Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences
Downloads
The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.
Alim, M. A., Hossain, M. M. K., Nusrat, J., Rubaya, Salimullah, M., Shu-Hong, Z., & Alam, J. (2019). Genetic effects of leptin receptor (LEPR) polymorphism on litter size in a Black Bengal goat population. Animal Biology, 69(4), 411–420.
Anugratama, L. E., & Hartatik, T. (2020). Short communication: Identification of leptin gene in crossbred beef cattle. Biodiversitas, 21(1), 226–230.
Bakhtiar, R., Abdolmohammadi, A., Hajarian, H., Nikousefat, Z., & Kalantar-Neyestanaki, D. (2017). Identification of g.170G>A and g.332G>A mutations in exon 3 of leptin gene (Bcnl and Cail) and their association with semen quality and testicular dimensions in Sanjabi rams. Animal Reproduction Science, 179, 49–56.
Di Gregorio, P., Di Trana, A., Celi, P., Claps, S., & Rando, A. (2014). Comparison of goat, sheep, cattle and water buffalo leptin (LEP) genes and effects of the Intron 1 microsatellite polymorphism in goats. Animal Production Science, 54(9), 1258–1262.
Dzhulamanov, K. M., Lebedev, S. V., Gerasimov, N., & Kolpakov, V. I. (2022). Effect of leptin C528T and leptin C73T polymorphisms and pregnancy on adipose tissue formation and carcass grade in Aberdeen Angus heifers and first-calf cows. Veterinary World, 15(7).
Hartatik, T., Hariyono, D. N. H., & Adinata, Y. (2019). Short communication: Genetic diversity and phylogenetic analysis of two indonesian local cattle breeds based on cytochrome b gene sequences. Biodiversitas, 20(1), 17–22.
Hartatik, T., Latifah, Yuliana, R., & Kustantinah, A. (2020). Genotyping and Chi-Square Analysis of 967 bp Leptin Gene in Bligon Goat. In IOP Conference Series: Earth and Environmental Science, 478.
Hernawati, T., Mulyati, S., & Suprayogi, T. W. (2019, June). Identification on osteopontin promoter gene polymorphism and post-thawing quality in dairy bull peranakan Friesian Holstein. In IOP Conference Series: Materials Science and Engineering, 546(6), 062010.
Hernawati, T., Oktanella, Y., & Dhaneswari, S. A. (2021). Tyrosine Kinase Gene Polymorphisms Associated with Fresh Semen Quality from Dairy Bull Friesian Holstein. Iraqi Journal of Agricultural Sciences, 52(4), 1050-1057.
Jiyanto, J., Sutopo, S., & Kurnianto, E. (2014). The genetic diversity of kejobong goat based on cytochrome b gene. Journal of the Indonesian Tropical Animal Agriculture, 39(2), 75–82.
Latifah, L., Muarifah, H., Sopian, Y., & Guntur, A. (2020). Analisis Genetik Gen KiSS1 pada Kambing Berdasarkan Sekuen DNA GenBank. Journal of Tropical Animal Research (JTAR), 1(01), 20-28.
Madeira, F., Pearce, M., Tive, A.R.N., Basutkar, P., Lee, J., Edbali, O., Madhusoodanan, N., Kolesnikov, A., and Lopez, R. 2022. Search and sequence analysis tools services from EMBL-EBI in 2022. Nucleic Acids Research. 2022 Apr:gkac240. DOI: 10.1093/nar/gkac240. PMID: 35412617; PMCID: PMC9252731.
Meydilasari, N. I., Hernawati, T., Lestari, T. D., Safitri, E., Sardjito, T., Ismudiono, I., & Hidanah, S. (2020). Hubungan antara skor hasil bacaan heat detector dengan gambaran sitologi vagina sebagai parameter penentuan estrus pada kambing. Ovozoa: Journal of Animal Reproduction, 9(1), 7–11.
Münzberg, H., & Heymsfield, S. B. (2019, May 7). New Insights into the Regulation of Leptin Gene Expression. Cell Metabolism. Cell Press.
Nanda, E. R. V., Harijani, N., & Wibawati, P. A. (2020). Uji Total Bakteri Susu Segar Kambing Jawa Randu di Siliragung, Banyuwangi. Jurnal Medik Veteriner, 3(2), 224–229.
NCBI. (2022a). Capra hircus obese gene for leptin, exons 2-3, AM114397.2, Sequence of the gene coding for the goat leptin. Accessed.
NCBI. (2022b). Capra hircus breed Osmanabadi leptin (Leptin) gene, complete CDS, MH716185.1. Accessed.
Pahlevy, J. R., Ratnani, H., Restiadi, T. I., Fikri, F., Saputro, A. L., & Agustono, B. (2022). The addition of vitamin C in tris–egg yolk extender maintained Sapera goat semen quality in 5° C storage. Ovozoa: Journal of Animal Reproduction, 11(1), 1–8.
Sutikno, S., Priyanto, R., Jakaria, J. (2020). Polymorphism g.-371T>A Promoter of the Myostatin Gene in Indonesian Beef Cattle. Jurnal Ilmu Pertanian Indonesia, 25(2), 239–244.
Utomo, B. (2018). Identification Polymorphism of FSH ẞ-Sub Unit Gene and FSHR Gene in Madura Cattle Results of Artificial Insemination with Limousin Cattle with PCR-RFLP Technique. International Journal of Advanced Scientific Research and management, 3(10), 38-43.
Utomo, B., & Safitri, E. (2018). Polymorphism of growth hormone gene in the artificial insemination result of Madura cattle with Limousin semen as a reference for genetic selection. Iraqi Journal of Veterinary Sciences, 32(1), 113-118.
Utomo, B., Rimayanti, R., Triana, I. N., & Fadholly, A. (2021). Melanocortin-4 receptor and leptin as genes for the selection of superior Madrasin cattle. Veterinary World, 14(12), 3224-3228.
Vazir, N. E. A., Koshkooieh, A. E., Mehrjerdi, A. A., Mohammadabadi, M., Babenko, O. I., Bushtruk, M. V., Klopenko, N. I. (2020). Genetic diversity of leptin gene intron 2 in wild and native and exotic goat breeds in Iran. Journal of Microbiology, Biotechnology and Food Sciences, 9(6), 1110–1113.
Wang, P. Q., Deng, L. M., Zhang, B. Y., Chu, M. X., Tan, Y., Fan, Q., & Liu, C. X. (2011). Identification of polymorphism on leptin receptor gene of goats in southwest China. Small Ruminant Research, 96(2–3), 120–125.
Copyright (c) 2023 Muhammad Fajar Amrullah, Budi Utomo, Suzanita Utama, Tri Wahyu Suprayogi, Tita Damayanti Lestari, Tjuk Imam Restiadi, Ristaqul Husna Belgania
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish in this journal agree to the following terms:
1. The journal allows the author to hold the copyright of the article without restrictions;
2. The journal allows the author(s) to retain publishing rights without restrictions;
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA).
11.jpg)
