; 04 (01): 94-101.

Khan I, Kumar N, Pant I, Naria S, Kordaiah P. Activation of TGFbeta pathway by Areca Nut Constituents: A Possible cause of Oral Submucous Fibrosis. PLoS ONE 2012; 7(12): e51806.

Widiyati E. Penentuan adanya senyawa triterpenoid dan uji aktivitas biologis pada beberapa spesies tanaman obat tradisional masyarakat pedesaan Bengkulu. Jurnal Gradien 2006; 2(1): 116-22.

Olczyk PL, Mencner L, Vassev KK. The role of the extracellular matrix component in cutaneous wound healing. BioMed Research International 2014; 1: 1-8.

Tortora GJ. Principles of anatomy and physiology. 14th ed. New Jersey, USA : Wiley; 2013. p. 25-86.

Prud’homme, GJ. Pathobiology of transforming growth factor β in cancer, fibrosis and immunologic disease, and therapeutic considerations. Lab Invest 2007; 87(11): 1077-91.

Zou D, Zhang Z, Ye D, Tang A, Deng L, Han W, Zhao J, Wang S, Zhang W, Zhu C, Zhou J, He J, Wang Y, Xu F, Huang Y, Jiang X. Repair of critical-sized rat calvarial defect using genetically engineered bone marrow-derived mesenchymal stem cells overexpressing hypoxia-inducible factor-1α. Stem Cells 2011; 29(9): 1380-90.

Chen, G., C. Deng, & Y. Li. TGF-β and BMP Signaling in Osteoblast Differentiation and Bone Formation. International Journal of Biological Sciences. 2012. 8(2): 272-88.


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