Protective Effects of Apis Dorsata Honey Supplementation on Kidney Histopathology in Mice with Monosodium Glutamate Exposure
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This study was conducted to investigate the protective effects of Apis dorsata honey on the histopathological changes of the kidney in mice exposed to monosodium glutamate. This study used 25 male mice as the experimental animal which were divided into five groups with five replication, respectively i.e. (C-) was administered basal diet, (C+) was administered 4 mg/g BW MSG, (T1) was administered A. dorsata honey of 53.82 mg/20g BW followed by MSG 4 mg/g BW, (T2) was administered A. dorsata honey of 107.64 mg/20g BW followed by MSG 4 mg/g BW, and (T3) was administered A. dorsata honey of 161.46 g/20g BW followed by MSG 4 mg/g BW. All treatments were carried out orally for 52 days. This study was observed tubular epithelial hydropic degeneration, epithelial necrosis, and glomerular necrosis. Data were analyzed using Kruskal-Wallis test followed by the Mann-Whitney test. As a result, for the epithelial and glomerular necrosis variables, the T3 group showed significant results when compared with C+, T1, and T2 groups. Moreover, T3 was not significantly different from the C- group. It can be concluded that A. dorsata honey in T3 group could protect mice kidneys from the damaging effect of MSG.
Al-Anshori, A. A., Putri, A. N., Ismi, A. N., Suhud, M. K., Plumeriastuti, H., & Maslachah, L. (2023). Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats. Jurnal Medik Veteriner, 6(2), 145–154.
Al-Zuhroh, T. Y., Santoso, K. P., Yunita, M. N., Hidajati, N., & Praja, R. N. (2021). Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea Canephora). Jurnal Medik Veteriner, 4(2), 213–220.
Anbarkeh, F. R., Baradaran, R., Ghandy, N., Jalali, M., Nikravesh, M. R., & Soukhtanloo, M. (2019). Effects of Monosodium Glutamate on Apoptosis of Germ Cells in Testicular Tissue of Adult Rat: An Experimental Study. International Journal of Reproductive BioMedicine, 17(4), 261–70.
Arimbi, R., Darsono, H., Plumeriastuti, T. V., Widiyanto., & Legowo, D. (2015). Buku Ajar Patologi Umum Veteriner Edisi 2. Airlangga University Press Surabaya. pp: 34.
Erlangga, M. B., Srianto, P., Safitri, E., Hernawati, T., & Hermadi, H. A. (2023). The Potential of Kaliandra Honey (Calliandra sp.) on Sertoli Cell Counts in Malnourished Albino Rats. Jurnal Medik Veteriner, 6(1), 88–92.
Fikri, F., Purnomo, A., Chhetri, S., & Purnama, M. (2023). Sea Cucumber-Based Hydroxyapatite-Chitosan Ameliorate Serum Liver Enzymes and Cytokine Levels in Albino Rats with Femoral Bone Defect. Indian Veterinary Journal, 100(7), 23–26.
Flecknell, P. (2015). Laboratory Animal Anaesthesia (4th Ed). The Boulevard, Langford, Kidlington Oxford: Academic Press.
Fukuda, M., Kobayashi, K., Hirono, Y., Miyagawa, M., Ishida, T., Ejiogu, E. C., Sawai, M., Pinkerton, K. E., & Takeuchi, M. (2011). Jungle Honey Enhances Immune Function and Antitumor Activity. Evidence-Based Complementary and Alternative Medicine, 1–8.
Hamid, I. S., Fikri, F., Purnama, M. T. E., Solfaine, R., & Chhetri, S. (2022). Effects of Tithonia diversifolia on blood glucose levels, renal and pancreatic histopathology of Wistar rats: A model of diabetic nephropathy. Indian Veterinary Journal, 99(11), 37–39.
