Isolation and Identification of Protein Spike (Protein-S) in Field Isolates of Infectious Bronchitis Virus

Infectious Bronchitis Virus vaccine field isolate Spike Glycoprotein

Authors

  • Jola Rahmahani
    jola_rahmahani@yahoo.co.id
    Laboratorium Virologi dan Immunologi, Divisi Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Airlangga
  • Dewanggi Kristi Sasmi Pradhita Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan Universitas Airlangga
  • Nanik Sianita Widjaja Laboratorium Virologi dan Immunologi, Divisi Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Airlangga
  • Suwarno Suwarno Laboratorium Virologi dan Immunologi, Divisi Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Airlangga
April 15, 2021

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Infectious Bronchitis (IB) is disease causes great economic impact across nations. Spike protein (protein-S) is one of structural protein of Infectious Bronchitis Virus (IBV) located on enveloped of the virion. Molecular characterization of IBV could be conducted use Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to reveal the protein weight then confirmed through Immuno-Blotting assay. These methods were conducted to know the molecule weight of field isolate compared to seed vaccine. The results of this study indicate the weight of protein-S of field isolate I-147/11 were 168.21 kDa which was same to protein-S of seed vaccine Massachusset.

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