Detection of Viral Haemorrhagic Septicemia in Marine Fisheries Commodity Using Real Time–PCR at Fish Quarantine Inspection Agency Denpasar, Bali
Downloads
Marine fish commodities in Indonesia, both exported and imported, have experienced a significant increase. This increase opens up opportunities for the risk of entry and spread of fish pests and diseases, both on an export scale between countries and between areas within Indonesia. One of the pathogens that is acute that can cause major losses is the virus. The virus that often attacks sea water ornamental fish is Viral haemorrhagic septicemia. The purpose of this study was to detect the presence of VHSV that infects marine ornamental fish commodities using namely: necropsy, fixation, extraction, amplification, and reading of results. This research was conducted at Fish Quarantine Inspection Agency Denpasar, Bali. Based on the test results, VHS was found to attack six samples of marine fish species with codes namely NTC, BC 8.2, BC 9.2, BAR 8.2, 1252, NSR 8.2.
Annisa. (2012). Isolasi RNA dan pengklonaan gen tripsin kation dan pankreas sapi ke Escherichia coli DH5α. Skripsi. Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam. Departemen Biologi. Universitas Indonesia.
Ayu. L. (2014). Pengaruh suhu annealing pada program PCR terhadap keberhasilan amplifikasi DNA udang jari (Metapenaus elegan) laguna Segara Anakan Cilacap Jawa Tengah. Thesis. Malang: Fakultas Sains dan Teknologi. Universitas Islam Negeri Maulana Malik Ibrahim Malang.
Flikhah , A. R. I. (2016). Implementasi peraturan Menteri Kelautan dan Perikanan Republik Indonesia Nomor PER. 19/MEN/2010 tentang pengendalian sistem jaminan mutu dan keamanan hasil perikanan (Studi mengenai pelaksanaan pemberian sertifikat ekspor ikan di Balai Karantina Kelas I kota Surabaya Jawa Timur). Jurnal Penelitian Administrasi Publik, 2(2):463-478.
Fitriatin, E. & Manan, A. (2015). Pemeriksaan Viral Nervous Necrosis (VNN) pada ikan dengan metode Polymerase Chain Reaction (PCR). Jurnal Ilmiah Perikanan dan Kelautan, 7(2):149-152.
Gillman, M. (2002). Current protocols in molecular biology. Texas: John Wiley & Sons Inc.
Goodwin, A. E. & Merry G. E. (2011). Replication and persistence of VHSV IVb in freshwater turtles. Diseases of Aquatic Organisms, 94:173-177.
Hanggono. B. & Junaidi, M. (2015). Deteksi penyakit viral pada udang vannamei (Litopennaus vannamei) dengan metode Polymerase Chain Reaction (PCR). Jurnal Ilmu Perikanan, 6(1):4-6.
Hershberger, P.K., Gregg, J.L., & Grady, C.A. (2010). Susceptibility of three stocks of Pacific herring to Viral Hemorrhagic Septicemia. Journal of Aquatic Animal Health, 22(1):1-7.
Kementerian Kelautan dan Perikanan. (2.018, Januari). Produktivitas perikanan Indonesia pada: Forum Merde ka Barat 9 Kementerian Komu nikasi Informatika. Jakarta.
Badan Karantina Ikan. (2017). Keputusan Kepala Badan Karantina Ikan Nomor 73/KEP-BKIPM/2017 tentang pedoman dan ketentuan terkait karantina ikan, pengendalian mutu, dan keamanan hasil perikanan. Jakarta: Badan Karantina Ikan.
Kim, S-H., Thu, B. J., Skall, H. F., Vendramin, N., & Evensen, í¸. (2014). A single amino acid mutation (I1012F) of the RNA polymerase of marine Viral Hemorrhagic Septicemia Virus changes in vitro virulence to rainbow trout gill epithelial cells. Journal of Virology, 88(13):7189-7195.
Kurita, J., Mori, K., & Olesen, J. N. (2016). Virulence of Viral Haemorrhagic Septicemia virus (VHSV) genotype III in rainbrow trout. Journal of Veterinary Research, 47(4):2-4.
Kurniawati, M. D., Sumaryam, & Hayati. N. (2019). Aplikasi Polymerase Chain Reaction (PCR) konvensional dan Real Time - PCR untuk deteksi virus VNN (Viral Nervous Necrosis) pada ikan kerapu macan (Epinephelus fuscoguttatus). Jurnal Technoo-Fish, 3(1):19-30.
Langga, I. F., Restu, M., & Kuswinanti, T. (2012). Optimalisasi suhu dan lama inkubasi dalam ekstraksi DNA tanaman bitti (Vitex cofassus Reinw) serta analisis keragaman genetik dengan teknik RAPD-PCR. Jurnal Sains & Teknologi, 12(3):265-276.
Mahardika, K. Mastuti, I., Roza, D., Syahidah, D., Astuti, W. W., Ismi, S., & Zafran, Z. (2020). Pemantauan insidensi penyakit pada ikan kerapu dan kakap di hatchery dan keramba jaring apung di Bali Utara. Jurnal Riset Akuakultur, 15(2):89-102.
Montero, J., Garcia, J., Ordas, M. C., Casanova, I., Gonzalez, A., Villena, A., Coll, J., & Tafalla, C. (2011). Specific regulation of the chemokine response to Viral Haemorrhagic Septicemia Virus (VHSV) at the entry site. Journal of Virology, 85(9):4046-4056.
Olson, W, J. (2013). Viral Hemorrhagic Septicemia Virus (VHSV) Great Lakes Strain IVb: Viral detection, mechanisms of infection, and host-virus interactions in the yellow perc (Perca flavescens). Theses and Dissertations. University of Wisconsin Milwaukee. UWM Digital Commons. https://dc.uwm.edu/etd/323
Ramlah, E., Soekendarsi, Z., & Hasyim. (2016). Perbandingan kandung an gizi ikan nila Oreochromi s niloticus asal danau Mawang Kabupaten Gowa dan danau Universitas Hasanuddin Kota Makassar.
Warg, J. V., Clement, T. C., Cornwell, E. R., Cruz, A., Getchell, R. G., Giray, C., Goodwin, A. E., Groocock, G. H., Faisal, M., Kim, R., Merry, G. M., Phelps, N. B. D., Reising, M. M., Standish, I., Zhang, Y., & Toohey-Kurth, K. (2014). Detection and surveillance of viral hemorrhagic septicaemia virus using real-time RT-PCR. I. Initial comparison of four protocols. Diseases of Aquatic Organisms, 111:1-13
Copyright (c) 2024 Journal of Marine and Coastal Science
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.