The Effect of Mimosa Pudica Root Extract on Cerebrum Histopathological of Rattus Norvegicus Induced with Naja Sputatrix Venom
Downloads
The aim of this study was to know the effect of Mimosa pudica root extract on histopathological appearance of Rattus norvegicus brain induced by Naja sputatrix venom. Thirty rats were divided into 5 groups. There were 2 control groups and 3 treatment groups, which was given 250, 500, and 1000 mg/kg BW of Mimosa pudica root extract orally. The first 7 days each group was adapted to the environment. On the 8th day, the treatment was started by injecting Naja sputatrix LD50 (0,13 ïL/gram BW) IM in gluteus muscle, continued with giving Mimosa pudica root extract orally for the treatment groups 5 minutes after venom injection. 6 hours after the last treatment, rats were killed by cervical dislocation, injected with formalin 10% in the heart, then necropsied. Histopathological evaluation was done to score brain damage based on meningitis, perivascular cuffing, and necrotic cells using HE stain with 1000x magnification. The result showed 1000 mg/kg BW dosage of Mimosa pudica root extract can reduce brain damage based on meningitis, perivascular cuffing, and necrotic cells in Rat (Rattus norvegicus) caused by Naja sputatrix venom and gave significant difference (p < 0.05) among the treatment groups.
Aird, S. D. 2002. Ophidian envenomation strategies and the role of purines. Toxicon 40:335-393. 2005. Taxonomic distribution and quantitative analysis of free purine and pyrimidine nucleosides in snake venoms. Comp. Biochem. Physiol. B 140:109-126.
Alam, M.I., and Gomes. A. 2003. Snake venom neutralization by Indian medicinal plants (Vitex negundo and Emblica officinalis) root extracts. J. Ethnopharmacology. 86: 75–80.
Alirol, E.; Sharma, S.K.; Bawaskar, H.S.; Kuch, U. & Chappuis, F. 2010. Snake bite in South Asia: A review. PLoS Neglected Tropical Diseases, Vol.4, No.1, pp. e603.
Ambikabothy J, Ibrahim H, Ambu S, Chakravarthi S, Awang K, Vejayan J. 2011. Efficacy evaluations of Mimosa pudica tannin isolate (MPT) for its anti- ophidian properties. J Ethnopharmacol;137:257-62.
Azmi L., Singh M.K. and Akhtar A.K. 2011. Pharmalogical and biological overview on Mimosa pudica Linn. Int. J. of Pharm. & Life Sci. (IJPLS), Vol. 2, Issue 11: Nov.: 1226-1234.
Bancroft JD, 2008. Gamble M. Theory and Practice of Histological Techniques. Nottingham, United Kingdom: Churchill Livingston. pp. 352– 360.
Bieber, A. L. 1979. Metal and nonprotein constituents in snake venoms. In Snake venoms, handbook of exprimental pharmacology, 52, ed. C. Y. Lee, 295–
Berlin: Springer-Verlag.
Bjarnason J.B. and Fox, J.E. 1989. Hemorrhagic toxins from snake venoms. J. Toxicol. Toxin Rev. 7: 121-209.
Bum EN, Dawack DL, Schmutz M, Rakotonirina A, Rakotonirina SV, Portet C. 2004. Anticonvulsant activity of Mimosa pudica decoction. Fitoterapia;75: 309-14.
Cheng, Q.; Feng, J.; Chen, J.; Zhu, X.; Li, F. 2008. Brain transport of neurotoxin-I with PLA nanoparticles through intranasal administration in rats: a microdialysis study. Biopharm. Drug Dispos. 29(8), 431-439.
Chippaux J.P., Williams, White J. 1991. Snake venom variability: methods of study, results and interpretation. Toxicon; 29: 1279.
Chippaux, J.P. 1998. Snake-bites: appraisal of the global situation. Bulletin of the World Health Organization, Vol.76, No.5, pp. 515-524.
Dnyaneshwar D. Kokane, Rahul Y. More, Mandar B. Kale, Minakshi N. Nehete, Prachi C. Mehendale, Chhaya H. Gadgoli. 2009. Evaluation of wound healing activity of root of Mimosa pudica. Department of Pharmacognosy, SVB's College of Pharmacy, Sonarpada, Kalyan-Shil road, Dombivli (E), Dist-Thane, Maharashtra, Pin code 421204, India. (page 312)
Edn.,W.B.Saunders Co., Philadelphia, London.Pp.72. Osweiler, G.D. 1996: Toxicology, The National Veterinary Medicine Series for Independent Study, Williams and wilkins, Philadelpia, Pp.440.
