Students’ Habits and Identification of Bacteria on Inanimate Surface of Educational Setting
Introduction: Diponegoro Clinic reported 29.1% of students seek infectious diseases treatment. Student habits in the classroom were thought to play roles in the presence of bacteria. Surfaces of inanimate objects in the classroom were potential source for bacteria. The research objectives were analyzing student’s habits and identifying bacteria on the surface of inanimate objects in the classroom. Methods: Four types of samples, including the surfaces of tables, chairs, flips of air conditioners, and floors were taken from 13 faculties at Universitas Diponegoro. Plate count agar media were used to isolate bacterial colonies, and PCR analysis was performed for DNA extraction and amplification. DNA Sanger sequencing techniques were used for genetic bacterial identification. The online questionnaire was used to assess student habits in the classroom. Two hundred students responded. Results and Discussion: Acinetobacter baumannii and Staphylococcus aureus were found in classrooms. These bacteria were associated with respiratory tract infections. This study revealed that 86.5% were between the ages of 17-21, 60.81% were from outside Semarang City, and 88.33% lived in Semarang City. About 60.81% of respondents studied in health sciences. Furthermore, it was reported that 66.67% of respondents were sick in the last few weeks, attended class despite being sick (72.52%), and coughed and sneezed in class (40.99%). Conclusion: Bacteria associated with respiratory tract infection were found. Students' habits in the classroom were potentially caused by the presence of these bacteria. The use of antibacterial agents could reduce the presence of bacteria on inanimate object surfaces.
Buppan P, Sommatas A, Nakprasom P, Noicharoen N, Sangsuvan D. Identification of Bacteria and Fungi Contamination of Some Classrooms at Srinakharinwirot University, Ongkharak Campus. Asian J Appl Sci. 2019;7(5):557–561. https://doi.org/10.24203/ajas.v7i5.5983
Endo A, Uchida M, Hayashi N, Liu Y, Atkins KE, Kucharski AJ, et al. Within and Between Classroom Transmission Patterns of Seasonal Influenza Among Primary School Students in Matsumoto city, Japan. Proc Natl Acad Sci. 2021;118(46):1–8. https://pnas.org/doi/full/10.1073/pnas.2112605118
Almatawah QA, Al-Rashidi MS, Yassin MF, Varghese JS. Microbiological Contamination of Indoor and Outdoor Environments in A Desert Climate. Environ Monit Assess. 2022;194(5):1-16. https://doi.org/10.1007/s10661-022-10032-9
Bhatta DR, Hamal D, Shrestha R, Hosuru Subramanya S, Baral N, Singh RK, et al. Bacterial Contamination of Frequently Touched Objects in A Tertiary Care Hospital of Pokhara, Nepal: how safe are our hands? Antimicrob Resist Infect Control. 2018;7(1):1-6. https://aricjournal.biomedcentral.com/articles/10.1186/s13756-018-0385-2
Gonzalez-Martin C. Airborne Infectious Microorganisms. In: Reference Module in Life Sciences. Elsevier. 2019;4:52–60. https://linkinghub.elsevier.com/retrieve/pii/B978012809633813002X
Querido MM, Aguiar L, Neves P, Pereira CC, Teixeira JP. Self-Disinfecting Surfaces and Infection Control. Colloids Surfaces B Biointerfaces. 2019;178(1):8-21. https://doi.org/10.1016/j.colsurfb.2019.02.009
Ascione F, De Masi RF, Mastellone M, Vanoli GP. The Design of Safe Classrooms of Educational Buildings for Facing Contagions and Transmission of Diseases: A Novel Approach Combining Audits, Calibrated Energy Models, Building Performance (BPS) and Computational Fluid Dynamic (CFD) Simulations. Energy Build. 2021;230(1):1-28. https://doi.org/10.1016/j.enbuild.2020.110533
Nurudeen ASN, Toyin A. Knowledge of Personal Hygiene among Undergraduates. J Heal Educ. 2020;5(2):66–71. https://journal.unnes.ac.id/sju/index.php/jhealthedu/article/view/38383
