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  1. High-calorie diet influence with interval restrictions combination on the reproductive cycle and weight of the uterus in mice were analyzed.
  2. Calories balance impact for female reproductive health.

Abstract :

Balanced calories affected for body health and daily activities. Lose of energy can disturb the health and daily activities, while over calorie for diets also causes disorders such as metabolic. When there are excess calories in the body will be involved. It can be an obesity risk, diabetes mellitus, fertility disorder, cancer, and cardiovascular disease. Furthermore, in women there can be an increase in menstrual disorders and fertility disorder risk (Silvestris et al., 2018). This study aims to analyze highcalorie diet influence with interval restrictions combination on the reproductive cycle and weight of the uterus in mice. Experimental laboratory is a mouse (mus musculus) female’s balb/c as object population. Sampling techniques using probability sampling with simple random sampling type. The study results on fisher's exact test gave a result of p > 0.05 so there was no significant difference between control group, high calorie diet group, and high calorie diet with interval restrictions group on the results of vaginal swabs /post-treatment reproductive cycle. The study outcome on robust tests between control, high calorie diet, and high calorie diet with interval restrictions groups. Obtained results (p < 0.05) that mean there are some significant discrepancies in the weight of uterine organ between groups. Calories balance in the body can impact female reproductive health.


High-calorie diet restriction health risk reproductive health dextrose

Article Details

How to Cite
Kumalasari, A. D., Herawati, L., Argarini, R., Lukitasari, L., Othman, Z., & Ningrum, A. G. (2021). High Calorie Diet with a Combination of Intermittent Restriction Affects the Reproductive Cycle and The Uterine Weight of Mice (Mus Musculus). Folia Medica Indonesiana, 57(4), 317–323.


  1. Cheng Y, Lv Q, Xie B, et al (2018). Endometrial mRNA profile in E2 and HFD mice Estrogen and high-fat diet induced alterations in C57BL/6 mice endometrial transcriptome profile. China : endocrine connection. Endocrine Connections 7, 36-46.
  2. Herawati L, Sari GM, Irawan R (2020). High glycemic index diet decreases insulin secretion without altering akt and pdx1 expression on pancreatic beta cells in mice. Chiang Mai University Journal of Natural Sciences 19, 366-378.
  3. Herawati L, Wigati KW, Wibowo W, et al (2016). Survival rate of animal subject to interval and continuous addition of high calorie diet. International Symphosium on Global Physiology and 25th Indonesian Physiological Society ((IPS/IAIFI) Annual Meeting, 42-48.
  4. Hua L, Feng B, Huang L, et al (2020). Time-restricted feeding improves the reproductive function of female mice via liver fibroblast growth factor 21. Clinical and Translational Medicine 10, 1-14.
  5. Hussain MA, Abogresha NM, Hassan R, et al (2016). Effect of feeding a high-fat diet independently of caloric intake on reproductive function in diet-induced obese female rats. Arch Med Sci 12, 906-914.
  6. Lenert ME, Chaparro MM, Burton MD (2021). Homeostatic regulation of estrus cycle of young female mice on western diet. Journal of the Endocrine Society 5, 1-12.
  7. Markowiak P, Slizewska K (2017). Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients 9, 1-30.
  8. McGrice M, Porter J (2017). The effect of low carbohydrate diets on fertility hormones and outcomes in overweight and obese women: A systematic review. Nutrients 9, 1-11.
  9. Narulita E, Prihatin J, Anam K, et al (2017). Perubahan kadar estradiol dan histologi uterus mencit (Mus musculus) betina dengan induksi progesteron sintetik. Biosfera 34, 117-122.
  10. Pósa A, Szabó R, Kupai K, et al (2015). Exercise training and calorie restriction influence the metabolic parameters in ovariectomized female rats. Oxidative Medicine and Cellular Longevity 2015, 1-8.
  11. Ramadhaniah R, Julia M, Huriyati E (2014). Durasi tidur, asupan energi, dan aktivitas fisik dengan kejadian obesitas pada tenaga kesehatan puskesmas. Jurnal Gizi Klinik Indonesia 11, 85-96.
  12. Silvestris E, de Pergola G, Rosania R, et al (2018). Obesity as disruptor of the female fertility. Reproductive Biology and Endocrinology 16, 1-13.
  13. Sitoayu L, Pertiwi DA, Mulyani EY (2017). Kecukupan zat gizi makro, status gizi, stres, dan siklus menstruasi pada remaja. Jurna; Gizi Klinik Indonesia 13, 121-128.
  14. Słuczanowska-Głąbowska S, Laszczyńska M, Piotrowska K, et al (2015). Caloric restriction increases ratio of estrogen to androgen receptors expression in murine ovaries--potential therapeutic implications. J Ovarian Res 8, 1-8.
  15. Solon-Biet SM, Walters KA, Simanainen UK, et al (2015). Macronutrient balance, reproductive function, and lifespan in aging mice. Proceedings of the National Academy of Sciences of the United States of America 112, 3481-3486.
  16. Thiyagarajan DK, Basit H, Jeanmonod R (2019). Physiology, Menstrual cycle. StatPearls, United States.
  17. Weihua Z, Saji S, Mäkinen S, et al (2000). Estrogen receptor (ER) beta, a modulator of ERalpha in the uterus. Proc Natl Acad Sci USA 97, 5936-5941.
  18. Xie L-L, Zhao Y-L, Yang J, et al (2020). Electroacupuncture prevents osteoarthritis of high-fat diet-induced obese rats. Biomed Res Int 2020, 1-16.
  19. Young SL (2013). Oestrogen and progesterone action on endometrium: A translational approach to understanding endometrial receptivity. Reprod Biomed Online 27, 1-17.

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