Girish K.S., Mohanakumari H.P., Nagaraju S., Vishwanath B.S., Kemparaju K. 2004. Hyaluronidase and protease activities from Indian snake venoms: neutralization by Mimosa pudica root extract. Fitoterapia. ;75(3–4):378– 380.
Gomes, R.T.; Camargos, R.P.; Viotti, A.P.; Tavares, A.P.; Revelo, M.P.; Freitas,
T.V. 2002. Comparison of the biodistribution of free or liposome-entrapped Crotalus durissus terrificus (South American rattlesnake) venom in mice. Comp. Biochem. Physiol. C Toxicol. Pharmacol., 131(3), 295-301.
Gutiérrez, J. M., and Rucavado A. 2000. Snake venom metalloproteinases: their role in the pathogenesis of local tissue damage. Biochimie 82:841-850.
Hayes A.W. and Kruger C.L. 2004. Hayes' Principles and Methods of Toxicology, 6th ed. (Boca Raton, FL: CRC Press, 2014).
Iwanaga, S., and Suzuki T. 1979. Enzymes in snake venom. In Snake venoms, handbook of experimental pharmacology, 52, ed. C. Y. Lee, 61–158. Berlin: Springer-Verlag.
Joseph B., George J. and Mohan J. 2013. Pharmacology and Traditional Uses of
Mimosa pudica. IJPSDR April-June, Vol. 5, Issue 2: 41-44.
Kamiguti, A.S., Theakston, R.D., Sherman, N. and Fox, J.W. 2000. Mass spectrophotometric evidence for P-III/P-IV metalloproteinases in the venom of the Boomslang (Dispholidus typus) Toxicon 38: 1613–1620.
Kasturiratne, A.; Wickremasinghe, A.R.; de Silva, N.; Gunawardena, N.K.; Pathmeswaran, A.; Premaratna, R.; Savioli, L.; Lalloo, D.G. & de Silva,
H.J. 2008. The global burden of snakebite: a literature analysis and modeling based on regional estimates of envenoming and deaths. PLoS Medicine, Vol.5, No.11, pp. e218
Kennedy P. G. E., Rodgers J., Jennings F. W., Murray M., Leeman S. E. and Burke
J. M. 1997. A substance P antagonist, RP-67,580, ameliorates a mouse meningoencephalitic response to Trypanosoma brucei brucei. Proc. Natl. Acad. Sci. USA. Vol. 94; April.: pp. 4167–4170.
Kini, R. M. 1997. Venom Phospholipase A2 Enzymes: Structure, Function and Mechanism. Wiley, New York, New York.
Kokane DD, More RY, Kale MB, Nehete MN, Mehendale PC, Gadgoli CH. 2009. Evaluation of wound healing activity of root of Mimosa pudica. J Ethnopharmacol;124:311-5.
Kumar B. K, Nanda S.S., Venkateshwarlu P., Kumar Y. K., Jadhav R.T. 2010. International Journal on Pharmaceutical and Biomedical Research (IJPBR) Vol. 1(3), 2010, 76-89.
Lyonel GI., Esther DI. 1999. Apoptosis. Stem cells, 17, 306-13.
Mackessy, S. P. 1998. Phosphodiesterases, DNases and RNases. In Enzymes from snake venoms, ed. G. S. Bailey, 361–404. Ft. Collins, CO: Alaken Press.
Mahanta M., Mukherjee A. K. 2001. Neutralisation of lethality, myotoxicity and toxic enzymes of Naja kaouthia venom by Mimosa pudica root extracts. Journal of Ethnopharmacology 75: 55–60.
Maya Devi C, Vasantha Bai Mary, Vijayan Lal Arthur, Umashankar PR and Krishnan Lissy K. 2002. An improved method for isolation of antiviper venom antibodies from chicken egg yolk. J. Biochem. Biophys. Methods. 51, 129– 138.
Mescher, A.L. 2010. Junqueira's Basic Histology Text and Atlas. Thirteenth Edtition. Mc Graw-Hill Education. New York. 1-559.