O. Stanley H, C. Anele B, O. Okerentugba P, M. Immanuel O, J. Ugboma C. Isolation and Molecular Identification of Biodeteriogens Isolated from Painted Classroom Wall Surfaces in University of Port Harcourt, Nigeria. South Asian J Res Microbiol. 2020;5(3):1–6. https://journalsajrm.com/index.php/SAJRM/article/view/88
Rai S, Singh DK, Kumar A. Microbial, Environmental and Anthropogenic Factors Influencing The Indoor Microbiome of The Built Environment. J Basic Microbiol. 2021;61(4):267–292. https://onlinelibrary.wiley.com/doi/10.1002/jobm.202000575
Swamy GSNVKSN. Development of An Indoor Air Purification System to Improve Ventilation and Air Quality. Heliyon. 2021;7(10):1-7. https://doi.org/10.1016/j.heliyon.2021.e08153
Ting Wu H, Shuang Li Q, Chen Dai R, Liu S, Wu L, Mao W, et al. Effects of Air-Conditioning Systems In The Public Areas Of Hospitals: A Scoping Review. Epidemiol Infect. 2021;149(1):1-11. https://doi.org/10.1017/S0950268821001990
Dai R, Liu S, Li Q, Wu H, Wu L, Ji C. A Systematic Review and Meta-Analysis of Indoor Bioaerosols In Hospitals: The Influence Of Heating, Ventilation, and Air Conditioning. PLoS One. 2021;16(12):1-24. http://dx.doi.org/10.1371/journal.pone.0259996
Cavicchioli R, Ripple WJ, Timmis KN, Azam F, Bakken LR, Baylis M, et al. Scientists’ Warning To Humanity: Microorganisms And Climate Change. Nat Rev Microbiol. 2019;17(9):569–586. http://dx.doi.org/10.1038/s41579-019-0222-5
Scott EA, Bruning E, Nims RW, Rubino JR, Ijaz MK. A 21st Century View of Infection Control In Everyday Settings: Moving from the Germ Theory of Disease to the Microbial Theory of Health. Am J Infect Control. 2020;48(11):1387–1392. https://doi.org/10.1016/j.ajic.2020.05.012
Abdi AM, Amano A, Abrahim A, Getahun M, Ababor S, Kumie A. Food Hygiene Practices and Associated Factors Among Food Handlers Working In Food Establishments In The Bole Sub City, Addis Ababa, Ethiopia. Risk Manag Healthc Policy. 2020;13(1):1861–1868. https://doi.org/10.2147/RMHP.S266342
Diponegoro Pratama Clinic. Laporan Penyakit Tertinggi Januari-Juli 2023. Semarang: Diponegoro Pratama Clinic; 2023.
Katzenberger RH, Rösel A, Vonberg RP. Bacterial Survival on Inanimate Surfaces: A Field Study. BMC Res Notes. 2021;14(1):1–10. https://doi.org/10.1186/s13104-021-05492-0
Tamura K, Stecher G, Kumar S. MEGA11 : Molecular Evolutionary Genetics Analysis Version 11. 2021;38(7):3022–3027. https://doi.org/10.1093/molbev/msab120
Sun Y, Meng Y, Ou Z, Li Y, Zhang M, Chen Y, et al. Indoor Microbiome, Air Pollutants and Asthma, Rhinitis And Eczema In Preschool Children – A Repeated Cross-Sectional Study. Environ Int. 2022;161(1):1-13. https://linkinghub.elsevier.com/retrieve/pii/S0160412022000630
Pivard M, Moreau K, Vandenesch F. Staphylococcus aureus Arsenal to Conquer the Lower Respiratory Tract. mSphere. 2021;6(3):1-17. https://journals.asm.org/doi/10.1128/mSphere.00059-21
Darge A, Kahsay AG, Hailekiros H, Niguse S, Abdulkader M. Bacterial Contamination and Antimicrobial Susceptibility Patterns of Intensive Care Units Medical Equipment and Inanimate Surfaces at Ayder Comprehensive Specialized Hospital, Mekelle, Northern Ethiopia. BMC Res Notes. 2019;12(1):1-8. https://doi.org/10.1186/s13104-019-4658-5
Dang DYN, Vuong HN, Nguyen TT, Phan TTT. Microbiological Contamination of Indoor Air in University Classrooms (Case study: University of Science - Vietnam National University, Ho Chi Minh City). Vietnam J Sci Technol Eng. 2020;62(4):30–35. https://vietnamscience.vjst.vn/index.php/VJSTE/article/view/369/254
Chegini Z, Noei M, Hemmati J, Arabestani MR, Shariati A. The Destruction of Mucosal Barriers, Epithelial Remodeling, and Impaired Mucociliary Clearance: Possible Pathogenic Mechanisms Of Pseudomonas aeruginosa and Staphylococcus aureus in Chronic Rhinosinusitis. Cell Commun Signal. 2023;21(1):1–14. https://doi.org/10.1186/s12964-023-01347-2
