Nigella sativa EXTRACT IMPROVES SEMINIFEROUS TUBULE EPITHELIAL THICKNESS IN LEAD ACETATE-EXPOSED BALB/C MICE

Alis Nur Diana, Reny I’tishom, Sri Agus Sudjarwo

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Abstract


Lead that enters the body may lead to increased production of ROS (Reactive Oxygen Species) that may affect reproductive system. Black cumin (Nigella sativa) extract contains high antioxidant, tymoquinone, that may be used to suppress oxidative stress induced by lead in animal experiments. This study aimed to prove that black cumin (Nigella sativa) extract improves the thickness of seminiferous tubular epithelium in Balb/c mice exposed to lead (Pb) acetate. This study used post-test only control group design. The subjects in this study were 30 Balb/c male mice (Mus musculus), divided into 5 groups. K-group: control group without lead acetate and black cumin extract, K+ group: group with 50 mg/kgBW of lead acetate for 28 days, P1 group: treatment group with 50 mg/kgBW of lead acetate for 28 days + 0.3 mg/gBW of black cumin extract for 21 days, P2 group: treatment group with 50 mg/kgBW of lead acetate for 28 days + 0.6 mg/gBW of black cumin extract for 21 days, and P3 group: treatment group with 50 mg/gBW of lead acetate for 28 days + 1.2 mg/gBW black cumin extract for 21 days. The results showed that the mean ± standard deviation of the highest thickness of seminiferous tubular epithelium was 45.80 ± 2.73 in the group that was exposed to acetate + 0.6 mg/gBW of black cumin (P2), and the lowest was 32.75 ± 4.07 in the group that was exposed to lead acetate (K+). The results were then analyzed by Anova test. The results showed that there was significant differences in the thickness of seminiferous tubular epithelium between P1, P2, P3 and K+. In conclusion, black cumin extract administration was proved to improve the thickness of seminiferous tubular epithelium in lead (Pb) acetate-exposed Balb/c mice (Mus musculus).

Keywords


Lead acetate; black cumin; thickness of seminiferous tubular epithelium

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References


Abdel-Raheem IT (2010). Greentea Ameliorates Renal Oxidative Damage Induced By Gentamicin In Rats. Pak Journal Pharmaceutical Science, 21-28

Apriliani M, Nurcahyani N, Busman H (2013). Efek pemaparan kebisingan terhadap jumlah sel-sel spermatogenik dan diameter tubulus seminiferus mencit (Mus musculus L.), Seminar Nasional Sains & Teknologi V, Lampung, Lembaga Penelitian Univer-sitas Lampung

Elgawish RAR, Abdelrazek HMA (2014). Effect of lead acetate on testiscular function and caspase-3 express-ion with respect to the protective effect of cinnamon in albino rats. Toxicology Reports 1, 795-801

Flora G, Gupta D, Tiwari A (2012). Toxicity Of Lead: A review with recent updates. Interdiscipline toxicology 5, 47-58

Hayati A (2011). Spermatologi. Surabaya: Pusat Pener-bitan Dan Percetakan Unair

Hsu PC, Guo YL (2002). Antioxidant Nutrients and Lead Toxicity. Toxycology 180: 33-34.

Iswara RAFW (2010). Pengaruh pemberian ekstrak daun cyclea barbata L. miers terhadap motilitas spermatozoa mencit balb/c jantan yang dipapar asam rokok. Artikel Karya Tulis Ilmiah. Denpasar, Univer-sitas Udayana

Kawatu PAT (2008). Kadar Timbal Darah, Hipertensi, Dan Perasaan Kelelahan Kerja Pada Petugas Stasiun Pagi Siang Bahan Bakar Umum Di Kota Manado. Tesis. Yogyakarta, Universitas Gadjah Mada

Lamando D, Soegianto A, Abadi A, Keman S (2014). Antioxidant effects of sarang semut (myrmecodia pendans) on the apoptosis of spermtogenic cells of rats exposed to plumbum. Research Journal of Pharmaceu-tical, Biological and Chemical Sciences 5, 282-294

Laila NN, Shofwati I (2013). Kadar timbal darah dan keluhan kesehatan pada operator wanita SPBU. Jurnal Kesehatan Reproduksi 4, 41-49

Madiha M, Makhlouf M, Eldien HMS, et al (2008). The effect of lead acetate on testiscular structure and protective effect of vitamin E in Adult Albino Rat. Journal of Histology 31, 406-418

Maneesh M, Jayaleksmi H (2006). Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction. International Journal Clinical Biochemistry 21, 80-89

Mayo (2005). Diseases and Conditions; Infertility. Mayo Foundation f or Medical Education and Research (MFMER), December 07; 1-3. Available from: http://www.cnn.com/HEALTH/library/DS/-00310.html.

Riyadina W (1997). Pengaruh Pencemaran Pb terhadap Kesehatan. Pusat penelitian Penyakit Tidak Menular. Badan Penelitian dan Pengembangan Kesehatan Depkes RI. Jakarta.

Sokol RZ, Wang S, Wan YJ, et al (2002). Long-term, low-dose lead exposure alters the gonadotropin-releasing hormone system in the male rat. Environmen Health Perspective 110, 871-874


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