Mount, M. E. 1989. Toxicology, S.J. Ettinger (ed.), In text book of Veterinary Internal Medicine, Vol. 1, 3rd Edn.,W.B.Saunders Co., Philadelphia, London.Pp.72.
Mukherjee PK, Kumar V, Mal M, Houghton PJ. 2007. Acetylcholinesterase inhibitors from plants. Phytomedicine;14:289-300.
Nam KN, ParkY-M, Jung H-J, Lee JY, Min BD, Park S-U. 2010. Anti- inflammatory effects of crocin and crocetin in rat brain microglial cells. Eur J Pharmacol pp;648:110–6.
Omran M. A. A., Fabb S.A., Dickson G. 2004. Biochemical and morphological analysis of cell death induced by Egyptian cobra (Naja haje) venom on cultured cells. J. Venom. Anim. Toxins incl. Trop. Dis, 10, 3, p.220.
Peichoto, M.E., Teibler, P., Ruiz, R., Leiva, L. and Acosta, O. 2006. Systemic pathological alterations caused by Philodryas patagoniensis collubrid snake venom in rats. Toxicon 48: 520– 528.
Pierre Golstein and Guido Kroemer. 2006. Cell death by necrosis: towards a molecular definition
Prashanth, D., Vinutha, B., Salma, K., Sreeja, S.L., Pratiti, D., Padmaja, R., Radhika, S., Amit, A., Vankateshwarlu, K., Deepak, M., 2007. Screening of selected Indian medicinal plants for acetyl cholinesterase inhibitory activity. Journal of Ethnopharmacology 109, 359–363.
Rael, E. D. 1998. Venom phosphatases and 5"² nucleotidase. In Enzymes from snake venoms, ed. G. S. Bailey, 405–22. Ft. Collins, CO: Alaken Press.
Rania H. Abdou & Abdelaziz E. Ibrahim. 2015. Effects of Egyptian Cobra (Naja haje) venom on Post Mortem Changes and Some Biochemical Parameters in Rats. Department of forensic medicine and toxicology, Faculty of Vet. Medicine, Suez Canal University, Ismailia, Egypt.
Schuchat A, Robinson K, Wenger JD. 1997. Bacterial meningitis in the United States in 1995. Active Surveillance Team. N Engl J Med: 337:970 – 6.
Shukla, P.C. 2009. Snake bite in animals and its treatment.Pashudhan, 35(2):2,4. Uetz, P., Ho sek, J., 2015. The Reptile Database. http://www.reptile-database.org.
Accessed January 26, 2015.
Vejayan J., Ibrahim H. and Othman I. 2007. The Potential Of Mimosa Pudica (Mimosaceae) Against Snake Envenomation. Journal of Tropical Forest Science 19(4): 189–197.
Wallach, V., Wüster, W., Broadley, D.G., 2009. In praise of subgenera: taxonomic status of cobras of the genus Naja Laurenti (Serpentes: Elapidae). Zootaxa 2236, 26e36.
White, J., 2000. Bites and stings from venomous animals: a global overview. Drug Monit. 22 (1), 65–68.
World Health Organization, 2007. Rabies and Envenomings. A Neglected Public Health Issue. World Health Organization, Geneva.
Yap, M.K.K., Tan, N.H., Fung, S.Y., 2011. Biochemical and toxinological characterization of Naja sumatrana (Equatorial spitting cobra) venom. J. Venom. Anim. Toxins Incl. Trop. Med. 17, 451–459.
Zhan, C.; Yan, Z.; Xie, C.; Lu, W. 2010. Loop 2 of Ophiophagus Hannah toxin b binds with neuronal nicotinic acetylcholine receptors and enhances intracranial drug delivery. Mol. Pharm., 7(6), 1940-1947.
Zhang, H.L.; Han, R.; Chen, Z.X.; Chen, B.W.; Gu, Z.L.; Reid, P.F.; Raymond, L.N.; Qin, Z.H. 2006. Opiate and acetylcholineindependent analgesic actions of crotoxin isolated from Crotalus durissus terrificus venom. Toxicon, 48(2), 175-182.
Copyright (c) 2022 Roselia Yuliani Permatasari, Eka Pramyrtha Hestianah, Djoko Legowo, Kadek Rachmawati, Sri Agus Sudjarwo, Zainal Arifin
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Journal of Basic Medical Veterinary (JBMV) by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
Perhimpunan Dokter Hewan Indonesia