Tahir S, Chowdhury D, Legge M, Hu H, Whiteley G, Glasbey T, et al. Transmission of Staphylococcus aureus from Dry Surface Biofilm (DSB) Via Different Types of Gloves. Infect Control Hosp Epidemiol. 2019;40(1):60–64. https://doi.org/10.1017/ice.2018.285
Vestby LK, Grønseth T, Simm R, Nesse LL. Bacterial Biofilm and Its Role in the Pathogenesis of Disease. Antibiotics. 2020;9(2):1-29. https://doi.org/10.3390/antibiotics9020059
Zeidler S, Müller V. Coping with Low Water Activities and Osmotic Stress in Acinetobacter Baumannii : Significance, Current Status and Perspectives. Environ Microbiol. 2019;21(7):2212-2230. https://doi.org/10.1111/1462-2920.14565
Whiteway C, Breine A, Philippe C, Van der Henst C. Acinetobacter baumannii. Trends Microbiol. 2022;30(2):199–200. https://doi.org/10.1016/j.tim.2021.11.008
Hussain HH, Ibraheem NT, Al-Rubaey NKF, Radhi MM, Hindi NKK, Al-Jubori RHK. A Review of Airborne Contaminated Microorganisms Associated with Human Diseases. Medical Journal of Babylon. 2020;19(2):115–122. http://dx.doi.org/10.4103/MJBL.MJBL_20_22
Appaneal HJ, Lopes V V, LaPlante KL, Caffrey AR. Treatment, Clinical Outcomes, and Predictors of Mortality among a National Cohort of Admitted Patients with Acinetobacter baumannii Infection. Antimicrob Agents Chemother. 2022;66(3):1-10. https://doi.org/10.1128/aac.01975-21
Kiros T, Damtie S, Eyayu T, Tiruneh T, Hailemichael W, Workineh L. Bacterial Pathogens and Their Antimicrobial Resistance Patterns of Inanimate Surfaces and Equipment in Ethiopia: A Systematic Review and Meta-analysis. Biomed Res Int. 2021;2021(1):1–25. https://www.hindawi.com/journals/bmri/2021/5519847/
Chen B, Han J, Dai H, Jia P. Biocide-Tolerance and Antibiotic-Resistance In Community Environments and Risk Of Direct Transfers To Humans: Unintended Consequences of Community-Wide Surface Disinfecting During COVID-19? Environ Pollut. 2021;283(1):1-15. https://doi.org/10.1016/j.envpol.2021.117074
Agi VN, Ollor OA, Azike CA, Onwuasoanya C V. Isolation and Identification of Microorganisms from Makeup Brushes in Rivers State University Nigeria and Its Environs. J Adv Microbiol. 2023;23(3):41–50. https://doi.org/10.9734/jamb/2023/v23i3715
AL-Harmoosh R, Eidan A, Naji H, Ahmed W, Mohammad M. Potential Pathogenic Bacterial Contaminants of Doors Handles and Computers Keyboards in the Faculty Environment. J Pure Appl Microbiol. 2019;13(2):975–982. https://dx.doi.org/10.22207/JPAM.13.2.34
Dai R, Liu S, Li Q, Wu H, Wu L, Ji C. A Systematic Review and Meta-Analysis of Indoor Bioaerosols In Hospitals: The Influence Of Heating, Ventilation, and Air Conditioning. PLoS One. 2021;16(12):1-24. https://dx.plos.org/10.1371/journal.pone.0259996
Cheong CH, Hwang S-H, Park B. Ventilation Methods to Prevent The Spread of Airborne Pathogens from Teachers to Students. Int J Air-Conditioning Refrig. 2023;31(1):1-12. https://doi.org/10.1007/s44189-023-00024-w
Jing JLJ, Pei Yi T, Bose RJC, McCarthy JR, Tharmalingam N, Madheswaran T. Hand Sanitizers: A Review on Formulation Aspects, Adverse Effects, and Regulations. Int J Environ Res Public Health. 2020;17(9):1-17. https://www.mdpi.com/1660-4601/17/9/3326
Stephens B, Azimi P, Thoemmes MS, Heidarinejad M, Allen JG, Gilbert JA. Microbial Exchange Via Fomites and Implications for Human Health. Curr Pollut Reports. 2019;5(4):198–213. http://link.springer.com/10.1007/s40726-019-00123-6
Bravo Z, Orruño M, Navascues T, Ogayar E, Ramos-Vivas J, Kaberdin VR, et al. Analysis of Acinetobacter baumannii Survival In Liquid Media and On Solid Matrices As Well As Effect Of Disinfectants. J Hosp Infect. 2019;103(1):e42–e52. https://linkinghub.elsevier.com/retrieve/pii/S0195670119301598
Fakunle AG, Jafta N, Smit LAM, Naidoo RN. Indoor Bacterial and Fungal Aerosols As Predictors of Lower Respiratory Tract Infections Among Under-Five Children in Ibadan, Nigeria. BMC Pulm Med. 2022;22(1):1-14. https://doi.org/10.1186/s12890-022-02271-w
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