Comparison of Antifungal Susceptibility Basil Leaves Extract (Ocimum sanctum Linn.), Eugenol, and Nystatin against Isolates of Candida spp. as Important Agent causing Oral Candidiasis in HIV/AIDS Patient
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Background: Oral candidiasis is an infection caused by Candida sp. in areas of the oral mucosa that are often found in HIV/AIDS patients. Increased antifungal resistance, it was important to find new antifungal candidates, especially from natural ingredients, basil leaf extract (Ocimum sanctum Linn.) which had major compound of eugenol that had an antifungal effect in inhibiting of Candida sp.
Purpose: To evaluate the comparison of the antifungal susceptibility of nystatin, basil leaf extract (Ocimum sanctum Linn.) and eugenol against isolates of Candida sp.
Methods: This study examined the comparison of the antifungal susceptibility of nystatin 100 IU, basil leaf extract (Ocimum sanctum Linn.) with doses equivalent to 800 µg/mL and 400 µg/mL eugenol, and eugenol 800 µg/mL and 400 µg/mL against 40 stored isolates of Candida sp. from the oral cavity of HIV/AIDS patient which was reactivated.
Result: The mean inhibition zone of nystatin for all isolates was 22.98 mm, while the mean inhibition zone of eugenol with doses of 800 µg/mL and 400 µg/mL were 17.07 mm and 15.89 mm, and the mean inhibition zone of basil leaf extract (Ocimum sanctum Linn.) with doses equivalent to 800 µg/mL and 400 µg/mL eugenol are 14.87 mm and 14.01 mm. The inhibition zone of basil leaf extract (Ocimum sanctum Linn.) and eugenol was significantly lower than nystatin (p = 0.001; p < 0.05).
Conclusion: The zone of inhibition of nystatin was significantly higher compared to basil leaf extract (Ocimum sanctum Linn.) and eugenol against Candida albicans and Candida non-albicans isolates.
Quindós G, Gil-Alonso S, Marcos-Arias C, Sevillano E, Mateo E, Jauregizar N, et al. Therapeutic tools for oral candidiasis: Current and new antifungal drugs. Med Oral Patol Oral Cir Bucal 2019; 24(2): 172–80.
Rosati D, Bruno M, Jaeger M, Ten OJ, Netea MG. Recurrent vulvovaginal candidiasis: an immunological perspective. Microorganisms 2020; 8(2): 144–59.
Murtiastutik D, Maharani CS, Rahmadewi R, Listiawan MY. Nystatin profile on Candida species in HIV/AIDS patients with oral candidiasis: a phenomenology study. Journal of Pure and Applied Microbiology 2019; 13(4): 2013–19.
Dar MS, Sreedar G, Shukla A, Gupta P, Rehan AD, George J. An in vitro study of antifungal drug susceptibility of Candida species isolated from human immunodeficiency virus seropositive and human immunodeficiency virus seronegative individuals in Lucknow population Uttar Pradesh. Journal of oral and maxillofacial pathology : JOMFP 2015; 19(2): 205–11.
Cadena MK, Marcos-Arias C, Mateo E, Aguirre JM, Quindós G, Eraso E. Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis. Arch Oral Biol 2018; 95(1): 100–7.
Rauseo AM, Coler-Reilly A, Larson L, Spec A. Hope on the horizon: novel fungal treatments in development. Open Forum Infectious Diseases 2020; 7(2): 5–23.
De Ornay AK, Prehananto H, Dewi ASS. Growth inhibition of Candida albicans and power kill Candida albicans extract basil leave. Jurnal Wiyata 2017; 4(1): 78–83.
Núñez IC, Arranz JC, Rivas CB, Mendonça PM, Perez K, Sánchez CD, et al. Chemical composition and toxicity of O. sanctum L. var. cubensis essential oil up-growing in the Eastern of Cuba. Phytopathology 2017; 9(7): 1021–28.
Sharifzadeh A, Shokri H. In vitro synergy of eugenol on the antifungal effects of voriconazole against Candida tropicalis and Candida krusei strains isolated from the genital tract of mares. Equine Veterinary Journal 2020; 3(1): 94–101.
Patil S, Majumdar B, Sarode SC, Sarode GS, Awan KH. Oropharyngeal candidosis in HIV-Infected patients”an update. Frontiers in Microbiology 2018; 1(1): 1–9.
Lydiawati E, Listiawan MY, Murtiastutik D, Rahmadewi R, Prakoeswa CRS, Avanti C, et al. In vitro antifungal susceptibility testing of tea tree oil (TTO) 5% compared with nystatin against Candida sp. as important agent of oral candidiasis in HIV/AIDS patients. BIKKK 2020; 32(3) 189–94.
Silva ICG, Santos HBP, Cavalcanti YW, Nonaka CFW, Sousa SA, Castro RD. Antifungal activity of eugenol and its association with nystatin on Candida albicans. Pesquisa Brasileira em Odontopediatria e Clínica Integrada 2017; 17(1): 1–8.
Khan M, Ahmed J, Gul A, Ikram A, Lalani FK. Antifungal susceptibility testing of vulvovaginal Candida species among women attending antenatal clinic in tertiary care hospitals of Peshawar. Infection and Drug Resistance 2018; 11: 447–56.
Nenoff P, Kruger C, Neumeister C, Schwantes U. In vitro susceptibility testing of yeasts to nystatin – low minimum inhibitory concentrations suggest no indication of in vitro resistance of Candida albicans, Candida species or non-Candida yeast species to nystatin. Clin Med Invest 2016; 1(3): 71–6.
Khan A, Ahmad A, Khan LA, Manzoor N. Ocimum sanctum (L.) essential oil and its lead molecules induce apoptosis in Candida albicans. Res Microbiol 2014; 165(6): 411–9.
Brajawikalpa RS, Ramzy AN. Uji efektivitas antijamur minyak atsiri daun cengkeh (Syzygium aromaticum L.) terhadap pertumbuhan Malassezia furfur. Jurnal Kedokteran dan Kesehatan 2018; 4(1): 49–51.
Nejad MS, Özgüneş H, Başaran N. Pharmacological and toxicological properties of eugenol. Turk J Pharm Sci 2017; 14(2): 201–6.
Andayani, A., Susilowati, A., dan Pangastuti, A. 2014. AntiCandida minyak atsiri lengkuas putih (Alpinia galanga) terhadap Candida albicans penyebab candidiasis secara invitro. El Vivo. 2(2): 1–9.
De Paula SB, Bartelli TF, Di Raimo V, Santos JP, Morey AT, Bosini MA, et al. Effect of eugenol on cell surface hydrophobicity, adhesion, and biofilm of Candida tropicalis and Candida dubliniensis isolated from oral cavity of HIV-Infected patients. Evidence-Based Complementary and Alternative Medicine 2014; 1(1): 1–8.
Hsu CC, Lai WL, Chuang KC, Lee MH, Tsai YC. The inhibitory activity of linalool against the filamentous growth and biofilm formation in Candida albicans. Medical Mycology 2013; 51(5): 473– 82.
Raut JS, Shinde RB, Chauhan NM, Mohan KS. Terpenoids of plant origin inhibit morphogenesis, adhesion, and biofilm formation by Candida albicans. Biofouling 2013; 29(1): 87–96.
Jafri H, Banerjee G, Khan MSA. Synergistic interaction of eugenol and antimicrobial drugs in eradication of single and mixed biofilms of Candida albicans and Streptococcus mutans. AMB Expr 2020; 1(10): 185.
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