Himawan, R., Primarizky, H., Mafruchati, M., Triakoso, N., & Maslachah, L. (2021). Green Tea Leaves Extract Effect on Histopathology of Mercury Chloride Induced Rat's Liver. Pollution Research, 40(1), 326–329.
Jarrar, B. M. (2013). Histological and Histochemical Alterations in the Kidney Induced by Lead. Annals of Saudi Medicine, 23(2), 5–10.
Listyorini, L., Mustofa, I., Hernawati, T., Rimayanti, R., & Suprayogi, T. W. (2021). Honey Can Increase the Length of the Small Intestinal Villi in Malnourished Albino Rats. Jurnal Medik Veteriner, 4(2), 175–179.
Liwikasari, N. (2018). Pengaruh Vitamin C Terhadap Peroksidasi Lipid, Gejala Klinik dan Kualitas Hidup Penderita Tonsilitis Kronik. Journal of Clinical Medicine, 5(2).
Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free Radicals, Antioxidants, and Function Foods: Impact on Human Health. Pharmacognosy Reviews, 4(8), 118–128.
Miller, M. A., & Zachary, J. F. (2017). Pathologic Basis of Veterinary Disease – Expert Consult, 6th Edition. Elsevier inc. pp: 1–80.
Moniruzzaman, M., Khalil, M. I., Sulaiman, S. A., & Gan, S. H. (2013). Physicochemical and Antioxidant Properties of Malaysian Honeys Produced by Apis cerana, Apis dorsata, and Apis mellifera. BMC Complementary and Alternative Medicine, 13(1), 13–43
Muawanah, A., & Wardhani, P. (2015). Aktivitas Antikanker dan Antioksidan Madu di Pasaran Lokal Indonesia. Jurnal Ilmu Pertanian Indonesia, 19(3), 136–144.
Plessis, E. C. D., Prozesky, L., & Botha, C. J. (2011). The Pathology of Acure Nolletia Gariepina Poisoning of Cattle. Journal of the South African Veterinary Association, 82(3), 144–149.
Prasetyo, R. H., & Safitri, E. (2016). Effects of honey to mobilize endogenous stem cells in efforts intestinal and ovarian tissue regeneration in rats with protein energy malnutrition. Asian Pacific Journal of Reproduction, 5(3), 198–203.
Safitri, E., Purnobasuki, H., Purnama, M. T. E., & Chhetri, S. (2022). Effectiveness of forest honey (Apis dorsata) as therapy for ovarian failure causing malnutrition. F1000Research, 11, 512.
Saputri, Dinar, S., & Yolli, E. P. (2017). Aktivitas Antioksidan Madu Hutan di Beberapa Kecamatan di Kabupaten Sumbawa Besar. Jurnal Tambora, 2(3).
Sayuti, K., & Rina, Y. (2015). Antioksidan Alami dan Sintetik, Andalas University Press, Padang.
Sharma, A. (2015). Monosodium Glutamate-Induced Oxidative Kidney Damage and Possible Mechanisms: A Mini-Review. Journal of Biomedical Science, 22(1).
Sharma, A., Wongkham, C., Prasongwattana, V., Boonnate, P., Thanan, R., Reungjui, S., & Cha'on, U. (2014). Proteomic Analysis of Kidney in Rats Chronically Exposed to Monosodium Glutamate. PlosOne, 9(12), e116233.
Suarjaya, I. P. P., Tatang, B., & Himendra, W. (2012). Reactive Oxygen Species pada Cedera Otak Traumatik. Jurnal Neuroanestesi Indonesia, 1(2), 144–150.
Weliyani, T., Hamid, I. S., Hidanah, S., Prastiya, R. A., & Wibawati, P. A. (2021). Effect of Robusta Coffee Extract on Histopathological in Mice Testes Induced with Monosodium Glutamat. Jurnal Medik Veteriner, 4(2), 243–248.
Werdhasari, A. (2014). Peran Antioksidan bagi Kesehatan. Jurnal Biotek Medisiana Indonesia, 3(2), 59–68.
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